{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:07:31Z","timestamp":1773878851794,"version":"3.50.1"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012752","name":"Elanco Animal Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012752","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Narasin is an ionophore commonly used to improve energy efficiency and feed utilization in beef cattle. This study evaluated the impact of increasing levels of narasin on performance, feeding behavior, carcass traits, and ruminal and cecum morphometrics of Nellore cattle in feedlot. One hundred twenty-eight Nellore yearling bulls (393\u2009\u00b1\u200924\u2009kg) were blocked by initial body weight (BW), allocated in 32 pens (4 bulls\/pen). Pens were randomly assigned to one of four treatments: 0 (control), 13, 20, or 27\u2009ppm of narasin. After a 14-day adaptation, animals were fed a high-concentrate diet (87% concentrate, dry matter basis) for 98 days. Narasin was not included in the diet from day 109 to day 111 due to the withdrawal period required by the active compound. Animals were slaughtered on day 112. A significant linear effect of narasin was observed on BW (P\u2009=\u20090.02) and average daily gain (P\u2009=\u20090.01) during the first 28 days, with improved feed efficiency at 20-ppm (P\u2009=\u20090.03). These effects did not persist over the full 111 d period. Cattle receiving 20-ppm of narasin had longer meal durations on day 70 (P\u2009=\u20090.05). A quadratic effect was observed on the thickness of fat at the 12th rib (P\u2009=\u20090.05), with the highest mean value observed at 20\u2009ppm. The 13-ppm level increased the rumen papillae size (P\u2009=\u20090.02), while higher levels reduced the number of goblet cells in the cecum (P\u2009=\u20090.01). Narasin supplementation enhanced early feedlot performance and carcass fat deposition without negatively affecting dry matter intake. Narasin can be used in feedlot diets from 13 to 27\u2009ppm, with 20\u2009ppm being the most effective level for improving performance and carcass quality.<\/jats:p>","DOI":"10.1371\/journal.pone.0344240","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T17:30:54Z","timestamp":1773855054000},"page":"e0344240","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of increasing narasin doses on feedlot performance, feeding behavior, carcass traits, and rumen-cecum morphometrics in Nellore cattle"],"prefix":"10.1371","volume":"21","author":[{"given":"Leandro Aparecido","family":"Ferreira da Silva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Moretto","family":"Casali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Laura 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J\u00fanior","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johnny Maciel","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1253-7310","authenticated-orcid":true,"given":"Danilo Domingues","family":"Millen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"pone.0344240.ref001","doi-asserted-by":"crossref","DOI":"10.37496\/rbz5020200189","article-title":"The 2019 Brazilian survey on nutritional practices provided by feedlot cattle consulting nutritionists","volume":"50","author":"AM Silvestre","year":"2021","journal-title":"Rev Bras Zootec"},{"key":"pone.0344240.ref002","article-title":"Adaptation of beef cattle to high-concentrate diets: performance and ruminal metabolism","author":"MS 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