{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:53:30Z","timestamp":1791309210300,"version":"4.3.1"},"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    The cockroach\n                    <jats:italic>Blattella germanica<\/jats:italic>\n                    possesses panoistic ovaries, in which oocytes lack nurse cells and therefore need to rely on their own transcriptional activity to support embryogenesis. Ovarian development in this species involves the development of a single basal ovarian follicle (BOF) per gonadotropic cycle, a process strictly regulated by endocrine signals, primarily juvenile hormone and ecdysone, which act at both the transcriptional and translational levels. In addition, transcriptional activity in these ovaries is necessary for both regulating and genome protection, and at this level, PIWI-interacting RNAs (piRNAs) play an essential role. Although insect ovaries are known to be particularly rich in piRNAs, their function in ovary maturation is still not well defined. For this purpose, we characterize the piRNA expression dynamics in the\n                    <jats:italic>B. germanica<\/jats:italic>\n                    ovary, which shows coordinated fluctuations. The piRNA expression remains stable in previtellogenic ovaries, whereas vitellogenic ovaries show pronounced changes. Moreover, vitellogenic ovaries exhibit reduced piRNA diversity due to strong enrichment of a subset of highly expressed piRNAs. Our data show that although piRNAs predominantly map to transposable elements, particularly LINEs, there is a notable increase in mRNA-derived piRNAs toward the end of the cycle. Our results indicate that piRNAs may play regulatory roles in modulating both TEs and mRNAs during BOF maturation, likely linked to changes in the follicular cell program.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0347365","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:24:59Z","timestamp":1791307499000},"page":"e0347365","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["The dynamics of piRNA expression in Blattella germanica ovaries"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1592-740X","authenticated-orcid":true,"given":"David","family":"Pujal","sequence":"first","affiliation":[{"name":"Institute of Evolutionary Biology (CSIC- Universitat Pompeu Fabra)","place":["Barcelona, Spain"]},{"name":"University of Vic - Central University of Catalonia","place":["Vic, Barcelona, Spain"],"department":["Department of Biosciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4540-0131","authenticated-orcid":true,"given":"Guillem","family":"Ylla","sequence":"additional","affiliation":[{"name":"Biophysics and Biotechnology, Jagiellonian University","place":["Krak\u00f3w, Poland"],"department":["Faculty of Biochemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agustina","family":"Pascual","sequence":"additional","affiliation":[{"name":"Centro de BioInvestigaciones (CeBio-CICBA), Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA)","place":["Pergamino, Argentina"]},{"name":"Centro de Investigaciones y Transferencias del Noroeste de la Provincia de Buenos Aires (CITNOBA-CONICET)","place":["Pergamino, Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9231-2356","authenticated-orcid":true,"given":"Josep","family":"Bau","sequence":"additional","affiliation":[{"name":"University of Vic - Central University of Catalonia","place":["Vic, Barcelona, Spain"],"department":["Department of Biosciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4500-2718","authenticated-orcid":true,"given":"Maria-Dolors","family":"Piulachs","sequence":"additional","affiliation":[{"name":"Institute of Evolutionary Biology (CSIC- Universitat Pompeu Fabra)","place":["Barcelona, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"issue":"2","key":"pone.0347365.ref001","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.bbagrm.2014.05.025","article-title":"Ecdysone signalling and ovarian development in insects: from stem cells to ovarian follicle formation","volume":"1849","author":"X Belles","year":"2015","journal-title":"Biochim Biophys Acta"},{"key":"pone.0347365.ref002","article-title":"The German cockroach as a model in insect development and reproduction in an endocrine context","volume":"66","author":"X Belles","year":"2024","journal-title":"Adv In Insect Phys"},{"key":"pone.0347365.ref003","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-032-06766-1_8","article-title":"Oogenesis of the German cockroach: from morphology to regulation.","volume-title":"Evo-Devo: In Search of New Model Species","author":"MD. 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Genet"},{"key":"pone.0347365.ref010","doi-asserted-by":"crossref","first-page":"104891","DOI":"10.1016\/j.jinsphys.2025.104891","article-title":"A piRNA modulates the levels of 20-hydroxyecdysone in the ovary of the German cockroach","volume":"166","author":"J Gonzalvo","year":"2025","journal-title":"J Insect Physiol"},{"issue":"9","key":"pone.0347365.ref011","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1010912","article-title":"Non-gonadal somatic piRNA pathways ensure sexual differentiation, larval growth, and wing development in silkworms","volume":"19","author":"T Kiuchi","year":"2023","journal-title":"PLoS Genet"},{"issue":"17","key":"pone.0347365.ref012","doi-asserted-by":"crossref","DOI":"10.1242\/dev.161786","article-title":"piRNAs and PIWI proteins: regulators of gene expression in development and stem cells","volume":"145","author":"P Rojas-R\u00edos","year":"2018","journal-title":"Development"},{"issue":"2","key":"pone.0347365.ref013","doi-asserted-by":"crossref","first-page":"187","DOI":"10.3390\/insects14020187","article-title":"What are the functional roles of Piwi proteins and piRNAs in insects?","volume":"14","author":"D Santos","year":"2023","journal-title":"Insects"},{"issue":"5","key":"pone.0347365.ref014","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1002\/jez.b.22815","article-title":"Diversity of piRNA expression patterns during the ontogeny of the German cockroach","volume":"330","author":"N Llonga","year":"2018","journal-title":"J Exp Zool Part B Mol Dev Evol"},{"key":"pone.0347365.ref015","first-page":"93","article-title":"The ovary is a source of circulating ecdysteroids in Blattella germanica","volume":"92","author":"I Roma\u00f1a","year":"1995","journal-title":"Eur J Entomol"},{"issue":"11","key":"pone.0347365.ref016","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0113850","article-title":"Unlike in Drosophila Meroistic Ovaries, hippo represses notch in Blattella germanica Panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells","volume":"9","author":"P Irles","year":"2014","journal-title":"PLoS One"},{"key":"pone.0347365.ref017","doi-asserted-by":"crossref","first-page":"103407","DOI":"10.1016\/j.ibmb.2020.103407","article-title":"Eyes absent in the cockroach panoistic ovaries regulates proliferation and differentiation through ecdysone signalling","volume":"123","author":"S Ramos","year":"2020","journal-title":"Insect Biochem Mol Biol"},{"key":"pone.0347365.ref018","doi-asserted-by":"crossref","first-page":"103935","DOI":"10.1016\/j.ibmb.2023.103935","article-title":"Diverse functions of the ecdysone receptor (EcR) in the panoistic ovary of the German cockroach","volume":"156","author":"M Rumbo","year":"2023","journal-title":"Insect Biochem Mol Biol"},{"issue":"8","key":"pone.0347365.ref019","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/boc.201500003","article-title":"Crosstalk of EGFR signalling with Notch and Hippo pathways to regulate cell specification, migration and proliferation in cockroach panoistic ovaries","volume":"107","author":"N Elshaer","year":"2015","journal-title":"Biol Cell"},{"issue":"1","key":"pone.0347365.ref020","doi-asserted-by":"crossref","DOI":"10.1111\/j.1365-3032.1995.tb00801.x","article-title":"Patterns of haemolymph vitellogenin and ovarian vitellin in the German cockroach, and the role of Juvenile Hormone","volume":"20","author":"D Martin","year":"1995","journal-title":"Physiol Entomol"},{"key":"pone.0347365.ref021","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1186\/1471-2164-10-206","article-title":"Identifying genes related to choriogenesis in insect panoistic ovaries by Suppression Subtractive Hybridization","volume":"10","author":"P Irles","year":"2009","journal-title":"BMC Genomics"},{"key":"pone.0347365.ref022","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1673\/031.006.4301","article-title":"Juvenile hormone titer versus juvenile hormone synthesis in female nymphs and adults of the German cockroach, Blattella germanica","volume":"6","author":"K Treiblmayr","year":"2006","journal-title":"J Insect Sci"},{"issue":"8","key":"pone.0347365.ref023","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1111\/boc.201100044","article-title":"Dicer-1 is a key enzyme in the regulation of oogenesis in panoistic ovaries","volume":"104","author":"ED Tanaka","year":"2012","journal-title":"Biol Cell"},{"issue":"4","key":"pone.0347365.ref024","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"issue":"1","key":"pone.0347365.ref025","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1093\/nar\/30.1.207","article-title":"Gene Expression Omnibus: NCBI gene expression and hybridization array data repository","volume":"30","author":"R Edgar","year":"2002","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"pone.0347365.ref026","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1038\/s41559-017-0459-1","article-title":"Hemimetabolous genomes reveal molecular basis of termite eusociality","volume":"2","author":"MC Harrison","year":"2018","journal-title":"Nat Ecol Evol"},{"issue":"6","key":"pone.0347365.ref027","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","article-title":"BEDTools: a flexible suite of utilities for comparing genomic features","volume":"26","author":"AR Quinlan","year":"2010","journal-title":"Bioinformatics"},{"key":"pone.0347365.ref028","author":"S Andrews","year":"2010"},{"issue":"15","key":"pone.0347365.ref029","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"AM Bolger","year":"2014","journal-title":"Bioinformatics"},{"issue":"1","key":"pone.0347365.ref030","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads","volume":"17","author":"M Martin","year":"2011","journal-title":"EMBnet.journal"},{"issue":"4","key":"pone.0347365.ref031","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"B Langmead","year":"2012","journal-title":"Nat Methods"},{"issue":"2","key":"pone.0347365.ref032","doi-asserted-by":"crossref","DOI":"10.1093\/gigascience\/giab008","article-title":"Twelve years of SAMtools and BCFtools","volume":"10","author":"P Danecek","year":"2021","journal-title":"Gigascience"},{"issue":"8","key":"pone.0347365.ref033","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1003118","article-title":"Software for computing and annotating genomic ranges","volume":"9","author":"M Lawrence","year":"2013","journal-title":"PLoS Comput Biol"},{"issue":"22","key":"pone.0347365.ref034","doi-asserted-by":"crossref","first-page":"3645","DOI":"10.1093\/bioinformatics\/btx469","article-title":"ggseqlogo: a versatile R package for drawing sequence logos","volume":"33","author":"O Wagih","year":"2017","journal-title":"Bioinformatics"},{"key":"pone.0347365.ref035","first-page":"33","article-title":"ggplot2: Elegant Graphics for Data Analysis.","volume-title":"Toolbox","author":"H. Wickham","year":"2016"},{"issue":"1","key":"pone.0347365.ref036","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1186\/s12864-017-4177-5","article-title":"Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germ-band types","volume":"18","author":"G Ylla","year":"2017","journal-title":"BMC Genomics"},{"issue":"12","key":"pone.0347365.ref037","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"MI Love","year":"2014","journal-title":"Genome Biol"},{"issue":"8","key":"pone.0347365.ref038","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkz114","article-title":"The R package Rsubread is easier, faster, cheaper and better for alignment and quantification of RNA sequencing reads","volume":"47","author":"Y Liao","year":"2019","journal-title":"Nucleic Acids Res"},{"issue":"12","key":"pone.0347365.ref039","doi-asserted-by":"crossref","first-page":"5825","DOI":"10.1093\/molbev\/msab293","article-title":"eggNOG-mapper v2: Functional Annotation, Orthology Assignments, and Domain Prediction at the Metagenomic Scale","volume":"38","author":"CP Cantalapiedra","year":"2021","journal-title":"Mol Biol Evol"},{"issue":"13","key":"pone.0347365.ref040","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1093\/bioinformatics\/btl140","article-title":"Improved scoring of functional groups from gene expression data by decorrelating GO graph structure","volume":"22","author":"A Alexa","year":"2006","journal-title":"Bioinformatics"},{"issue":"11","key":"pone.0347365.ref041","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0113850","article-title":"Unlike in Drosophila Meroistic Ovaries, hippo represses notch in Blattella germanica Panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells","volume":"9","author":"P Irles","year":"2014","journal-title":"PLoS One"},{"issue":"6","key":"pone.0347365.ref042","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.3390\/cells9061491","article-title":"Conserved small nucleotidic elements at the origin of concerted piRNA biogenesis from genes and lncRNAs","volume":"9","author":"S Jensen","year":"2020","journal-title":"Cells"},{"issue":"5","key":"pone.0347365.ref043","doi-asserted-by":"crossref","DOI":"10.1016\/j.molcel.2018.08.007","article-title":"A Single Mechanism of Biogenesis, Initiated and Directed by PIWI Proteins, Explains piRNA Production in Most Animals","volume":"71","author":"I Gainetdinov","year":"2018","journal-title":"Mol Cell"},{"issue":"1","key":"pone.0347365.ref044","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1038\/s41559-017-0403-4","article-title":"Pan-arthropod analysis reveals somatic piRNAs as an ancestral defence against transposable elements","volume":"2","author":"SH Lewis","year":"2018","journal-title":"Nat Ecol Evol"},{"issue":"1","key":"pone.0347365.ref045","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/1873-3468.15023","article-title":"Non\u2010gonadal expression of piRNAs is widespread across Arthropoda","volume":"599","author":"T Yamashita","year":"2024","journal-title":"FEBS Lett"},{"issue":"22","key":"pone.0347365.ref046","doi-asserted-by":"crossref","first-page":"6027","DOI":"10.1111\/mec.17162","article-title":"Age- and caste-independent piRNAs in the germline and miRNA profiles linked to caste and fecundity in the ant Temnothorax rugatulus","volume":"32","author":"A-S Seistrup","year":"2023","journal-title":"Mol Ecol"},{"issue":"5","key":"pone.0347365.ref047","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1093\/gbe\/evx087","article-title":"Contrasting Sex-and Caste-Dependent piRNA Profiles in the Transposon Depleted Haplodiploid Honeybee Apis mellifera","volume":"9","author":"W Wang","year":"2017","journal-title":"Genome Biol Evol"},{"issue":"1","key":"pone.0347365.ref048","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s12862-018-1324-9","article-title":"Diversity and evolution of the transposable element repertoire in arthropods with particular reference to insects","volume":"19","author":"M Petersen","year":"2019","journal-title":"BMC Evol Biol"},{"issue":"1","key":"pone.0347365.ref049","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1038\/s42003-021-02197-9","article-title":"Insights into the genomic evolution of insects from cricket genomes","volume":"4","author":"G Ylla","year":"2021","journal-title":"Commun Biol"},{"issue":"1","key":"pone.0347365.ref050","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1038\/s41597-025-05375-0","article-title":"Genome assembly and annotation of the parasitoid jewel wasp Nasonia oneida","volume":"12","author":"A Rana","year":"2025","journal-title":"Sci Data"},{"key":"pone.0347365.ref051","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/1471-2164-15-86","article-title":"Finding the missing honey bee genes: lessons learned from a genome upgrade","volume":"15","author":"CG Elsik","year":"2014","journal-title":"BMC Genomics"},{"issue":"3","key":"pone.0347365.ref052","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/2159256X.2017.1318201","article-title":"Transposable elements in Drosophila","volume":"7","author":"TJ McCullers","year":"2017","journal-title":"Mob Genet Elements"},{"issue":"5832","key":"pone.0347365.ref053","doi-asserted-by":"crossref","first-page":"1718","DOI":"10.1126\/science.1138878","article-title":"Genome sequence of Aedes aegypti, a major arbovirus vector","volume":"316","author":"V Nene","year":"2007","journal-title":"Science"},{"issue":"5","key":"pone.0347365.ref054","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1093\/molbev\/msq337","article-title":"Population genomics of transposable elements in Drosophila melanogaster","volume":"28","author":"DA Petrov","year":"2011","journal-title":"Mol Biol Evol"},{"issue":"1","key":"pone.0347365.ref055","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1186\/s13059-017-1304-1","article-title":"Abundant expression of somatic transposon-derived piRNAs throughout Tribolium castaneum embryogenesis","volume":"18","author":"M Ninova","year":"2017","journal-title":"Genome Biol"},{"key":"pone.0347365.ref056","doi-asserted-by":"crossref","first-page":"1481881","DOI":"10.3389\/fcell.2024.1481881","article-title":"Embryonic piRNAs target horizontally transferred vertebrate transposons in assassin bugs","volume":"12","author":"TF de Brito","year":"2024","journal-title":"Front Cell Dev Biol"},{"issue":"1","key":"pone.0347365.ref057","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1186\/s13071-016-1815-8","article-title":"Developmental piRNA profiles of the invasive vector mosquito Aedes albopictus","volume":"9","author":"P Liu","year":"2016","journal-title":"Parasit Vectors"},{"issue":"1","key":"pone.0347365.ref058","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1186\/s12859-026-06380-9","article-title":"piRAT: piRNA Annotation Tool for annotating, analyzing, and visualizing piRNAs","volume":"27","author":"D Robak","year":"2026","journal-title":"BMC Bioinform"},{"issue":"6","key":"pone.0347365.ref059","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.3390\/molecules23061302","article-title":"A Half-Century History of Applications of Antisense Oligonucleotides in Medicine, Agriculture and Forestry: We Should Continue the Journey","volume":"23","author":"VV Oberemok","year":"2018","journal-title":"Molecules"},{"key":"pone.0347365.ref060","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-0716-2380-0_1","article-title":"Functional Analysis of Individual piRNAs in Aedes aegypti Cells and Embryos Using Antisense 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