{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:07:23Z","timestamp":1790186843891,"version":"4.1.0"},"reference-count":20,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["RF1NS28800"],"award-info":[{"award-number":["RF1NS28800"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NINDS T32 Pre-Doctoral Training Program in Movement Disorders and Neurorestoration","award":["5T32NS082168-09"],"award-info":[{"award-number":["5T32NS082168-09"]}]},{"name":"Training Grant on Alzheimer\u2019s Disease and ADRD at Indiana University","award":["5T32AG071444-05"],"award-info":[{"award-number":["5T32AG071444-05"]}],"id":[{"id":"https:\/\/ror.org\/01kg8sb98","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Stark Neuroscience Research Institute (SNRI) at Indiana University School of Medicine","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"https:\/\/ror.org\/02ets8c94","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Regulator of G-protein signaling 10 (RGS10) has been shown to regulate multiple inflammatory pathways relevant to disease pathogenesis. Of particular importance is the ability of RGS10 to negatively regulate the NFkB pathway, a prominent pro-inflammatory pathway implicated in multiple inflammatory disease phenotypes. However, the exact mechanism by which RGS10 regulates NF\u03baB is unknown. Given that RGS10 translocates to the nucleus upon stimulation, we hypothesize that RGS10 may regulate NF\u03baB at the transcript level. To determine whether RGS10 influences NF\u03baB transcript levels, we stimulated peritoneal macrophages from RGS10 Knockout (KO) and B6 mice and collected cell lysate and conditioned media over a 24-hour period to assess transcript levels of NF\u03baB and related pro-inflammatory cytokines as well as secreted cytokine levels. Here we found a limited and transient influence of RGS10 on transcript levels of NF\u03baB subunits and NF\u03baB-dependent cytokines. However, RGS10 displayed a more robust negative regulation of secreted protein levels of certain pro-inflammatory cytokines. Importantly, this study required the use of opioid analgesics prior to the collection of peritoneal macrophages and therefore reflects the role of RGS10 on pro-inflammatory transcript and protein levels when opioid analgesics are present. Overall, this study indicates that RGS10 may not serve as an important transcriptional regulator of NF\u03baB related cytokine production under the influence of opioid analgesics. Further studies are warranted to understand the influence of opioid analgesics on RGS10 function and the mechanism by which RGS10 regulates NF\u03baB activity.<\/jats:p>","DOI":"10.1371\/journal.pone.0343480","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:31:36Z","timestamp":1790184696000},"page":"e0343480","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["The limited role of RGS10 on NF\u03baB and cytokine transcript levels in peritoneal macrophages under the influence of opioid analgesics"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0771-384X","authenticated-orcid":true,"given":"Janna E.","family":"Jernigan Posey","sequence":"first","affiliation":[{"name":"University of Florida","place":["Gainesville, Florida, United States of America"],"department":["Center for Translational Research in Neurodegenerative Disease, College of Medicine"]},{"name":"University of Florida","place":["Gainesville, Florida, United States of America"],"department":["Department of Neuroscience, College of Medicine"]},{"name":"McKnight Brain Institute, University of Florida","place":["Gainesville, Florida, United States of America"]},{"name":"Indiana University School of Medicine","place":["Indianapolis, Indiana, United States of America"],"department":["Department of Neurology"]},{"name":"Stark Neuroscience Research Institute","place":["Indianapolis, Indiana, United States of America"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kruthika","family":"Dheeravath","sequence":"additional","affiliation":[{"name":"University of Florida","place":["Gainesville, Florida, United States of America"],"department":["Center for Translational Research in Neurodegenerative Disease, College of Medicine"]},{"name":"University of Florida","place":["Gainesville, Florida, United States of America"],"department":["Department 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