{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T18:00:37Z","timestamp":1780941637761,"version":"3.54.1"},"reference-count":22,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100016105","name":"Quzhou Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["2024K128"],"award-info":[{"award-number":["2024K128"]}],"id":[{"id":"10.13039\/501100016105","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"\n Background<\/jats:title>\n After the relaxation of COVID\u201119 containment measures, we investigated the pathogen spectrum and epidemiological characteristics of acute respiratory infections (ARIs) in Quzhou City from 2023 to 2024.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n This study aimed to investigate the pathogen spectrum and epidemiological characteristics of acute respiratory infections (ARIs) in Quzhou City from 2023 to 2024, providing a scientific basis for local prevention and control strategies.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n A total of 2,800 respiratory specimens were collected from November 2023 to July 2024, comprising 1,960 influenza-like illness (ILI) cases from outpatient\/emergency departments and 840 severe acute respiratory infection (SARI) cases from inpatient departments. All samples were tested for 13 common respiratory pathogens using multiplex fluorescence quantitative PCR. Etiological and epidemiological analyses were performed based on detection results and case information.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n The overall ARI positivity rate was 59.28% (1,660\/2,800), with a male-to-female ratio of 1.07:1 (1,447\/1,353). The three most prevalent pathogens were influenza virus (Flu, 23.21%, 650\/2,800), Streptococcus pneumoniae (SP, 13.14%, 368\/2,800), and adenovirus (ADV, 8.39%, 235\/2,800). Single pathogen infections accounted for 73.55% (1,221\/1,660) of positive cases, while co-infections with two or more pathogens accounted for 26.45% (439\/1,660), yielding an overall co-infection rate of 15.68% (439\/2,800). No significant gender difference was observed in detection rates. However, significant differences were found across case types, temporal periods, age groups, and geographic regions (P\u2009<\u20090.01). Children aged \u22645 years exhibited the highest positivity rate (78.00%, 378\/525), while adults aged \u226565 years showed the lowest (34.53%, 144\/417). Among surveillance regions, Kaihua County had the highest positivity rate (72.47%), and Changshan County the lowest (40.55%).<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Multiple respiratory pathogens and co-infections are prevalent in Quzhou City, with distinct age-specific and seasonal patterns. These findings underscore the need for continuous multi-pathogen surveillance and integrated prevention strategies for influenza and other respiratory infectious diseases in the post-pandemic era.<\/jats:p>\n <\/jats:sec>","DOI":"10.1371\/journal.pone.0346441","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T17:30:42Z","timestamp":1780939842000},"page":"e0346441","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pathogen spectrum and epidemiology of respiratory tract infections in Quzhou, Eastern China, from November 2023 to July 2024: A post\u2011COVID\u201119 surveillance study"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8309-7375","authenticated-orcid":true,"given":"Rui-jun","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Lyu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-ling","family":"You","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-teng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing-dong","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,6,8]]},"reference":[{"key":"pone.0346441.ref001","unstructured":"WHO. 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