{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T23:24:28Z","timestamp":1770765868769,"version":"3.50.0"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Regional Scholarship and Innovation Fund (RSIF) of the Partnership for Skills in Applied Sciences, Engineering and Technology","award":["Project Grant No. P165581"],"award-info":[{"award-number":["Project Grant No. P165581"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"\n Background<\/jats:title>\n Bovine tuberculosis (BTB) has a significant impact on both the cattle industry and human health. Understanding its transmission, clinical signs, risk factors, and control measures is essential for prevention. This study aimed to evaluate the knowledge, attitudes, and practices regarding BTB in high-burden areas of Malawi.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n A stratified sampling design was employed to collect data from 463 butchers, dairy farmers and cattle farmers in different locations and settings in Malawi. Aggregate scores on knowledge, attitudes, and practices were taken as multivariate measurements and fitted with multivariate linear regression models.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Participants displayed satisfactory knowledge (88.68%), negative attitudes towards risky behaviours (92.27%), and appropriate practices (86.83%) concerning BTB. Most were aware of its presence in cattle (85.71%) and potential transmission to humans (74.09%), yet exhibited gaps in understanding clinical signs and held misconceptions about hereditary transmission. Many participants demonstrated risky behaviours, such as consuming raw animal products and selling products from infected animals. Individuals with secondary (\u03b2: \u22122.148; 95% CI: \u22124.168, \u22120.127) and tertiary education (\u03b2: \u22123.488; 95% CI: \u22126.626, \u22120.349) exhibited more negative attitudes towards risky behaviours compared to those with informal education. Adults aged 18\u221230 (\u03b2: \u22122.777; 95% CI: \u22125.469, \u22120.085) and those aged 31\u221245 (\u03b2: \u22123.035; 95% CI: \u22125.752, \u22120.319) demonstrated better protective practices than youths under 18.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n There is an urgent need for targeted health education on BTB, focusing on clinical signs and the myth of hereditary transmission, particularly aimed at young people, individuals with limited formal education, and farmers, to enhance prevention efforts.<\/jats:p>\n <\/jats:sec>","DOI":"10.1371\/journal.pone.0341968","type":"journal-article","created":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:30:35Z","timestamp":1770748235000},"page":"e0341968","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Knowledge, attitudes and practices regarding bovine tuberculosis in cattle and humans in Malawi"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7026-4894","authenticated-orcid":true,"given":"Alfred","family":"Ngwira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel O.M.","family":"Manda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esron D.","family":"Karimuribo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharadhuli I.","family":"Kimera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,2,10]]},"reference":[{"issue":"2","key":"pone.0341968.ref001","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2023.e13647","article-title":"Prevalence and risk factors of bovine tuberculosis in slaughtered cattle, Malawi","volume":"9","author":"TF Kapalamula","year":"2023","journal-title":"Heliyon"},{"issue":"1","key":"pone.0341968.ref002","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s42522-024-00130-8","article-title":"Meta-analysis of the prevalence of tuberculosis in cattle and zoonotic tuberculosis in humans in sub-Saharan Africa","volume":"7","author":"A Ngwira","year":"2025","journal-title":"One Health Outlook"},{"key":"pone.0341968.ref003","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-030-18690-6_16","article-title":"The status of bovine tuberculosis in Malawi","volume-title":"Tuberculosis in animals: an African perspective","author":"PE Njoka","year":"2019"},{"issue":"6","key":"pone.0341968.ref004","doi-asserted-by":"crossref","first-page":"899","DOI":"10.3201\/eid1906.120543","article-title":"Zoonotic Mycobacterium bovis-induced tuberculosis in humans","volume":"19","author":"B M\u00fcller","year":"2013","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"pone.0341968.ref005","doi-asserted-by":"crossref","first-page":"41","DOI":"10.2478\/fv-2019-0006","article-title":"Prevalence and economic losses due to bovine tuberculosis in cattle slaughtered at Bodija municipal abattoir, Ibadan, Nigeria","volume":"63","author":"OD Adelakun","year":"2019","journal-title":"Folia Veterinaria"},{"key":"pone.0341968.ref006","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/5584_2018_170","article-title":"Incidence and drug resistance of zoonotic Mycobacterium bovis infection in Peshawar, Pakistan","volume":"1057","author":"I Khattak","year":"2018","journal-title":"Adv Exp Med Biol"},{"issue":"1","key":"pone.0341968.ref007","doi-asserted-by":"crossref","first-page":"163","DOI":"10.2478\/pneum-2024-0023","article-title":"Extrapulmonary tuberculosis: challenges in diagnosis and treatment - 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2015","volume":"13","author":"HK Adesokan","year":"2018","journal-title":"PLoS One"},{"key":"pone.0341968.ref024","first-page":"1025","article-title":"Knowledge and practice for prevention of bovine tuberculosis and Its derivers among HIV positive people in Bahir Dar city public hospitals, Ethiopia","volume":"13","author":"FA Hailu","year":"2021","journal-title":"HIV AIDS (Auckl)"},{"issue":"1","key":"pone.0341968.ref025","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0146538","article-title":"Knowledge of bovine tuberculosis, cattle husbandry and dairy practices amongst pastoralists and small-scale dairy farmers in Cameroon","volume":"11","author":"RF Kelly","year":"2016","journal-title":"PLoS One"},{"key":"pone.0341968.ref026","first-page":"163","article-title":"Public Awareness, Prevalence and potential determinants of bovine tuberculosis in selected districts of Gamo zone, southern Ethiopia","volume":"13","author":"E Tora","year":"2022","journal-title":"Vet Med (Auckl)"},{"issue":"5","key":"pone.0341968.ref027","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1093\/occmed\/kqw039","article-title":"Zoonotic tuberculosis in occupationally exposed groups in Pakistan","volume":"66","author":"I Khattak","year":"2016","journal-title":"Occup Med (Lond)"},{"key":"pone.0341968.ref028","doi-asserted-by":"crossref","first-page":"121","DOI":"10.2147\/JMDH.S97409","article-title":"Knowledge and perception about tuberculosis among children attending primary school in Ntcheu District, Malawi","volume":"9","author":"P Nyasulu","year":"2016","journal-title":"J Multidiscip Healthc"},{"key":"pone.0341968.ref029","doi-asserted-by":"crossref","first-page":"375","DOI":"10.2147\/JMDH.S156949","article-title":"Knowledge, beliefs, and perceptions of tuberculosis among community members in Ntcheu district, Malawi","volume":"11","author":"P Nyasulu","year":"2018","journal-title":"J Multidiscip Healthc"},{"issue":"6","key":"pone.0341968.ref030","doi-asserted-by":"crossref","first-page":"649","DOI":"10.3390\/pathogens11060649","article-title":"Mycobacterium bovis tuberculosis in two goat farms in multi-host ecosystems in Sicily (Italy): epidemiological, diagnostic, and regulatory considerations","volume":"11","author":"V Di Marco Lo Presti","year":"2022","journal-title":"Pathogens"},{"key":"pone.0341968.ref031","doi-asserted-by":"crossref","first-page":"169","DOI":"10.3389\/fvets.2018.00169","article-title":"Risk perceptions and protective behaviors toward bovine tuberculosis among abattoir and butcher workers in Ethiopia","volume":"5","author":"F Fekadu","year":"2018","journal-title":"Front Vet Sci"},{"issue":"1","key":"pone.0341968.ref032","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s40249-017-0279-1","article-title":"Post-2015, why delay to seek healthcare? 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