{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T18:13:12Z","timestamp":1778695992354,"version":"3.51.4"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u00e2\u20ac\u0153Xinglin Scholar\u00e2\u20ac hospital special program for discipline talent research enhancement","award":["XJ2023009601"],"award-info":[{"award-number":["XJ2023009601"]}]},{"name":"Sichuan medical association orthopedics (Shang Antong) special research project","award":["2024SAT01"],"award-info":[{"award-number":["2024SAT01"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background and Objective<\/jats:title>\n                    <jats:p>KIF11, a mitotic kinesin, has been implicated in oncogenesis, but its specific role and mechanism in RA pathogenesis remain largely unexplored. We aim to explore functional role and molecular mechanism of KIF11 to promote RA progression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Bioinformatics analysis was first performed on public RA datasets (GSE55457, GSE55235, GSE2053, GSE12021, and GSE1919) to identify KIF11-associated DEGs and their enriched pathways using GO\/KEGG and GSEA analysis. Lentivirus-mediated shRNA was employed to knock down KIF11 expression in MH7A cells and M1-polarized macrophages. The effects on macrophage activation were assessed by FACS for the surface marker CD86. The expression of inflammatory cytokines were measured by quantitative PCR and ELISA, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Bioinformatic analysis showd that KIF11-associated genes were significantly enriched in immune activation pathways, especially adaptive immune system and cytokine-cytokine receptor interaction. In vitro data demonstrated that KIF11 knockdown suppressed the M1 macrophage phenotype, with a marked decreased expression of CD86. Furthermore, KIF11 deficiency led to a profound decrease in the mRNA levels and protein secretion of cytokines in M1 macrophages.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Depletion of KIF11 markedly inhibited the proliferation, migration, and inflammatory cytokine secretion in MH7A and attenuated the maturation of M1 macrophages, as well as the production of inflammatory cytokines, suggesting its pivotal role in RA pathology.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0347313","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T17:26:31Z","timestamp":1778693191000},"page":"e0347313","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["KIF11 promotes rheumatoid arthritis pathogenesis by activating M1 macrophage polarization and promoting inflammatory cytokine secretion"],"prefix":"10.1371","volume":"21","author":[{"given":"Zhaonan","family":"Ban","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongjie","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hang","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengjiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhangzhen","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanquan","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3375-8088","authenticated-orcid":true,"given":"Shuxing","family":"Xing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,5,13]]},"reference":[{"issue":"9","key":"pone.0347313.ref001","article-title":"Identification 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