{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T08:45:58Z","timestamp":1769589958310,"version":"3.49.0"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union and Junta de Castilla-La Mancha","award":["SBPLY\/21\/180501\/000257"],"award-info":[{"award-number":["SBPLY\/21\/180501\/000257"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Age-related changes increase frailty and vulnerability to stress in older adults, and stress has been linked to poorer balance and fall risk. However, the mechanisms by which emotional stress may contribute to falls remain unclear. This study explores whether: 1) emotional stressors lead to neuromuscular changes that affect postural control in older adults; and 2) technology can assist in fall prevention by detecting increased risk. A cross-sectional, laboratory-based observational study is being conducted in a single session comparing 30 young adults (18\u201339 years) and 30 older adults (\u226565 years), in which participants are exposed to emotional stressors (high-arousal images) and their immediate neuromuscular and balance responses are recorded. The first participant was enrolled on November 27, 2024. All participants complete a sequence of physical tasks under two conditions: viewing low-arousal and high-arousal images from the International Affective Picture System. The physical tasks involve standing up from a chair, walking to a table, transferring water between bottles, returning to the chair, and sitting down. Emotional responses are assessed using heart rate variability, respiratory rate and subjective feelings of unease. Electromyographic signals are analysed using wearable sensors, ground pressure is recorded via pressure sensors and bottle manipulation is tracked with inertial measurement units. Furthermore, balance is evaluated using the Timed Up and Go Test and the Functional Reach Test, administered before the low-arousal condition and after the high-arousal condition. Comprehensive data analysis will provide new insights to aid professionals in designing interventions for detecting and preventing fall risk in older adults. The study was approved by the corresponding ethics committee and registered at ClinicalTrials.gov (NCT06682754) on November 21, 2024. It is being conducted at the University of Alcal\u00e1, Spain, and is funded by the European Union and the \u2018Junta de Castilla-La Mancha\u2019. Principal investigators are Dr. Bernardo Alarcos and Dr. Susana Nunez-Nagy (\n                    <jats:email xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:type=\"simple\">susana.nunez@uah.es<\/jats:email>\n                    ). The collected data will be anonymized and shared in an open-access repository, in line with ethical and open science principles.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0341744","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T18:43:58Z","timestamp":1769539438000},"page":"e0341744","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of balance in the elderly under the influence of stress (DEPIE): A cross-sectional study protocol"],"prefix":"10.1371","volume":"21","author":[{"name":"Membership of GITES","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernardo","family":"Alarcos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bel\u00e9n","family":"D\u00edaz-Pulido","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olalla","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Fern\u00e1ndez-Guinea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Garc\u00eda Herraiz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6234-5907","authenticated-orcid":true,"given":"Mar\u00eda del Mar","family":"Lend\u00ednez-Chica","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Mart\u00ednez Muela","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9206-6725","authenticated-orcid":true,"given":"Susana","family":"Nunez-Nagy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yolanda","family":"P\u00e9rez-Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Rodr\u00edguez-Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gloria M.","family":"Rubio Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0551-4188","authenticated-orcid":true,"given":"Sara","family":"Trapero-Asenjo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan R.","family":"Velasco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,1,27]]},"reference":[{"key":"pone.0341744.ref001","volume-title":"Decade of Healthy Ageing 2020-2030","author":"World Health Organization","year":"2020"},{"key":"pone.0341744.ref002","unstructured":"Eurostat: Population structure indicators [Internet]. Luxembbourg: European Commission, Eurostat; 2025. [cited 2025 May 21]. Available from: https:\/\/ec.europa.eu\/eurostat\/databrowser\/view\/DEMO_PJANIND__custom_16801577\/default\/table?lang=en"},{"key":"pone.0341744.ref003","volume-title":"Informe mundial sobre el envejecimiento y la salud","author":"World Health Organization","year":"2015"},{"issue":"2","key":"pone.0341744.ref004","article-title":"Biomarkers of ageing: current state\u2010of\u2010art, challenges, and opportunities","volume":"2","author":"R Chen","year":"2023","journal-title":"MedComm \u2013 Fut Med"},{"issue":"3","key":"pone.0341744.ref005","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.jamda.2015.12.016","article-title":"Frailty: an emerging public health priority","volume":"17","author":"M Cesari","year":"2016","journal-title":"J Am Med Dir Assoc"},{"key":"pone.0341744.ref006","volume-title":"WHO Global report on falls prevention in older age","author":"World Health Organization","year":"2007"},{"key":"pone.0341744.ref007","unstructured":"World Health Organization: Falls [Internet]. 2021 [cited 2025 May 21]. https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/falls\/"},{"issue":"6","key":"pone.0341744.ref008","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.regg.2015.05.005","article-title":"Ca\u00eddas en la poblaci\u00f3n anciana espa\u00f1ola: Incidencia, consecuencias y factores de riesgo","volume":"50","author":"A Rodr\u00edguez-Molinero","year":"2015","journal-title":"Rev Esp Geriatr Gerontol"},{"issue":"2","key":"pone.0341744.ref009","doi-asserted-by":"crossref","first-page":"714","DOI":"10.14336\/AD.2024.0124-1","article-title":"Age-related dysfunction in balance: a comprehensive review of causes, consequences, and interventions","volume":"16","author":"J Wang","year":"2024","journal-title":"Aging Dis"},{"issue":"1","key":"pone.0341744.ref010","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1093\/ageing\/aft117","article-title":"Association of stressful life events with incident falls and fractures in older men: the Osteoporotic Fractures in Men (MrOS) Study","volume":"43","author":"HA Fink","year":"2014","journal-title":"Age Ageing"},{"key":"pone.0341744.ref011","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1471-2318-9-7","article-title":"Emotional stress as a trigger of falls leading to hip or pelvic fracture. Results from the ToFa study - a case-crossover study among elderly people in Stockholm, Sweden","volume":"9","author":"J M\u00f6ller","year":"2009","journal-title":"BMC Geriatr"},{"key":"pone.0341744.ref012","doi-asserted-by":"crossref","first-page":"111495","DOI":"10.1016\/j.exger.2021.111495","article-title":"Examining the influence of mental stress on balance perturbation responses in older adults","volume":"153","author":"RY Akinlosotu","year":"2021","journal-title":"Exp Gerontol"},{"key":"pone.0341744.ref013","doi-asserted-by":"crossref","first-page":"487","DOI":"10.47197\/retos.v61.105987","article-title":"Relaci\u00f3n entre el estr\u00e9s percibido, desempe\u00f1o f\u00edsico y equilibrio de personas mayores. 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Actualizaci\u00f3n del documento de consenso sobre prevenci\u00f3n de la fragilidad en la persona mayor [Update of the consensus document on frailty prevention in older people] [Internet]. Madrid: Ministerio de Sanidad; 2022 [cited 2025 Dec 16]. Available from: https:\/\/www.sanidad.gob.es\/"},{"issue":"1","key":"pone.0341744.ref028","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1186\/s12877-020-01724-3","article-title":"Validity and reliability testing of the Spanish version of the BESTest and mini-BESTest in healthy community-dwelling elderly","volume":"20","author":"P Dominguez-Olivan","year":"2020","journal-title":"BMC Geriatr"},{"issue":"2","key":"pone.0341744.ref029","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1111\/j.1532-5415.1991.tb01616.x","article-title":"The timed \u201cUp & Go\u201d: a test of basic functional mobility for frail elderly persons","volume":"39","author":"D Podsiadlo","year":"1991","journal-title":"J Am Geriatr Soc"},{"issue":"2","key":"pone.0341744.ref030","first-page":"82","article-title":"Validez y reproducibilidad de medidas de evaluaci\u00f3n funcional basadas en la ejecuci\u00f3n en personas mayores [Validity and reproducibility of functional assessment based on performance status]","volume":"35","author":"CL Curcio","year":"2000","journal-title":"Rev Esp Geriatr Gerontol"},{"issue":"11","key":"pone.0341744.ref031","doi-asserted-by":"crossref","first-page":"4010","DOI":"10.3390\/s18114010","article-title":"Measuring sedentary behavior by means of muscular activity and accelerometry","volume":"18","author":"RP Kuster","year":"2018","journal-title":"Sensors (Basel)"},{"issue":"2","key":"pone.0341744.ref032","first-page":"13","article-title":"European recommendations for surface electromyography results of the SENIAM project","volume":"8","author":"HJ Hermens","year":"1999","journal-title":"Enschede: Roessingh Res Dev"},{"issue":"1","key":"pone.0341744.ref033","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s12877-018-0706-6","article-title":"Novel sensing technology in fall risk assessment in older adults: a systematic review","volume":"18","author":"R Sun","year":"2018","journal-title":"BMC Geriatr"},{"key":"pone.0341744.ref034","volume-title":"International Affective Picture System (IAPS): Technical Manual and Affective Ratings","author":"PJ Lang","year":"1997"},{"issue":"3","key":"pone.0341744.ref035","article-title":"Adaptaci\u00f3n eapa\u00f1ola del \u201cInternational Affective Picture System\u201d (IAPS). Tercera parte","volume":"29","author":"J Molt\u00f3","year":"2013","journal-title":"Anal Psicol"},{"issue":"2","key":"pone.0341744.ref036","doi-asserted-by":"crossref","first-page":"512","DOI":"10.3758\/BRM.40.2.512","article-title":"Age-related differences in valence and arousal ratings of pictures from the International Affective Picture System (IAPS): do ratings become more extreme with age?","volume":"40","author":"D Gr\u00fchn","year":"2008","journal-title":"Behav Res Methods"},{"issue":"7","key":"pone.0341744.ref037","doi-asserted-by":"crossref","first-page":"2750","DOI":"10.1038\/s41380-023-01986-4","article-title":"Neurocognitive effects of stress: a metaparadigm perspective","volume":"28","author":"EJ Kim","year":"2023","journal-title":"Mol Psychiatry"},{"issue":"4","key":"pone.0341744.ref038","doi-asserted-by":"crossref","first-page":"385","DOI":"10.2307\/2136404","article-title":"A global measure of perceived stress","volume":"24","author":"S Cohen","year":"1983","journal-title":"J Health Soc Behav"},{"issue":"8","key":"pone.0341744.ref039","article-title":"Development of artificial intelligence edge computing based wearable device for fall detection and prevention of elderly people","volume":"10","author":"A Paramasivam","year":"2024","journal-title":"Heliyon"},{"key":"pone.0341744.ref040","first-page":"100614","article-title":"AI based elderly fall prediction system using wearable sensors: a smart home-care technology with IOT","volume":"25","author":"P Kulurkar","year":"2023","journal-title":"Measurement: Sensors"},{"key":"pone.0341744.ref041","doi-asserted-by":"crossref","first-page":"114186","DOI":"10.1016\/j.measurement.2024.114186","article-title":"A comprehensive review of elderly fall detection using wireless communication and artificial intelligence techniques","volume":"226","author":"SK Gharghan","year":"2024","journal-title":"Measurement"},{"issue":"4","key":"pone.0341744.ref042","article-title":"Predicting fall risk through automatic wearable monitoring: a systematic review","volume":"12","author":"MC Olson","year":"2021","journal-title":"Int J Progn Health Manag"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0341744","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T18:44:05Z","timestamp":1769539445000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0341744"}},"subtitle":[],"editor":[{"given":"Shu","family":"Morioka","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,1,27]]},"references-count":42,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0341744","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,27]]}}}