{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:12:47Z","timestamp":1790140367946,"version":"4.0.1"},"reference-count":29,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Discipline of Pudong New Area Health Commission Discipline Construction Plan","award":["Project No.: PWZxk2022-10"],"award-info":[{"award-number":["Project No.: PWZxk2022-10"]}]},{"name":"Science and New Quality Specialty (Special Disease) of Clinical Medicine for Peak Plateau Discipline Construction in Pudong New Area in 2025","award":["Project No.: 2025-PWXZ-07"],"award-info":[{"award-number":["Project No.: 2025-PWXZ-07"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Understanding how to effectively treat neurological deficits after stroke remains difficult, as both the therapeutic targets and clinical outcomes are still uncertain. Although intermittent Theta burst stimulation (iTBS) has recently been shown to protect neural tissue in stroke-induced rats, its underlying biology has not been clearly defined. To clarify how iTBS acts at the cellular level, we applied single-cell transcriptome sequencing to a rat model of middle cerebral artery occlusion. After receiving the iTBS protocol, rats underwent behavioral assessments to determine the extent of neurological recovery. We then profiled gene expression across neuronal subtypes using 10x Genomics technology. iTBS markedly alleviated neurological impairment, and single-cell profiling indicated that the stimulation was associated with changes in microglial transcripts (e.g.,\n                    <jats:italic>Vav3<\/jats:italic>\n                    and\n                    <jats:italic>Cblb<\/jats:italic>\n                    ) alongside coordinated responses in other cell populations. Together, these data suggest that iTBS is associated with changes in post-ischemic microglial state composition and transcriptional programs, highlighting candidate neuroinflammatory processes for future mechanistic investigation.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0346888","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T18:00:41Z","timestamp":1776708041000},"page":"e0346888","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Single-cell RNA sequencing identifies microglial state changes associated with iTBS after ischemia\u2013reperfusion injury"],"prefix":"10.1371","volume":"21","author":[{"given":"Yingying","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yining","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6032-8425","authenticated-orcid":true,"given":"Xia","family":"Bi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,4,20]]},"reference":[{"issue":"1","key":"pone.0346888.ref001","first-page":"89","article-title":"Endovascular thrombectomy for acute large ischemic strokes","volume":"389","author":"DPO Kaiser","year":"2023","journal-title":"N Engl J 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