{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T06:19:40Z","timestamp":1773123580188,"version":"3.50.1"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:00:00Z","timestamp":1773014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004622","name":"KWF Kankerbestrijding","doi-asserted-by":"publisher","award":["11053\/2017-1"],"award-info":[{"award-number":["11053\/2017-1"]}],"id":[{"id":"10.13039\/501100004622","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["Veni 91615032"],"award-info":[{"award-number":["Veni 91615032"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Reprogramming of energy metabolism is one of the hallmarks of cancer cells and mutations that modify wild type intestinal cells to colon carcinomas increases cellular energy expenditure. Mitochondria are the main site for ATP production in (cancer) cells and disrupting their function results in impaired tumor forming efficacy. The mitochondrial ribosomal proteins (MRPs) constitute the ribosome specifically in mitochondria, and as such are crucial for the translation process of the electron transport chain complex subunits. We hence aimed to explore the consequence of reduced MRP expression on adenomagensis and investigate this in a genetic mouse model with bodywide heterozygosity for\n                    <jats:italic>Mrpl54<\/jats:italic>\n                    . We show that\n                    <jats:italic>Mrpl54<\/jats:italic>\n                    heterozygosity does not alter adenoma formation, intestinal proliferation or apoptosis in a heterozygous\n                    <jats:italic>Apc<\/jats:italic>\n                    model. Furthermore, diminished\n                    <jats:italic>Mrpl54<\/jats:italic>\n                    expression did not decrease stemness or global parameters of metabolism in colorectal cancer cell lines.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0344161","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T17:27:05Z","timestamp":1773077225000},"page":"e0344161","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reduced levels of mitochondrial ribosomal protein MRPL54 does not alter Apc related adenoma formation"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7557-5827","authenticated-orcid":true,"given":"Claudia N.","family":"Spaan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eileen","family":"Daniels","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wouter L.","family":"Smit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruben J.","family":"de Boer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joana","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline L. M.","family":"Vermeulen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Meisner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanesa","family":"Muncan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riekelt H.","family":"Houtkooper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jarom","family":"Heijmans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,3,9]]},"reference":[{"issue":"1","key":"pone.0344161.ref001","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1158\/2159-8290.CD-21-1059","article-title":"Hallmarks of cancer: New dimensions","volume":"12","author":"D Hanahan","year":"2022","journal-title":"Cancer Discov"},{"issue":"6","key":"pone.0344161.ref002","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1085\/jgp.8.6.519","article-title":"The metabolism of tumors in the body","volume":"8","author":"O Warburg","year":"1927","journal-title":"J Gen Physiol"},{"issue":"5947","key":"pone.0344161.ref003","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1126\/science.1174229","article-title":"Glucose deprivation contributes to the development of KRAS pathway mutations in tumor cells","volume":"325","author":"J Yun","year":"2009","journal-title":"Science"},{"issue":"11","key":"pone.0344161.ref004","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1038\/nm.3967","article-title":"The consensus molecular subtypes of colorectal cancer","volume":"21","author":"J Guinney","year":"2015","journal-title":"Nat Med"},{"issue":"11","key":"pone.0344161.ref005","doi-asserted-by":"crossref","first-page":"3535","DOI":"10.1073\/pnas.82.11.3535","article-title":"Glycolysis and methylaminoisobutyrate uptake in rat-1 cells transfected with ras or myc oncogenes","volume":"82","author":"E Racker","year":"1985","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"pone.0344161.ref006","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/j.cell.2012.01.058","article-title":"Oncogenic Kras maintains pancreatic tumors through regulation of anabolic glucose metabolism","volume":"149","author":"H Ying","year":"2012","journal-title":"Cell"},{"issue":"11","key":"pone.0344161.ref007","doi-asserted-by":"crossref","DOI":"10.26508\/lsa.202301912","article-title":"Grp78 is required for intestinal Kras-dependent glycolysis proliferation and adenomagenesis","volume":"6","author":"CN Spaan","year":"2023","journal-title":"Life Sci Alliance"},{"issue":"21","key":"pone.0344161.ref008","doi-asserted-by":"crossref","first-page":"6098","DOI":"10.1158\/0008-5472.CAN-17-3600","article-title":"Heterozygosity of Chaperone Grp78 Reduces Intestinal Stem Cell Regeneration Potential and Protects against Adenoma Formation","volume":"78","author":"JF van Lidth de Jeude","year":"2018","journal-title":"Cancer Res"},{"issue":"6","key":"pone.0344161.ref009","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/j.beem.2012.05.003","article-title":"Cellular and molecular mechanisms of mitochondrial function","volume":"26","author":"LD Osellame","year":"2012","journal-title":"Best Pract Res Clin Endocrinol Metab"},{"issue":"7","key":"pone.0344161.ref010","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.1016\/j.celrep.2018.01.066","article-title":"Kinetics and mechanism of mammalian mitochondrial ribosome assembly","volume":"22","author":"DF Bogenhagen","year":"2018","journal-title":"Cell Rep"},{"issue":"5","key":"pone.0344161.ref011","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1016\/j.molcel.2016.02.011","article-title":"Mitochondria and cancer","volume":"61","author":"WX Zong","year":"2016","journal-title":"Mol Cell"},{"issue":"11","key":"pone.0344161.ref012","first-page":"1189","article-title":"Diminished tumorigenic phenotype after depletion of mitochondrial DNA","volume":"8","author":"LR Cavalli","year":"1997","journal-title":"Cell Growth Differ"},{"issue":"14","key":"pone.0344161.ref013","first-page":"3889","article-title":"Tumor-forming ability in athymic nude mice of human cell lines devoid of mitochondrial DNA","volume":"54","author":"R Morais","year":"1994","journal-title":"Cancer Res"},{"issue":"1","key":"pone.0344161.ref014","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.cmet.2014.12.003","article-title":"Mitochondrial genome acquisition restores respiratory function and tumorigenic potential of cancer cells without mitochondrial DNA","volume":"21","author":"AS Tan","year":"2015","journal-title":"Cell Metab"},{"issue":"1","key":"pone.0344161.ref015","doi-asserted-by":"crossref","first-page":"10658","DOI":"10.1038\/s41598-022-14724-7","article-title":"Expression and prognosis analysis of mitochondrial ribosomal protein family in breast cancer","volume":"12","author":"X Lin","year":"2022","journal-title":"Sci Rep"},{"key":"pone.0344161.ref016","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.semcancer.2017.04.004","article-title":"Mitochondrial ribosomes in cancer","volume":"47","author":"H-J Kim","year":"2017","journal-title":"Semin Cancer Biol"},{"issue":"1","key":"pone.0344161.ref017","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1186\/s12890-023-02677-0","article-title":"Knockdown of MRPL35 promotes cell apoptosis and inhibits cell proliferation in non-small-cell lung cancer","volume":"23","author":"C Zhao","year":"2023","journal-title":"BMC Pulm Med"},{"issue":"7","key":"pone.0344161.ref018","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1016\/j.cell.2005.04.015","article-title":"Structural basis for the function of the ribosomal L7\/12 stalk in factor binding and GTPase activation","volume":"121","author":"M Diaconu","year":"2005","journal-title":"Cell"},{"key":"pone.0344161.ref019","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.58362","article-title":"Structural basis of mitochondrial translation","volume":"9","author":"S Aibara","year":"2020","journal-title":"Elife"},{"issue":"6","key":"pone.0344161.ref020","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/j.cmet.2016.08.017","article-title":"A Genome-wide CRISPR death screen identifies genes essential for oxidative phosphorylation","volume":"24","author":"JD Arroyo","year":"2016","journal-title":"Cell Metab"},{"issue":"1","key":"pone.0344161.ref021","doi-asserted-by":"crossref","first-page":"8391","DOI":"10.1038\/s41598-023-35196-3","article-title":"Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in mice","volume":"13","author":"K Reid","year":"2023","journal-title":"Sci Rep"},{"issue":"7450","key":"pone.0344161.ref022","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1038\/nature12188","article-title":"Mitonuclear protein imbalance as a conserved longevity mechanism","volume":"497","author":"RH Houtkooper","year":"2013","journal-title":"Nature"},{"issue":"5335","key":"pone.0344161.ref023","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1126\/science.278.5335.120","article-title":"Rapid colorectal adenoma formation initiated by conditional targeting of the Apc gene","volume":"278","author":"H Shibata","year":"1997","journal-title":"Science"},{"issue":"1","key":"pone.0344161.ref024","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1038\/5007","article-title":"Generalized lacZ expression with the ROSA26 Cre reporter strain","volume":"21","author":"P Soriano","year":"1999","journal-title":"Nat Genet"},{"issue":"3","key":"pone.0344161.ref025","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1002\/gene.20042","article-title":"Tissue-specific and inducible Cre-mediated recombination in the gut epithelium","volume":"39","author":"F el Marjou","year":"2004","journal-title":"Genesis"},{"issue":"12","key":"pone.0344161.ref026","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1101\/gad.287404","article-title":"Loss of Apc in vivo immediately perturbs Wnt signaling, differentiation, and migration","volume":"18","author":"OJ Sansom","year":"2004","journal-title":"Genes Dev"},{"issue":"1","key":"pone.0344161.ref027","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1038\/nn.2467","article-title":"A robust and high-throughput Cre reporting and characterization system for the whole mouse brain","volume":"13","author":"L Madisen","year":"2010","journal-title":"Nat Neurosci"},{"issue":"6","key":"pone.0344161.ref028","doi-asserted-by":"crossref","DOI":"10.1371\/annotation\/b60d4ec5-4c6f-43ab-9f63-322e3cd59636","article-title":"Correction: Intestinal tumorigenesis is not affected by progesterone signaling in rodent models","volume":"8","author":"J Heijmans","year":"2013","journal-title":"PLoS One"},{"issue":"7","key":"pone.0344161.ref029","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0022620","article-title":"Intestinal tumorigenesis is not affected by progesterone signaling in rodent models","volume":"6","author":"J Heijmans","year":"2011","journal-title":"PLoS One"},{"issue":"4","key":"pone.0344161.ref030","doi-asserted-by":"crossref","first-page":"148367","DOI":"10.1016\/j.bbabio.2020.148367","article-title":"Soluble adenylyl cyclase regulates the cytosolic NADH\/NAD+ redox state and the bioenergetic switch between glycolysis and oxidative phosphorylation","volume":"1862","author":"J-C Chang","year":"2021","journal-title":"Biochim Biophys Acta Bioenerg"},{"issue":"41","key":"pone.0344161.ref031","doi-asserted-by":"crossref","first-page":"25560","DOI":"10.1073\/pnas.1912772117","article-title":"Driver mutations of the adenoma-carcinoma sequence govern the intestinal epithelial global translational capacity","volume":"117","author":"WL Smit","year":"2020","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"pone.0344161.ref032","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/0092-8674(90)90186-I","article-title":"A genetic model for colorectal tumorigenesis","volume":"61","author":"ER Fearon","year":"1990","journal-title":"Cell"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0344161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T17:27:13Z","timestamp":1773077233000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0344161"}},"subtitle":[],"editor":[{"given":"Irina V.","family":"Lebedeva","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,3,9]]},"references-count":32,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,3,9]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0344161","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,9]]}}}