{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T18:44:59Z","timestamp":1774982699572,"version":"3.50.1"},"reference-count":26,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Health, Republic of Korea","award":["2024ER051701"],"award-info":[{"award-number":["2024ER051701"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>This study presents a nationwide infrastructure for the collection and utilization of gynecologic cancer biospecimens, established through the Korea Biobank Project. We comprehensively describe the biobanking strategy, quality control protocols, and development of secondary resources to support future translational and discovery-based research.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We established a gynecologic cancer biobank within the Korea Biobank Project (KBP) through a multi-institutional consortium. Biospecimens, including blood, tumor tissue, urine, and ascites, were collected from 294 patients with endometrial, cervical, or ovarian cancers. Pre-analytical variables were documented using the Standard PREanalytical Code (SPREC), and all samples were tracked with 2D barcodes. Secondary resources were developed, including whole-genome sequencing (WGS) datasets, immortalized human ovarian surface epithelial (IHOSE) cell lines, patient-derived xenografts (PDX), tumor organoids, and tissue microarrays (TMAs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 6,168 biospecimens were archived. WGS was performed on 386 cancer samples, including 172 paired tumor\u2013normal sets. Four IHOSE cell lines were authenticated and validated for stability, 14 PDX models retained histological fidelity across passages, and patient-derived ovarian cancer organoids demonstrated drug sensitivity consistent with clinical response patterns. TMAs were constructed from 519 tumors, supporting large-scale molecular profiling. Industry collaborations further highlighted the translational utility of these resources.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study describes the development and application of a gynecologic cancer biobank that integrates standardized biospecimen collection, rigorous QC, and the generation of diverse secondary resources. By linking these resources with clinical and epidemiological data, the biobank provides a scalable and accessible platform for precision oncology and academic\u2013industry collaboration.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0345861","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T17:45:36Z","timestamp":1774979136000},"page":"e0345861","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biobanking of gynecologic cancer biospecimens: Development, quality control, and translational applications"],"prefix":"10.1371","volume":"21","author":[{"given":"Jue Young","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoo-Kyung","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ha-Yeon","family":"Shin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoon Joo","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi Ae","family":"Jeon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wookyeom","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Jun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyungmin","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanbyoul","family":"Cho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-A","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6599-7065","authenticated-orcid":true,"given":"Jae-Hoon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,3,31]]},"reference":[{"key":"pone.0345861.ref001","first-page":"362","article-title":"Development of a Big Data Platform for the Collection and Utilization of Korea Biobank Network Clinical Information","volume":"316","author":"YS Im","year":"2024","journal-title":"Stud Health Technol Inform"},{"key":"pone.0345861.ref002","doi-asserted-by":"crossref","first-page":"7769","DOI":"10.1038\/ncomms8769","article-title":"PI3K\/AKT activation induces PTEN ubiquitination and destabilization accelerating tumourigenesis","volume":"6","author":"M-S Lee","year":"2015","journal-title":"Nat Commun"},{"issue":"9","key":"pone.0345861.ref003","doi-asserted-by":"crossref","first-page":"4098","DOI":"10.1172\/JCI96804","article-title":"Mitochondrial reprogramming via ATP5H loss promotes multimodal cancer therapy resistance","volume":"128","author":"K-H Song","year":"2018","journal-title":"J Clin Invest"},{"issue":"4","key":"pone.0345861.ref004","article-title":"Exploring the characteristics of immortalized human ovarian surface epithelial cell lines","volume":"11","author":"H-Y Shin","year":"2025","journal-title":"Heliyon"},{"issue":"2","key":"pone.0345861.ref005","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1002\/cjp2.305","article-title":"Real-life data from standardized preanalytical coding (SPREC) in tissue biobanking and its dual use for sample characterization and process optimization","volume":"9","author":"M Skoworonska","year":"2023","journal-title":"J Pathol Clin Res"},{"issue":"3","key":"pone.0345861.ref006","doi-asserted-by":"crossref","first-page":"829","DOI":"10.3390\/cancers14030829","article-title":"Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models","volume":"14","author":"H-Y Shin","year":"2022","journal-title":"Cancers (Basel)"},{"issue":"8","key":"pone.0345861.ref007","doi-asserted-by":"crossref","first-page":"1856","DOI":"10.1038\/s12276-024-01288-x","article-title":"Clinical application of whole-genome sequencing of solid tumors for precision oncology","volume":"56","author":"R Kim","year":"2024","journal-title":"Exp Mol Med"},{"issue":"4","key":"pone.0345861.ref008","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1038\/nm.4292","article-title":"HRDetect is a predictor of BRCA1 and BRCA2 deficiency based on mutational signatures","volume":"23","author":"H Davies","year":"2017","journal-title":"Nat Med"},{"issue":"11","key":"pone.0345861.ref009","article-title":"RNA sequencing and bioinformatics analysis revealed PACSIN3 as a potential novel biomarker for platinum resistance in epithelial ovarian cancer","volume":"24","author":"GH Han","year":"2022","journal-title":"J Gene Med"},{"issue":"6","key":"pone.0345861.ref010","doi-asserted-by":"crossref","first-page":"692","DOI":"10.21873\/cgp.20352","article-title":"TMED9 Expression Level as a Biomarker of Epithelial Ovarian Cancer Progression and Prognosis","volume":"19","author":"GH Han","year":"2022","journal-title":"Cancer Genomics Proteomics"},{"issue":"5","key":"pone.0345861.ref011","doi-asserted-by":"crossref","first-page":"102","DOI":"10.3892\/or.2023.8539","article-title":"High ATP6V1B1 expression is associated with poor prognosis and platinum\u2011based chemotherapy resistance in epithelial ovarian cancer","volume":"49","author":"GH Han","year":"2023","journal-title":"Oncol Rep"},{"issue":"1","key":"pone.0345861.ref012","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1038\/s42003-023-05166-6","article-title":"Skp2-mediated MLKL degradation confers cisplatin-resistant in non-small cell lung cancer cells","volume":"6","author":"H Zhou","year":"2023","journal-title":"Commun Biol"},{"issue":"2","key":"pone.0345861.ref013","first-page":"97","article-title":"In vitro assessment of BBI608 in 2D and 3D culture models for drug repositioning in oral squamous cell carcinoma","volume":"54","author":"D-G Park","year":"2025","journal-title":"Oncol Rep"},{"issue":"1","key":"pone.0345861.ref014","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1186\/s40164-025-00681-0","article-title":"Mitotic MTH1 inhibitor karonudib kills epithelial ovarian cancer independent of platinum sensitivity","volume":"14","author":"RM Hurley","year":"2025","journal-title":"Exp Hematol Oncol"},{"issue":"1","key":"pone.0345861.ref015","doi-asserted-by":"crossref","first-page":"12581","DOI":"10.1038\/s41598-020-69488-9","article-title":"Patient-derived ovarian cancer organoids capture the genomic profiles of primary tumours applicable for drug sensitivity and resistance testing","volume":"10","author":"Y Nanki","year":"2020","journal-title":"Sci Rep"},{"issue":"5","key":"pone.0345861.ref016","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1038\/s41591-019-0422-6","article-title":"An organoid platform for ovarian cancer captures intra- and interpatient heterogeneity","volume":"25","author":"O Kopper","year":"2019","journal-title":"Nat Med"},{"issue":"11","key":"pone.0345861.ref017","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1158\/2159-8290.CD-18-0474","article-title":"Prediction of DNA Repair Inhibitor Response in Short-Term Patient-Derived Ovarian Cancer Organoids","volume":"8","author":"SJ Hill","year":"2018","journal-title":"Cancer Discov"},{"issue":"5","key":"pone.0345861.ref018","doi-asserted-by":"crossref","first-page":"101520","DOI":"10.1016\/j.gendis.2025.101520","article-title":"Patient-derived xenograft models: Current status, challenges, and innovations in cancer research","volume":"12","author":"M Liu","year":"2025","journal-title":"Genes Dis"},{"issue":"1","key":"pone.0345861.ref019","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1186\/s12967-025-06787-7","article-title":"Metabolic, transcriptomic, and proteomic adaptations in pancreatic ductal adenocarcinoma-patient derived xenograft models across serial passages","volume":"23","author":"P Guo","year":"2025","journal-title":"J Transl Med"},{"issue":"2","key":"pone.0345861.ref020","article-title":"Cohort profile: design and methods in the eye and vision consortium of UK Biobank","volume":"9","author":"SYL Chua","year":"2019","journal-title":"BMJ Open"},{"issue":"3","key":"pone.0345861.ref021","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1093\/jamia\/ocab260","article-title":"Curating a longitudinal research resource using linked primary care EHR data-a UK Biobank case study","volume":"29","author":"P Darke","year":"2022","journal-title":"J Am Med Inform Assoc"},{"key":"pone.0345861.ref022","doi-asserted-by":"crossref","DOI":"10.1016\/j.je.2016.12.005","article-title":"Overview of the BioBank Japan Project: Study design and profile","volume":"27","author":"A Nagai","year":"2017","journal-title":"J Epidemiol"},{"issue":"1","key":"pone.0345861.ref023","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1186\/s12911-025-03192-4","article-title":"Development of a big data platform for collecting and utilizing clinical information from the Korea Biobank Network","volume":"25","author":"YS Im","year":"2025","journal-title":"BMC Med Inform Decis Mak"},{"issue":"1","key":"pone.0345861.ref024","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1186\/s13058-025-02063-0","article-title":"Anti-cancer drug sensitivity testing and preclinical evaluation of the anti-cancer potential of WEE1 inhibitor in triple-negative breast cancer patient-derived organoids and xenograft models","volume":"27","author":"S Ryu","year":"2025","journal-title":"Breast Cancer Res"},{"issue":"1","key":"pone.0345861.ref025","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/s41419-023-05566-w","article-title":"Single-cell RNA-seq integrated with multi-omics reveals SERPINE2 as a target for metastasis in advanced renal cell carcinoma","volume":"14","author":"W-J Chen","year":"2023","journal-title":"Cell Death Dis"},{"issue":"1","key":"pone.0345861.ref026","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1038\/s41540-024-00462-1","article-title":"Microarray integrated spatial transcriptomics (MIST) for affordable and robust digital pathology","volume":"10","author":"Juwayria","year":"2024","journal-title":"NPJ Syst Biol Appl"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0345861","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T17:45:43Z","timestamp":1774979143000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0345861"}},"subtitle":[],"editor":[{"given":"Kazunori","family":"Nagasaka","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,3,31]]},"references-count":26,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,3,31]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0345861","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,31]]}}}