{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T12:51:26Z","timestamp":1770468686506,"version":"3.49.0"},"reference-count":28,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011568","name":"Huck Institutes of the Life Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011568","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["PEN04853"],"award-info":[{"award-number":["PEN04853"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["Multistate Project 4666"],"award-info":[{"award-number":["Multistate Project 4666"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Lester Earl and Veronica Casida Career Development Endowment"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Sepsis is a life-threatening state of disseminated infection, and treatment requires knowledge of the organism responsible. The gold standard for sepsis diagnosis is blood culture, which requires days of growth. Next-generation sequencing has been proposed as an alternative; however, existing methods may lack sensitivity. In this work, we explore the idea of genomic quasi-G-primes, which are short DNA sequences specific to a single species within a group of relevant species. We first validated the genomic quasi-G-prime classification in controlled\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    sequencing experiments, and then applied the same approach to blood-derived sequencing data from septic and healthy patients, where genomic quasi-G-prime profiles distinguished disease states. Our method is highly space-efficient, permitting fast classification on modest hardware and enabling it to outperform existing taxonomic classification approaches in this task.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0341828","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T18:34:06Z","timestamp":1770402846000},"page":"e0341828","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Doing more with less: Genomic quasi-G-primes differentiate septic from healthy patients"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5301-9459","authenticated-orcid":true,"given":"Congzhou 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