{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:44:13Z","timestamp":1792291453174,"version":"4.4.0"},"reference-count":131,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100023448","name":"Robert Koch Institut","doi-asserted-by":"publisher","award":["NRZ Borrelia"],"award-info":[{"award-number":["NRZ Borrelia"]}],"id":[{"id":"10.13039\/501100023448","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Lyme borreliosis is caused by species of the\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    sensu lato complex. Genospecies vary in human pathogenicity, reservoir host and tick vector adaptation. What causes these differences is currently unknown, but some answers are likely to be found within the fragmented genomes containing linear and circular plasmids (and even fusions). These plasmids are highly variable and challenging to reconstruct completely. We aimed to analyze plasmid presence\/absence and to identify trends in plasmid numbers and\/or types associated with specific ecological adaptations or pathogenic potentials. For this, we used the most complete, reliable, and currently available 86\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    s.l. genomes (30 newly sequenced, 56 publicly available) belonging to 21 of the 28 known species. The plasmid types per isolate vary from 6-21 (median: 12) and linear plasmid numbers (3\u201312, median: 7) are significantly higher than circular plasmids (2\u201311, median: 5). The only plasmids found in all isolates are lp54 and cp26 and only a few species- or population-specific plasmids are identified, demonstrating the high variability. Species with smaller plasmid counts (median\u2009&lt;\u200911) tend to have fewer lp28 and cp32 plasmids than larger plasmid counts species (median\u2009\u2265\u200915). Significantly higher total and circular plasmid numbers are found in rodents-only compared to birds-only associated species. Interestingly, plasmid numbers are not significantly reduced in species associated with a single reservoir host\/vector compared to multiple. Analyses of the plasmid-location of the\n                    <jats:italic>vls\/vlsE<\/jats:italic>\n                    antigenic variation system highlighted the dynamic nature of\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    s.l. plasmids. Surprisingly, whilst the plasmid repertoire of\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    s.l. is very plastic and variable, our analyses rarely reveal plasmids associated with specific ecological adaptations or pathogenic potentials. However, some plasmids were more frequently found in one of the groups and will be of interest for gene-based analyses that will be required for further progress in this research area.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0346097","type":"journal-article","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T23:12:29Z","timestamp":1778281949000},"page":"e0346097","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["The wild, wild west of plasmids: First insights into comparative genomics of Borrelia burgdorferi sensu lato"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7768-4867","authenticated-orcid":true,"given":"Sabrina","family":"Hepner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5779-7001","authenticated-orcid":true,"given":"Robert E.","family":"Rollins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7411-4743","authenticated-orcid":true,"given":"Evangelos","family":"Mourkas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin","family":"Kuleshov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherwood","family":"Casjens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weigang","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Dangel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dunia","family":"Vel\u00e1zquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4669-5288","authenticated-orcid":true,"given":"Sergey Y.","family":"Kovalev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Wieser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"H\u00fcbner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Sing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"Fingerle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Margos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,5,8]]},"reference":[{"issue":"9814","key":"pone.0346097.ref001","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/S0140-6736(11)60103-7","article-title":"Lyme borreliosis","volume":"379","author":"G Stanek","year":"2012","journal-title":"Lancet"},{"key":"pone.0346097.ref002","first-page":"1","article-title":"Borrelia ecology, evolution, and human disease: a mosaic of life.","author":"G Margos","year":"2023","journal-title":"Zoonoses: infections affecting humans and animals. 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Margos","year":"2017","journal-title":"BMC Genomics"},{"issue":"4","key":"pone.0346097.ref027","first-page":"401","article-title":"Borrelia genomes in the year 2000","volume":"2","author":"S Casjens","year":"2000","journal-title":"J Mol Microbiol Biotechnol"},{"issue":"3","key":"pone.0346097.ref028","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0033280","article-title":"Genome stability of Lyme disease spirochetes: comparative genomics of Borrelia burgdorferi plasmids","volume":"7","author":"SR Casjens","year":"2012","journal-title":"PLoS One"},{"key":"pone.0346097.ref029","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1186\/1471-2164-14-693","article-title":"Inter- and intra-specific pan-genomes of Borrelia burgdorferi sensu lato: genome stability and adaptive radiation","volume":"14","author":"EF Mongodin","year":"2013","journal-title":"BMC 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burgdorferi BBK32 provides insight into classical pathway complement evasion by Lyme disease spirochetes","volume":"15","author":"J Xie","year":"2019","journal-title":"PLoS Pathog"},{"issue":"7","key":"pone.0346097.ref068","doi-asserted-by":"crossref","DOI":"10.1111\/cmi.13029","article-title":"A small intergenic region of lp17 is required for evasion of adaptive immunity and induction of pathology by the Lyme disease spirochete","volume":"21","author":"T Casselli","year":"2019","journal-title":"Cell Microbiol"},{"issue":"12","key":"pone.0346097.ref069","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0168994","article-title":"Whole genome sequence and comparative genomics of the novel Lyme borreliosis causing pathogen, Borrelia mayonii","volume":"11","author":"LC Kingry","year":"2016","journal-title":"PLoS One"},{"issue":"4","key":"pone.0346097.ref070","doi-asserted-by":"crossref","DOI":"10.1128\/genomeA.00655-16","article-title":"Chromosome and linear plasmid sequences of a 2015 human isolate of the tick-borne relapsing fever spirochete, Borrelia turicatae","volume":"4","author":"LC Kingry","year":"2016","journal-title":"Genome Announc"},{"issue":"1","key":"pone.0346097.ref071","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1111\/mmi.13873","article-title":"Analysis of recombinational switching at the antigenic variation locus of the Lyme spirochete using a novel PacBio sequencing pipeline","volume":"107","author":"TB Verhey","year":"2018","journal-title":"Mol Microbiol"},{"issue":"9","key":"pone.0346097.ref072","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.01749-24","article-title":"Natural selection and recombination at host-interacting lipoprotein loci drive genome diversification of Lyme disease and related bacteria","volume":"15","author":"S Akther","year":"2024","journal-title":"mBio"},{"issue":"1","key":"pone.0346097.ref073","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1186\/s12864-023-09500-4","article-title":"A high fidelity approach to assembling the complex Borrelia genome","volume":"24","author":"S Hepner","year":"2023","journal-title":"BMC Genomics"},{"issue":"8","key":"pone.0346097.ref074","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1011243","article-title":"Whole genome sequencing of human Borrelia burgdorferi isolates reveals linked blocks of accessory genome elements located on plasmids and associated with human dissemination","volume":"19","author":"JE Lemieux","year":"2023","journal-title":"PLoS Pathog"},{"key":"pone.0346097.ref075","doi-asserted-by":"crossref","DOI":"10.1093\/infdis\/jiae256","article-title":"Analysis of the borreliaceae pangenome reveals a distinct genomic architecture conserved across phylogenetic scales","volume":"230","author":"JE Lemieux","year":"2024","journal-title":"J Infect Dis"},{"issue":"4","key":"pone.0346097.ref076","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.ijmm.2007.01.006","article-title":"Multilocus sequence analysis of atypical Borrelia burgdorferi sensu lato isolates--description of Borrelia californiensis sp. nov., and genomospecies 1 and 2","volume":"297","author":"D Postic","year":"2007","journal-title":"Int J Med Microbiol"},{"issue":"16","key":"pone.0346097.ref077","doi-asserted-by":"crossref","first-page":"5410","DOI":"10.1128\/AEM.00116-09","article-title":"A new Borrelia species defined by multilocus sequence analysis of housekeeping genes","volume":"75","author":"G Margos","year":"2009","journal-title":"Appl Environ Microbiol"},{"issue":"9","key":"pone.0346097.ref078","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1101\/gr.263566.120","article-title":"HiCanu: accurate assembly of segmental duplications, satellites, and allelic variants from high-fidelity long reads","volume":"30","author":"S Nurk","year":"2020","journal-title":"Genome Res"},{"issue":"5","key":"pone.0346097.ref079","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"A Bankevich","year":"2012","journal-title":"J Comput Biol"},{"issue":"7","key":"pone.0346097.ref080","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1093\/bioinformatics\/btv688","article-title":"hybridSPAdes: an algorithm for hybrid assembly of short and long reads","volume":"32","author":"D Antipov","year":"2016","journal-title":"Bioinformatics"},{"issue":"3","key":"pone.0346097.ref081","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1046\/j.1365-2958.2000.01698.x","article-title":"A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi","volume":"35","author":"S Casjens","year":"2000","journal-title":"Mol Microbiol"},{"issue":"3","key":"pone.0346097.ref082","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1046\/j.1365-2958.1997.6051963.x","article-title":"Telomeres of the linear chromosomes of Lyme disease spirochaetes: nucleotide sequence and possible exchange with linear plasmid telomeres","volume":"26","author":"S Casjens","year":"1997","journal-title":"Mol Microbiol"},{"issue":"3","key":"pone.0346097.ref083","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"SF Altschul","year":"1990","journal-title":"J Mol Biol"},{"issue":"12","key":"pone.0346097.ref084","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0208432","article-title":"The genus Borrelia reloaded","volume":"13","author":"G Margos","year":"2018","journal-title":"PLoS One"},{"issue":"28","key":"pone.0346097.ref085","doi-asserted-by":"crossref","DOI":"10.1128\/genomeA.00637-17","article-title":"Completed genome sequences of Borrelia burgdorferi sensu stricto B31(NRZ) and closely related patient isolates from Europe","volume":"5","author":"G Margos","year":"2017","journal-title":"Genome Announc"},{"issue":"11","key":"pone.0346097.ref086","doi-asserted-by":"crossref","first-page":"7264","DOI":"10.1074\/jbc.M808918200","article-title":"Characterization and in vitro reaction properties of 19 unique hairpin telomeres from the linear plasmids of the Lyme disease spirochete","volume":"284","author":"Y Tourand","year":"2009","journal-title":"J Biol Chem"},{"issue":"13","key":"pone.0346097.ref087","doi-asserted-by":"crossref","first-page":"4134","DOI":"10.1128\/JB.186.13.4134-4141.2004","article-title":"Telomere exchange between linear replicons of Borrelia burgdorferi","volume":"186","author":"WM Huang","year":"2004","journal-title":"J Bacteriol"},{"issue":"22","key":"pone.0346097.ref088","doi-asserted-by":"crossref","first-page":"7233","DOI":"10.1128\/jb.173.22.7233-7239.1991","article-title":"Linear plasmids of Borrelia burgdorferi have a telomeric structure and sequence similar to those of a eukaryotic virus","volume":"173","author":"J Hinnebusch","year":"1991","journal-title":"J Bacteriol"},{"key":"pone.0346097.ref089","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1186\/1471-2105-10-421","article-title":"BLAST+: architecture and applications","volume":"10","author":"C Camacho","year":"2009","journal-title":"BMC Bioinform"},{"issue":"1","key":"pone.0346097.ref090","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1186\/s12864-016-3016-4","article-title":"Recurrent evolution of host and vector association in bacteria of the Borrelia burgdorferi sensu lato species complex","volume":"17","author":"NS Becker","year":"2016","journal-title":"BMC Genomics"},{"issue":"2","key":"pone.0346097.ref091","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1128\/jcm.31.2.340-350.1993","article-title":"An OspA serotyping system for Borrelia burgdorferi based on reactivity with monoclonal antibodies and OspA sequence analysis","volume":"31","author":"B Wilske","year":"1993","journal-title":"J Clin Microbiol"},{"issue":"1","key":"pone.0346097.ref092","doi-asserted-by":"crossref","first-page":"100935","DOI":"10.1016\/j.crmeth.2024.100935","article-title":"A core genome MLST scheme for Borrelia burgdorferi sensu lato improves insights into the evolutionary history of the species complex","volume":"5","author":"S Hepner","year":"2025","journal-title":"Cell Rep Methods"},{"issue":"4","key":"pone.0346097.ref093","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT multiple sequence alignment software version 7: improvements in performance and usability","volume":"30","author":"K Katoh","year":"2013","journal-title":"Mol Biol Evol"},{"key":"pone.0346097.ref094","doi-asserted-by":"crossref","first-page":"124","DOI":"10.12688\/wellcomeopenres.14826.1","article-title":"Open-access bacterial population genomics: BIGSdb software, the PubMLST.org website and their applications","volume":"3","author":"KA Jolley","year":"2018","journal-title":"Wellcome Open Res"},{"issue":"1","key":"pone.0346097.ref095","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1093\/molbev\/msu300","article-title":"IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies","volume":"32","author":"L-T Nguyen","year":"2015","journal-title":"Mol Biol Evol"},{"issue":"2","key":"pone.0346097.ref096","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1093\/molbev\/msx281","article-title":"UFBoot2: improving the ultrafast bootstrap approximation","volume":"35","author":"DT Hoang","year":"2018","journal-title":"Mol Biol Evol"},{"issue":"6","key":"pone.0346097.ref097","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/nmeth.4285","article-title":"ModelFinder: fast model selection for accurate phylogenetic estimates","volume":"14","author":"S Kalyaanamoorthy","year":"2017","journal-title":"Nat Methods"},{"key":"pone.0346097.ref098","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkae268","article-title":"Interactive Tree of Life (iTOL) v6: recent updates to the phylogenetic tree display and annotation tool","volume":"52","author":"I Letunic","year":"2024","journal-title":"Nucleic Acids Res"},{"key":"pone.0346097.ref099","doi-asserted-by":"crossref","first-page":"1469411","DOI":"10.3389\/fmicb.2024.1469411","article-title":"Meta-analysis of the Vmp-like sequences of Lyme disease Borrelia: evidence for the evolution of an elaborate antigenic variation system","volume":"15","author":"SJ Norris","year":"2024","journal-title":"Front Microbiol"},{"key":"pone.0346097.ref100","doi-asserted-by":"crossref","first-page":"105502","DOI":"10.1016\/j.meegid.2023.105502","article-title":"Genome diversity of Borrelia garinii in marine transmission cycles does not match host associations but reflects the strains evolutionary history","volume":"115","author":"G Margos","year":"2023","journal-title":"Infect Genet Evol"},{"key":"pone.0346097.ref101","volume-title":"R: a language and environment for statistical computing","author":"R Core Team","year":"2024"},{"key":"pone.0346097.ref102","volume-title":"Elegant graphics for data analysis","author":"H Wickham","year":"2016"},{"key":"pone.0346097.ref103","volume-title":"PropCIs: various confidence interval methods for proportions","author":"R Scherer","year":"2018"},{"issue":"1","key":"pone.0346097.ref104","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v067.i01","article-title":"Fitting linear mixed-effects models using lme4","volume":"67","author":"D Bates","year":"2015","journal-title":"J Stat Softw"},{"key":"pone.0346097.ref105","volume-title":"Arm: data analysis using regression and multilevel\/hierarchical models","author":"A Gelman","year":"2024"},{"issue":"4","key":"pone.0346097.ref106","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1111\/j.1469-185X.2010.00141.x","article-title":"Repeatability for Gaussian and non-Gaussian data: a practical guide for biologists","volume":"85","author":"S Nakagawa","year":"2010","journal-title":"Biol Rev Camb Philos Soc"},{"key":"pone.0346097.ref107","volume-title":"ggpubr: \u201cggplot2\u201d based publication ready plots","author":"A Kassambara","year":"2025"},{"key":"pone.0346097.ref108","volume-title":"rstatix: pipe-friendly framework for basic statistical tests","author":"A Kassambara","year":"2023"},{"key":"pone.0346097.ref109","doi-asserted-by":"crossref","DOI":"10.18637\/jss.v069.c02","article-title":"cooccur: probabilistic species co-occurrence analysis inR","volume":"69","author":"DM Griffith","year":"2016","journal-title":"J Stat Soft"},{"issue":"1","key":"pone.0346097.ref110","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1128\/jb.179.1.217-227.1997","article-title":"Homology throughout the multiple 32-kilobase circular plasmids present in Lyme disease spirochetes","volume":"179","author":"S Casjens","year":"1997","journal-title":"J Bacteriol"},{"issue":"2","key":"pone.0346097.ref111","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1111\/mmi.12271","article-title":"Lipoprotein succession in Borrelia burgdorferi: similar but distinct roles for OspC and VlsE at different stages of mammalian infection","volume":"89","author":"K Tilly","year":"2013","journal-title":"Mol Microbiol"},{"issue":"2","key":"pone.0346097.ref112","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0092-8674(00)80206-8","article-title":"Antigenic variation in Lyme disease borreliae by promiscuous recombination of VMP-like sequence cassettes","volume":"89","author":"JR Zhang","year":"1997","journal-title":"Cell"},{"issue":"4","key":"pone.0346097.ref113","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1111\/mec.16805","article-title":"Out of Asia? Expansion of Eurasian Lyme borreliosis causing genospecies display unique evolutionary trajectories","volume":"32","author":"RE Rollins","year":"2023","journal-title":"Mol Ecol"},{"issue":"8","key":"pone.0346097.ref114","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1038\/s44319-025-00378-9","article-title":"Borrelia burgdorferi lacking all cp32 prophage plasmids retains full infectivity in mice","volume":"26","author":"C Hillman","year":"2025","journal-title":"EMBO Rep"},{"issue":"8","key":"pone.0346097.ref115","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1128\/iai.56.8.1831-1836.1988","article-title":"Changes in infectivity and plasmid profile of the Lyme disease spirochete, Borrelia burgdorferi, as a result of in vitro cultivation","volume":"56","author":"TG Schwan","year":"1988","journal-title":"Infect Immun"},{"issue":"3","key":"pone.0346097.ref116","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1128\/jcm.26.3.475-478.1988","article-title":"Plasmid analysis of Borrelia burgdorferi, the Lyme disease agent","volume":"26","author":"AG Barbour","year":"1988","journal-title":"J Clin Microbiol"},{"issue":"6","key":"pone.0346097.ref117","doi-asserted-by":"crossref","first-page":"2206","DOI":"10.1128\/iai.63.6.2206-2212.1995","article-title":"High- and low-infectivity phenotypes of clonal populations of in vitro-cultured Borrelia burgdorferi","volume":"63","author":"SJ Norris","year":"1995","journal-title":"Infect Immun"},{"issue":"1","key":"pone.0346097.ref118","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.plasmid.2011.02.006","article-title":"Loss of plasmids of Borrelia burgdorferi sensu lato during prolonged in vitro cultivation","volume":"66","author":"UG 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spirochete","volume":"13","author":"CN Takacs","year":"2022","journal-title":"Nat Commun"},{"issue":"5","key":"pone.0346097.ref122","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1111\/j.1365-2958.1993.tb01641.x","article-title":"Linear chromosomal physical and genetic map of Borrelia burgdorferi, the Lyme disease agent","volume":"8","author":"S Casjens","year":"1993","journal-title":"Mol Microbiol"},{"issue":"16","key":"pone.0346097.ref123","doi-asserted-by":"crossref","first-page":"5251","DOI":"10.1128\/jb.174.16.5251-5257.1992","article-title":"Linear- and circular-plasmid copy numbers in Borrelia burgdorferi","volume":"174","author":"J Hinnebusch","year":"1992","journal-title":"J Bacteriol"},{"issue":"7","key":"pone.0346097.ref124","article-title":"Organization and replicon interactions within the highly segmented genome of Borrelia burgdorferi","volume":"19","author":"Z Ren","year":"2023","journal-title":"PLoS 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