{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T19:22:06Z","timestamp":1771615326034,"version":"3.50.1"},"reference-count":74,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:00:00Z","timestamp":1771545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"crossref","award":["23\/17188-4"],"award-info":[{"award-number":["23\/17188-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"crossref","award":["23\/17182-6"],"award-info":[{"award-number":["23\/17182-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Federal University of Mato Grosso do Sul\u2014UFMS\/MEC\u2014Brazil"},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"crossref","award":["22\/15264-2"],"award-info":[{"award-number":["22\/15264-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Scorpions are arthropods with venom glands at their telson that produce chemicals such as peptides and proteins. These compounds may have pharmacological effects, including antimicrobial, ion channel modulating, and antihypertensive activities. Our study aims to examine the transcripts from the venom glands of\n                    <jats:italic>Tityus paraguayensis<\/jats:italic>\n                    , focusing on identifying and annotating the genes expressed in these glands. A transcript encoding a potassium channel-modulating peptide was selected for 3D structural modeling, phylogenetic analysis, and interaction assessment. Initially, the scorpions\u2019 telsons were dissected and analyzed using transcriptome sequencing. The data were then assembled and functionally annotated. The sequencing and assembly of the venom gland transcriptome produced a set of 37,283 transcripts, of which 523 were annotated as potentially related to venom components. Among the venom components, peptides that modulate sodium (8%), potassium (9%), and calcium (1%) channels, antimicrobial peptides (6%), antihypertensives (2%), phospholipases (1%), and metalloproteinases (29%) were identified, along with other compounds (44%). Specific highlights include the structural-functional analysis of four key peptides: TpNa3, a probable \u03b2-toxin sodium channel modulator with a \u03b2\u03b1\u03b2\u03b2 structural motif; TpHyp1, a long-chain antihypertensive peptide that contains the conserved KPP motif; TpAP1, a short antimicrobial peptide with a low positive charge and an \u03b1-helical structure; and TpK8 is a potassium toxin that was previously partly identified (\u03b1-KTx). Molecular modeling and docking analyses showed that TpK8 binds with high affinity and stability, especially to the Kv1.3 channel, through specific interactions with the selectivity filter. These findings emphasize the extensive molecular diversity of\n                    <jats:italic>T. paraguayensis<\/jats:italic>\n                    venom, highlighting its potential as a rich and largely unexplored source of bioactive molecules. This makes it a promising target for developing new bioactive compounds for biotechnological and therapeutic use.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0343107","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T18:35:18Z","timestamp":1771612518000},"page":"e0343107","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["The venom gland transcriptome of Tityus paraguayensis reveals a diverse array of bioactive molecules from the Brazilian Cerrado"],"prefix":"10.1371","volume":"21","author":[{"given":"Henrique Ranieri","family":"Covali-Pontes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brayhan","family":"Meneguelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00e9ssica de Moraes","family":"Carretone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alynne Coelho","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8156-4059","authenticated-orcid":true,"given":"Ang\u00e9lica Camargo dos","family":"Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thais Fernanda","family":"Carlos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Roberto","family":"Chiaratti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milene","family":"Ferro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3329-4597","authenticated-orcid":true,"given":"Fl\u00e1vio Henrique","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata dos Santos","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2566-5642","authenticated-orcid":true,"given":"Malson Neilson","family":"Lucena","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,2,20]]},"reference":[{"key":"pone.0343107.ref001","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.toxicon.2015.06.029","article-title":"Overview of the Knottin scorpion toxin-like peptides in scorpion venoms: insights on their classification and evolution","volume":"107","author":"CE Santib\u00e1\u00f1ez-L\u00f3pez","year":"2015","journal-title":"Toxicon"},{"key":"pone.0343107.ref002","volume-title":"The scorpion files","author":"JO Rein","year":"2025"},{"key":"pone.0343107.ref003","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s13127-019-00390-7","article-title":"Exploring the evolution and terrestrialization of scorpions (Arachnida: Scorpiones) with rocks and clocks","volume":"19","author":"RJ Howard","year":"2019","journal-title":"Org Divers Evol"},{"issue":"1","key":"pone.0343107.ref004","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1678-9199-20-8","article-title":"A historical approach to scorpion studies with special reference to the 20th and 21st centuries","volume":"20","author":"WR Louren\u00e7o","year":"2014","journal-title":"J Venom Anim Toxins Incl Trop Dis"},{"key":"pone.0343107.ref005","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1071\/IS14035","article-title":"Shining a light into the world\u2019s deepest caves: phylogenetic systematics of the troglobiotic scorpion genus Alacran Francke, 1982 (Typhlochactidae: Alacraninae)","volume":"28","author":"CE Santib\u00e1\u00f1ez-L\u00f3pez","year":"2014","journal-title":"Invertebr Syst"},{"issue":"3","key":"pone.0343107.ref006","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/s00134-020-05924-8","article-title":"Scorpion envenomation: state of the art","volume":"46","author":"F Abroug","year":"2020","journal-title":"Intensive Care Med"},{"issue":"2","key":"pone.0343107.ref007","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0011069","article-title":"Scorpion envenomation in Brazil: current scenario and perspectives for containing an increasing health problem","volume":"17","author":"C Guerra-Duarte","year":"2023","journal-title":"PLoS Negl Trop Dis"},{"key":"pone.0343107.ref008","author":"Sinan","year":"2025"},{"key":"pone.0343107.ref009","first-page":"146","article-title":"Scorpion\u2019s biodiversity and proteinaceous components of venom","volume":"18","author":"N Akbar","year":"2021","journal-title":"Int J 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Proteomics"},{"issue":"1","key":"pone.0343107.ref013","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1080\/14789450.2020.1705158","article-title":"Scorpion venomics: a 2019 overview","volume":"17","author":"JI Cid-Uribe","year":"2020","journal-title":"Expert Rev Proteomics"},{"key":"pone.0343107.ref014","author":"RB de Almeida","year":"2010","journal-title":"Atlas das esp\u00e9cies de Tityus C. L. Koch, 1836 (Scorpiones, Buthidae) do Brasil"},{"issue":"2","key":"pone.0343107.ref015","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0192070","article-title":"Insights into the origin of the high variability of multivalent-meiotic associations in holocentric chromosomes of Tityus (Archaeotityus) scorpions","volume":"13","author":"VF Mattos","year":"2018","journal-title":"PLoS One"},{"key":"pone.0343107.ref016","doi-asserted-by":"crossref","first-page":"108332","DOI":"10.1016\/j.toxicon.2025.108332","article-title":"Tityus paraguayensis, a scorpion from the Brazilian Cerrado: first assessment of venom and hemolymph composition and biological activity","volume":"258","author":"HR Covali-Pontes","year":"2025","journal-title":"Toxicon"},{"key":"pone.0343107.ref017","doi-asserted-by":"crossref","first-page":"108603","DOI":"10.1016\/j.toxicon.2025.108603","article-title":"Scorpion venom as a natural peptide source for innovative therapeutic solutions: A comprehensive review of its potential in emerging medical frontiers","volume":"268","author":"R Abdallnasser Amen","year":"2025","journal-title":"Toxicon"},{"key":"pone.0343107.ref018","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2017\/5245021","article-title":"Emerging and neglected infectious diseases: insights, advances, and challenges","volume":"2017","author":"N Nii-Trebi","year":"2017","journal-title":"Biomed Res Int"},{"issue":"2","key":"pone.0343107.ref019","doi-asserted-by":"crossref","first-page":"44","DOI":"10.3390\/antibiotics7020044","article-title":"Concepts and methods to access novel antibiotics from actinomycetes","volume":"7","author":"JJ Hug","year":"2018","journal-title":"Antibiotics (Basel)"},{"key":"pone.0343107.ref020","first-page":"7","article-title":"Cancer statistics, 2021","volume":"71","author":"RL Siegel","year":"2021","journal-title":"CA Cancer J Clin"},{"key":"pone.0343107.ref021","first-page":"1","volume-title":"What are omics sciences? Periparturient diseases of dairy cows: a systems biology approach","author":"M Vailati-Riboni","year":"2017"},{"issue":"1","key":"pone.0343107.ref022","doi-asserted-by":"crossref","DOI":"10.1530\/JME-18-0055","article-title":"Integrated omics: tools, advances and future approaches","volume":"62","author":"BB Misra","year":"2019","journal-title":"J Mol Endocrinol"},{"key":"pone.0343107.ref023","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s13059-016-0881-8","article-title":"A survey of best practices for RNA-seq data analysis","volume":"17","author":"A Conesa","year":"2016","journal-title":"Genome Biol"},{"key":"pone.0343107.ref024","doi-asserted-by":"crossref","first-page":"10","DOI":"10.3389\/fgene.2020.00220","article-title":"Editorial: RNA-seq analysis: methods, applications and challenges","volume":"11","author":"F Geraci","year":"2020","journal-title":"Front Genet"},{"issue":"7","key":"pone.0343107.ref025","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length transcriptome assembly from RNA-Seq data without a reference genome","volume":"29","author":"MG Grabherr","year":"2011","journal-title":"Nat Biotechnol"},{"issue":"10","key":"pone.0343107.ref026","doi-asserted-by":"crossref","first-page":"3420","DOI":"10.1093\/nar\/gkn176","article-title":"High-throughput functional annotation and data mining with the Blast2GO suite","volume":"36","author":"S G\u00f6tz","year":"2008","journal-title":"Nucleic Acids Res"},{"key":"pone.0343107.ref027","article-title":"Basic local alignment search tool","author":"SF Altschup","year":"1990","journal-title":"J Mol Biol"},{"key":"pone.0343107.ref028","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1186\/1471-2105-12-323","article-title":"RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome","volume":"12","author":"B Li","year":"2011","journal-title":"BMC Bioinform"},{"issue":"9","key":"pone.0343107.ref029","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1093\/bioinformatics\/17.9.847","article-title":"InterProScan--an integration platform for the signature-recognition methods in InterPro","volume":"17","author":"EM Zdobnov","year":"2001","journal-title":"Bioinformatics"},{"issue":"8","key":"pone.0343107.ref030","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1038\/nprot.2013.084","article-title":"De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis","volume":"8","author":"BJ Haas","year":"2013","journal-title":"Nat Protoc"},{"key":"pone.0343107.ref031","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: molecular evolutionary genetics analysis version 11","volume":"38","author":"K Tamura","year":"2021","journal-title":"Mol Biol Evol"},{"issue":"7873","key":"pone.0343107.ref032","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"J Jumper","year":"2021","journal-title":"Nature"},{"key":"pone.0343107.ref033","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1673\/031.010.14119","article-title":"Why does insect RNA look degraded?","volume":"10","author":"EC Winnebeck","year":"2010","journal-title":"J Insect Sci"},{"key":"pone.0343107.ref034","doi-asserted-by":"crossref","DOI":"10.7717\/peerj.1436","article-title":"\u201cDegraded\u201d RNA profiles in Arthropoda and beyond","volume":"3","author":"SD McCarthy","year":"2015","journal-title":"PeerJ"},{"issue":"1","key":"pone.0343107.ref035","doi-asserted-by":"crossref","DOI":"10.1093\/biomethods\/bpy012","article-title":"RNA profile diversity across arthropoda: guidelines, methodological artifacts, and expected 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Oliveira","year":"2015","journal-title":"Toxicon"},{"issue":"2","key":"pone.0343107.ref045","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0030478","article-title":"Identification and phylogenetic analysis of Tityus pachyurus and Tityus obscurus novel putative Na+-channel scorpion toxins","volume":"7","author":"JA Guerrero-Vargas","year":"2012","journal-title":"PLoS One"},{"issue":"6","key":"pone.0343107.ref046","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0128578","article-title":"The scorpion toxin Tf2 from Tityus fasciolatus promotes Nav1.3 opening","volume":"10","author":"TS Camargos","year":"2015","journal-title":"PLoS One"},{"key":"pone.0343107.ref047","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.toxicon.2020.06.011","article-title":"The three-dimensional structure of the toxic peptide Cl13 from the scorpion Centruroides limpidus","volume":"184","author":"AE 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Verano-Braga","year":"2010","journal-title":"Toxicon"},{"key":"pone.0343107.ref054","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.toxicon.2020.06.015","article-title":"Biology, venom composition, and scorpionism induced by brazilian scorpion Tityus stigmurus (Thorell, 1876) (Scorpiones: Buthidae): a mini-review","volume":"185","author":"AA Furtado","year":"2020","journal-title":"Toxicon"},{"key":"pone.0343107.ref055","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.toxicon.2015.04.003","article-title":"Homology modeling, vasorelaxant and bradykinin-potentiating activities of a novel hypotensin found in the scorpion venom from Tityus stigmurus","volume":"101","author":"RJA Machado","year":"2015","journal-title":"Toxicon"},{"key":"pone.0343107.ref056","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.toxicon.2016.06.002","article-title":"Characterization of TistH, a multifunctional peptide from the scorpion Tityus stigmurus: Structure, 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Naranjo","year":"1996","journal-title":"Neuron"},{"issue":"6","key":"pone.0343107.ref069","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/j.bcp.2008.07.008","article-title":"A common \u201chot spot\u201d confers hERG blockade activity to alpha-scorpion toxins affecting K+ channels","volume":"76","author":"Y Abdel-Mottaleb","year":"2008","journal-title":"Biochem Pharmacol"},{"key":"pone.0343107.ref070","first-page":"105102","article-title":"HADDOCK2.4: an integrative, multi-scale modelling platform for biomolecular complexes","volume":"152","author":"J Domanski","year":"2020","journal-title":"J Chem Phys"},{"issue":"2","key":"pone.0343107.ref071","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/nprot.2016.169","article-title":"The ClusPro web server for protein-protein docking","volume":"12","author":"D Kozakov","year":"2017","journal-title":"Nat Protoc"},{"key":"pone.0343107.ref072","first-page":"451","article-title":"On the evolution of invertebrate 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