{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T19:17:21Z","timestamp":1771355841181,"version":"3.50.1"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007637","name":"Departamento Administrativo de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["221384467683"],"award-info":[{"award-number":["221384467683"]}],"id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    The\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    genus comprises fungi that are widely distributed in nature. Several\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    sections are important for recycling organic matter; while the\n                    <jats:italic>Fumigati<\/jats:italic>\n                    section is classically associated with compost, the\n                    <jats:italic>Terrei<\/jats:italic>\n                    section also plays a relevant role in this process.\n                    <jats:italic>Aspergillus hortae<\/jats:italic>\n                    is a species from this section that has been isolated from clinical samples, but its role as a pathogenic agent is unclear. In this study, we analysed three clinical isolates of\n                    <jats:italic>A. hortae<\/jats:italic>\n                    from Colombia, initially misidentified as\n                    <jats:italic>A. terreus<\/jats:italic>\n                    . Morphological characterization at 26 \u00b0C and 37 \u00b0C confirmed its thermotolerant nature. Whole-genome sequencing enabled accurate species identification and revealed phylogenetic divergence from the reference genome. Antifungal susceptibility testing (EUCAST broth microdilution) showed intrinsic resistance to amphotericin B (MIC 2\u20134 mg\/L) and susceptibility to azoles (MIC 0.25\u20131 mg\/L). A mutation (M769K) in the MelA ortholog of a hypopigmented isolate, which had the lowest MIC value for AmB (2 mg\/L), was identified. This study presents the morphological characterization, molecular typing through whole-genome analysis and identification of susceptibility profiles to azoles and amphotericin B of three clinical isolates of\n                    <jats:italic>A. hortae<\/jats:italic>\n                    from Colombia.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0342479","type":"journal-article","created":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T18:33:30Z","timestamp":1771353210000},"page":"e0342479","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Whole-genome sequencing, phenotypic characterization, and antifungal susceptibility profiles of three Aspergillus hortae clinical isolates from Colombia"],"prefix":"10.1371","volume":"21","author":[{"given":"Santiago","family":"Marin-Carvajal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana","family":"Quiceno Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Camila","family":"Zuleta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1lvaro","family":"R\u00faa-Giraldo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9189-764X","authenticated-orcid":true,"given":"Ana 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