{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T18:13:23Z","timestamp":1788372803955,"version":"build-2803163510"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI13\/01729"],"award-info":[{"award-number":["PI13\/01729"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI16\/00174"],"award-info":[{"award-number":["PI16\/00174"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI19\/00104"],"award-info":[{"award-number":["PI19\/00104"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI23\/00049"],"award-info":[{"award-number":["PI23\/00049"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"PFIS fellowship","award":["FI24\/00206"],"award-info":[{"award-number":["FI24\/00206"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Respiratory tract infections range from asymptomatic colonisation to an invasive disease. Recent studies suggest that nasopharyngeal microbiota may influence this variability. Emerging evidence points to\n                      <jats:italic>Dolosigranulum pigrum,<\/jats:italic>\n                      a nasopharyngeal commensal, as a potentially protective bacterium. This study aimed to identify variables associated with the presence of\n                      <jats:italic>D. pigrum<\/jats:italic>\n                      in the nasopharynx of children with varying respiratory health statuses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Nasopharyngeal aspirates were collected from children &lt;18 years who were asymptomatic (n\u2009=\u200965), had banal viral infection (n\u2009=\u200948), or Invasive Pneumococcal Disease (IPD) (n\u2009=\u200927). The presence of\n                      <jats:italic>D. pigrum<\/jats:italic>\n                      was defined as &gt;0.1% of total sequences obtained by 16S rRNA gene sequencing. Variables included sex, breastfeeding, delivery mode,\n                      <jats:italic>S. pneumoniae<\/jats:italic>\n                      carriage, respiratory viruses and clinical features.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among 140 children (73 males, 67 females),\n                      <jats:italic>D. pigrum<\/jats:italic>\n                      was detected in 79 (56.4%): 44\/65 in the healthy group; 26\/48 of viral and 9\/27 IPD cases. Multivariate analysis revealed significant associations with health status and sex. Healthy children were more likely to carry\n                      <jats:italic>D. pigrum<\/jats:italic>\n                      than IPD cases (44\/79 vs. 26\/79; p\u2009=\u20090.028). Males were more frequently\n                      <jats:italic>D. pigrum<\/jats:italic>\n                      carriers than females (48\/79\n                      <jats:italic>vs.<\/jats:italic>\n                      31\/79; p\u2009=\u20090.033).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>D. Pigrum<\/jats:italic>\n                      was associated with respiratory health, being more prevalent in healthy children, and showed potential sex-related differences.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0345189","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T17:22:29Z","timestamp":1788369749000},"page":"e0345189","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Presence of Dolosigranulum pigrum in the nasopharynx and its relationship with respiratory health status in paediatric population"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9715-887X","authenticated-orcid":true,"given":"Maria","family":"Cisneros","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desir\u00e9e","family":"Henares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleix","family":"Lluans\u00ed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Brotons","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristian","family":"Launes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Penela-Sanchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Gonzalez-Comino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amaresh","family":"Perez-Arguello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariona 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