{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:38:08Z","timestamp":1792802288603,"version":"4.5.0"},"reference-count":145,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University Research Council (URC) Multidisciplinary Research Grant, University of Peradeniya","award":["516"],"award-info":[{"award-number":["516"]}],"id":[{"id":"https:\/\/ror.org\/025h79t26","id-type":"ROR","asserted-by":"crossref"}]},{"name":"University Research Council (URC) Multidisciplinary Research Grant, University of Peradeniya","award":["516"],"award-info":[{"award-number":["516"]}],"id":[{"id":"https:\/\/ror.org\/025h79t26","id-type":"ROR","asserted-by":"crossref"}]},{"name":"University Research Council (URC) Multidisciplinary Research Grant, University of Peradeniya","award":["516"],"award-info":[{"award-number":["516"]}],"id":[{"id":"https:\/\/ror.org\/025h79t26","id-type":"ROR","asserted-by":"crossref"}]},{"name":"University Research Council (URC) Multidisciplinary Research Grant, University of Peradeniya","award":["516"],"award-info":[{"award-number":["516"]}],"id":[{"id":"https:\/\/ror.org\/025h79t26","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    African swine fever virus (ASFV) is a highly lethal DNA virus that suppresses the host\u2019s immune response by establishing infection. B175L, one of its key immune-evasion proteins, directly inhibits STING-mediated type I interferon (IFN-I) signalling, thereby preventing the activation of antiviral defences. Thus, targeting B175L could be a promising strategy for antiviral drug development as effective ASFV inhibitors remain unidentified. In this study, we investigated the potential of\n                    <jats:italic>Tinospora cordifolia<\/jats:italic>\n                    \u2019s bioactive compounds to disrupt B175L\u2019s function and restore immune signalling. Gas chromatography-mass spectrometry (GC-MS) analysis of the methanol extract from\n                    <jats:italic>T. cordifolia<\/jats:italic>\n                    stems identified 86 compounds. These were filtered using SwissADME, ProTox 3.0, and DataWarrior, yielding 15 compounds with favourable drug-likeness and safety profiles. We generated a highly accurate 3D model of ASFV B175L with strong confidence scores for the structural accuracy, using AlphaFold3. The filtered compounds were then subjected to virtual screening with PyRx 0.8, and the 3 compounds with binding affinities\u2009\u2264\u2009\u22126 kcal\/mol were selected for subsequent analysis. Molecular dynamics (MD) simulations were used to assess binding stability, including root mean square deviation and fluctuation (RMSD, RMSF), protein-ligand contacts, radius of gyration (rGyr), and solvent accessible surface area (SASA) using Schr\u00f6dinger Maestro. Taken together, these\n                    <jats:italic>in silico<\/jats:italic>\n                    results suggest that\n                    <jats:italic>T. cordifolia-<\/jats:italic>\n                    derived Benzaldehyde, 5-bromo-2-hydroxy-, (5-trifluoromethyl-2-pyridyl) hydrazone, Carbamic acid, N-(3-oxo-4-isoxazolidinyl)-, benzyl ester, and 1H-Indol-5-ol may act as potential inhibitors of B175L and represent preliminary antiviral hits against ASFV, warranting further\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    validation.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0341938","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T17:41:43Z","timestamp":1780508503000},"page":"e0341938","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["In silico pharmacological analysis of Tinospora cordifolia compounds targeting African swine fever virus B175L"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3139-3326","authenticated-orcid":true,"given":"Kasuni","family":"Karunarathne","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saumya","family":"Poorni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5870-2440","authenticated-orcid":true,"given":"Nethra","family":"Jayampathi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2756-6296","authenticated-orcid":true,"given":"Hasitha","family":"Dhananjaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8203-8124","authenticated-orcid":true,"given":"Anju","family":"Hasintha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2155-5972","authenticated-orcid":true,"given":"Malinda","family":"Hulugalla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thilini A.N.","family":"Mahakapuge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadeeka","family":"Nethmini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2619-8719","authenticated-orcid":true,"given":"Naveed","family":"Iqbal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barana","family":"Jayawardana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2904-4018","authenticated-orcid":true,"given":"Lakmal","family":"Ranathunga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,6,3]]},"reference":[{"issue":"1","key":"pone.0341938.ref001","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1080\/22221751.2022.2108342","article-title":"African swine fever control and prevention: an update on vaccine development","volume":"11","author":"AC Urbano","year":"2022","journal-title":"Emerg Microbes Infect"},{"issue":"11","key":"pone.0341938.ref002","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.3390\/pathogens10111357","article-title":"Virucidal Activity of Plant Extracts against African Swine Fever Virus","volume":"10","author":"M Juszkiewicz","year":"2021","journal-title":"Pathogens"},{"key":"pone.0341938.ref003","unstructured":"World Organisation for Animal Health, F A O. 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