{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T18:35:03Z","timestamp":1777055703506,"version":"3.51.4"},"reference-count":27,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016341","name":"Korea University Guro Hospital","doi-asserted-by":"publisher","award":["K2512591"],"award-info":[{"award-number":["K2512591"]}],"id":[{"id":"10.13039\/100016341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["HR20C0021"],"award-info":[{"award-number":["HR20C0021"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"\n Background<\/jats:title>\n \n Candida auris<\/jats:italic>\n is an emerging multidrug-resistant yeast associated with invasive infections, healthcare-associated outbreaks, and high mortality, and is often misidentified by conventional diagnostic methods. Rapid, accurate, and scalable screening tools are essential for effective infection control, particularly in high-risk settings.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and methods<\/jats:title>\n \n We developed a multiplex loop-mediated isothermal amplification (LAMP) assay that combines a broad-range\n Candida<\/jats:italic>\n Pan target with a\n C. auris<\/jats:italic>\n \u2013specific target in a single isothermal reaction. Assay conditions were optimized for primer ratio and temperature, and analytical sensitivity was evaluated using serial dilutions of culture-derived\n C. albicans<\/jats:italic>\n and\n C. auris<\/jats:italic>\n DNA, as well as contrived specimens consisting of urine, swab, and whole-blood matrices. Clinical performance was assessed using 35\n Candida<\/jats:italic>\n -positive clinical specimens (blood, urine, ear swabs) and 94 non-infectious controls. Results were compared with\n Candida<\/jats:italic>\n Pan qPCR and\n C. auris<\/jats:italic>\n qPCR. Cross-reactivity was tested against common bacterial isolates.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Under optimized conditions (1:1 primer ratio, 64 \u00b0C), the assay allowed species-level discrimination, with\n C. auris<\/jats:italic>\n positive for both Pan and\n auris<\/jats:italic>\n channels and\n C. albicans<\/jats:italic>\n positive only for the Pan channel. The\n C. auris<\/jats:italic>\n -specific LAMP probe detected approximately 10\u00b2\u201310\u00b3 cells\/mL in culture-derived and contrived specimens, showing a 1\u20132 log improvement over\n C. auris<\/jats:italic>\n qPCR (10\u2074\u201310\u2075 cells\/mL), while the Pan LAMP channel detected\n C. auris<\/jats:italic>\n at around 10\u2075 cells\/mL. In clinical specimens, Pan LAMP detected\n Candida<\/jats:italic>\n spp. in 34\/35 cases (97.14%) versus 32\/35 (91.14%) for Pan qPCR. All\n C. auris<\/jats:italic>\n \u2013positive specimens (9\/9) were detected by the multiplex LAMP assay, compared with 6\/9 (66.7%) by Pan qPCR. All 94 non-infectious controls and all bacterial isolates tested negative, indicating 100% clinical specificity and absence of cross-reactivity.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n The multiplex\n Candida<\/jats:italic>\n Pan\/\n auris<\/jats:italic>\n LAMP assay provides a rapid, highly sensitive, and specific alternative to qPCR for\n C. auris<\/jats:italic>\n screening, while preserving broad\n Candida<\/jats:italic>\n detection in a single isothermal reaction. Its improved analytical and clinical sensitivity suggests strong potential for use in active surveillance and infection-control programs, particularly in settings where timely identification and containment of\n C. auris<\/jats:italic>\n are critical.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1371\/journal.pone.0348003","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T17:37:26Z","timestamp":1777052246000},"page":"e0348003","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a Loop-Mediated Isothermal Amplification (LAMP) for the screening of Candida auris"],"prefix":"10.1371","volume":"21","author":[{"given":"Woong Sik","family":"Jang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young 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hospital","volume":"53","author":"K Satoh","year":"2009","journal-title":"Microbiol Immunol"},{"issue":"22","key":"pone.0348003.ref002","doi-asserted-by":"crossref","first-page":"6675","DOI":"10.3390\/jcm13226675","article-title":"Candida auris: Epidemiology Update and a Review of Strategies to Prevent Spread","volume":"13","author":"JF Hayes","year":"2024","journal-title":"J Clin Med"},{"issue":"3","key":"pone.0348003.ref003","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1007\/s12281-021-00420-y","article-title":"Candida auris: Diagnostic Challenges and Emerging Opportunities for the Clinical Microbiology Laboratory","volume":"15","author":"C Keighley","year":"2021","journal-title":"Curr Fungal Infect Rep"},{"issue":"9","key":"pone.0348003.ref004","doi-asserted-by":"crossref","first-page":"913","DOI":"10.3390\/jof9090913","article-title":"An Overview on Candida auris in Healthcare Settings","volume":"9","author":"ML Cristina","year":"2023","journal-title":"J Fungi 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