{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T22:19:14Z","timestamp":1780352354437,"version":"3.54.1"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T00:00:00Z","timestamp":1775088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"The Ministry of Education, Culture, Sports, Science, and Technology of Japan","doi-asserted-by":"crossref","award":["#17H03806, #21H02117 and # 24K08715"],"award-info":[{"award-number":["#17H03806, #21H02117 and # 24K08715"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003176","name":"Ministerio de Educaci\u00f3n, Cultura y Deporte","doi-asserted-by":"publisher","award":["#18K05393"],"award-info":[{"award-number":["#18K05393"]}],"id":[{"id":"10.13039\/501100003176","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"the Ministry of Education, Culture, Sports, Science, and Technology of Japan","doi-asserted-by":"crossref","award":["#18K14377"],"award-info":[{"award-number":["#18K14377"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries, and Food Industry","award":["#957613"],"award-info":[{"award-number":["#957613"]}]},{"name":"the Mishima Kaiun Memorial Foundation"},{"name":"the Faculty of Life and Environmental Sciences at Shimane University"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Coenzyme Q (CoQ) is an essential component of the electron transport chain, and ten genes involved in CoQ biosynthesis have been identified in\n                    <jats:italic>Schizosaccharomyces pombe<\/jats:italic>\n                    . To gain further insight into CoQ biosynthesis, we screened the Bioneer gene-deletion library and found that the\n                    <jats:italic>\u0394pos5<\/jats:italic>\n                    strain produced only 0.2-fold of the wild-type CoQ\n                    <jats:sub>10<\/jats:sub>\n                    level. Pos5 shares homology with\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    Pos5 (ScPos5), a mitochondrial NADH (or NAD\n                    <jats:sup>+<\/jats:sup>\n                    ) kinase that generates NADPH (or NADP\n                    <jats:sup>+<\/jats:sup>\n                    ). Heterologous expression of\n                    <jats:italic>ScPOS5<\/jats:italic>\n                    in the\n                    <jats:italic>S. pombe<\/jats:italic>\n                    \u0394\n                    <jats:italic>pos5<\/jats:italic>\n                    strain recovered CoQ content to 0.9-fold of the wild-type level, indicating functional conservation of Pos5 between the two yeasts. Consistently, CoQ\n                    <jats:sub>6<\/jats:sub>\n                    level in\n                    <jats:italic>\u0394Scpos5<\/jats:italic>\n                    was decreased to 0.2-fold of that in the wild-type strain. The\n                    <jats:italic>\u0394pos5<\/jats:italic>\n                    strain exhibited several phenotypes characteristic of CoQ-deficient\n                    <jats:italic>S. pombe<\/jats:italic>\n                    , including inability to grow on non-fermentable carbon sources, hypersensitivity to oxidative stress, and high sulfide production. Among CoQ biosynthetic enzymes, Coq6 monooxygenase is thought to utilize NADPH. Supplementation with VA or PHB partially restored CoQ production in the\n                    <jats:italic>\u0394pos5<\/jats:italic>\n                    strain, while overexpression of\n                    <jats:italic>coq6<\/jats:italic>\n                    had negligible effect. These findings suggest that Pos5 is required for the earlier step of CoQ biosynthesis.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0346295","type":"journal-article","created":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T17:24:51Z","timestamp":1775150691000},"page":"e0346295","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mitochondrial NAD kinase Pos5 is required for CoQ biosynthesis in 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