{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:37:20Z","timestamp":1791995840459,"version":"4.4.0"},"reference-count":31,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:00:00Z","timestamp":1776211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The 2-Minute Walk Test (2MWT) is a simple and reliable test used by clinicians to evaluate mobility gains in stroke populations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine the minimally clinically important difference (MCID) for the 2-Minute Walk Test (2MWT) in subacute stroke populations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>This was a prospective cohort study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Setting<\/jats:title>\n                    <jats:p>The study was carried out across seven rehabilitation centers in Pakistan.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec005\">\n                    <jats:title>Participants<\/jats:title>\n                    <jats:p>150 adults (&lt;180 days post-stroke),with a confirmed ischemic or hemorrhagic stroke, able to walk at least 10 meters, and Mini-Mental State Examination score \u226524.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec006\">\n                    <jats:title>Interventions<\/jats:title>\n                    <jats:p>Participants underwent 6\u20138 weeks of standard rehabilitation, including physical therapy (gait and balance training) and, in some centers, robotic gait training. Interventions varied by center but followed standardized gait and balance goals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec007\">\n                    <jats:title>Main outcome measures<\/jats:title>\n                    <jats:p>The 2MWT was the primary outcome measure whereas, the Activities-specific Balance Confidence gait subscale (ABC_Gait), Global Perceived Effect (GPE) scale, and Modified Barthel Index (MBI) were the secondary outcome measures. The MCID was estimated using an anchor-based approach (ABC_Gait \u226510% improvement), validated by distribution-based methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec008\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The 2MWT MCID was 33 meters (95% CI: 30\u201336 meters), with 87% sensitivity and 82% specificity. A strong correlation was observed between 2MWT and ABC_Gait change scores (r = 0.68, p &lt; 0.001). The mean 2MWT distance improved from 62.5\u2009\u00b1\u200938.4 meters to 98.7\u2009\u00b1\u200942.1 meters (p &lt; 0.001). Subgroup analyses showed that MCID was consistent across age groups, stroke types, and intervention modalities.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec009\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The 33 meters MCID for the 2MWT is a reliable and patient-centered benchmark for assessing mobility gains in subacute stroke populations. Validation in chronic stroke populations and exploration of emerging interventions like virtual reality (VR) is needed to extend the 2MWT MCID\u2019s applicability.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0347056","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T17:59:55Z","timestamp":1776275995000},"page":"e0347056","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Validating the 2-minute walk test MCID for subacute stroke patients: A Pakistani multicenter cohort analysis"],"prefix":"10.1371","volume":"21","author":[{"given":"Muslim","family":"Khan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hafiz Shehzad","family":"Muzamil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0124-4739","authenticated-orcid":true,"given":"Ahmad M","family":"Osailan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayman Abdullah","family":"Alhammad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samiullah","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mazhar","family":"Mushtaq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6946-8435","authenticated-orcid":true,"given":"Engy BadrEldin Saleh","family":"Moustafa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theeb Naif S.","family":"Alsalem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8343-4479","authenticated-orcid":true,"given":"Edward","family":"Muteesasira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,4,15]]},"reference":[{"issue":"5","key":"pone.0347056.ref001","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1080\/10749357.2024.2417643","article-title":"The minimally clinically important difference in the 2-minute walk test for people in the subacute phase after a stroke","volume":"32","author":"T Bowman","year":"2025","journal-title":"Top Stroke Rehabil"},{"issue":"4","key":"pone.0347056.ref002","doi-asserted-by":"crossref","first-page":"773","DOI":"10.47391\/JPMA.7152","article-title":"An eight-week single-blind randomised controlled trial on dual task balance training and motor imagery practice in ischaemic stroke survivors","volume":"74","author":"M Zafar","year":"2024","journal-title":"J Pak Med Assoc"},{"issue":"1","key":"pone.0347056.ref003","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1097\/PHM.0000000000001272","article-title":"Mobility Disorders in Stroke, Parkinson Disease, and Multiple Sclerosis: A Multicenter Cross-Sectional Study","volume":"99","author":"D Cattaneo","year":"2020","journal-title":"Am J Phys Med Rehabil"},{"issue":"1","key":"pone.0347056.ref004","doi-asserted-by":"crossref","first-page":"15059","DOI":"10.1038\/s41598-025-98227-1","article-title":"Evaluation of balance and orthotic gait training techniques for rehabilitation in hemiplegic stroke patients","volume":"15","author":"S Tariq","year":"2025","journal-title":"Sci Rep"},{"key":"pone.0347056.ref005","first-page":"351","volume-title":"Rehabilitation in subacute and chronic stage after stroke. 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