{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T18:08:25Z","timestamp":1775239705362,"version":"3.50.1"},"reference-count":71,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Hepatocellular carcinoma (HCC) has a very significant mortality rate and is one of the most common cancers worldwide.\n                    <jats:italic>Jacaranda mimosifolia<\/jats:italic>\n                    is reported to have potential antitumor activities against various human cancers. However, the effects of\n                    <jats:italic>J. mimosifolia<\/jats:italic>\n                    on HCC are yet elusive. This study aimed to investigate the anti-HCC potential of methanolic extract of\n                    <jats:italic>J. mimosifolia<\/jats:italic>\n                    leaves using\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    studies and a network pharmacology approach. The effect of\n                    <jats:italic>J. mimosifolia<\/jats:italic>\n                    extract was assessed on Huh-7.5 cells using MTT assay, wound healing assay, and DNA fragmentation assay. These experiments found that\n                    <jats:italic>J. mimosifolia<\/jats:italic>\n                    extract significantly suppressed Huh-7.5 cell proliferation, impaired cell migration, and induced cell apoptosis. The real-time PCR validated the upregulation of\n                    <jats:italic>p53<\/jats:italic>\n                    and\n                    <jats:italic>Bax<\/jats:italic>\n                    , alongside the downregulation of\n                    <jats:italic>AFP<\/jats:italic>\n                    and\n                    <jats:italic>GPC3 in<\/jats:italic>\n                    Huh-7.5 cells after treatment with\n                    <jats:italic>J. mimosifolia<\/jats:italic>\n                    extract.\n                    <jats:italic>In vivo<\/jats:italic>\n                    experiments confirmed the hepatoprotective effects of\n                    <jats:italic>J. mimosifolia<\/jats:italic>\n                    extract in mice models with CCl\n                    <jats:sub>4<\/jats:sub>\n                    -induced hepatic injury. In addition, through network pharmacological analysis,\n                    <jats:italic>J. mimosifolia<\/jats:italic>\n                    was found to play a critical role against HCC via targeting multiple potential targets and pathways. Docking analysis identified apigenin and kaempferol with the lowest binding energy against PTGS2 and EGFR, respectively, while flavonol glycoside showed the lowest binding energy against MMP9. However, detailed research is needed to isolate the potential phytochemicals from\n                    <jats:italic>J. mimosifolia<\/jats:italic>\n                    against HCC.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0346325","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T17:38:07Z","timestamp":1775237887000},"page":"e0346325","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anti-hepatocellular carcinoma activity of Jacaranda mimosifolia through experimental validation and network pharmacology"],"prefix":"10.1371","volume":"21","author":[{"given":"Ayesha","family":"Bibi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Hamza","family":"Afandi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azra","family":"Mehmood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5495-805X","authenticated-orcid":true,"given":"Usman Ali","family":"Ashfaq","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Shareef","family":"Masoud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohsin Ahmad","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7971-5874","authenticated-orcid":true,"given":"Rashid","family":"Bhatti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,4,3]]},"reference":[{"key":"pone.0346325.ref001","doi-asserted-by":"crossref","first-page":"795548","DOI":"10.3389\/fonc.2021.795548","article-title":"Recent progress in understanding the action of natural compounds at novel therapeutic drug targets for the treatment of liver cancer","volume":"11","author":"Y Zheng","year":"2022","journal-title":"Front Oncol"},{"key":"pone.0346325.ref002","doi-asserted-by":"crossref","unstructured":"Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. 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