{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T02:10:42Z","timestamp":1777774242084,"version":"3.51.4"},"reference-count":71,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Heat acclimation (HA) has emerged as a proven protective intervention to augment thermotolerance and mitigate heat stress (HS)-induced myocardial injury. Despite its clinical significance, the regulatory roles of noncoding RNAs associated with HA-mediated cardioprotection remain largely unexplored. This study conducted comprehensive transcriptomic profiling to delineate the molecular mechanisms underlying HA-induced cardioprotection against HS. Using well-characterized rat models of HS and HA preconditioning, we performed high-throughput sequencing on myocardial tissues to map circRNA\/lncRNA expression landscapes. Bioinformatic analyses were integrated with functional validation to identify key regulatory elements. HA preconditioning markedly attenuated HS-induced injury, reducing inflammatory cytokines (IL-1\u03b2\u2009\u2193\u200924.36%, IL-4\u2009\u2193\u200911.3%) while elevating IL-6 (+25.67%), suggesting immunomodulatory rewiring. HS leads to elevated serum HSP70 levels. Analysis identified nine core molecular candidates\u2014miR-196c-5p, miR-212-3p, miR-212-5p,\n                    <jats:italic>Rffl, Rassf1, Ppp3cc, Zbtb20, Cdh5,<\/jats:italic>\n                    and\n                    <jats:italic>Cxcl2<\/jats:italic>\n                    \u2014and delineated two central ceRNA regulatory axes: MSTRG.6276.3\u2013miR-196c-5p\u2013\n                    <jats:italic>Rffl<\/jats:italic>\n                    and MSTRG.4016.1\u2013miR-122-5p\u2013\n                    <jats:italic>Rassf<\/jats:italic>\n                    1. These networks potentially coordinate mitochondrial integrity, hypoxic adaptation, and apoptotic regulation through modulation of inflammatory signaling, metabolic homeostasis, and calcium pathways. The findings establish a noncoding RNA-defined regulatory framework for HA-mediated cardioprotection, revealing novel therapeutic targets for cardiovascular disorders triggered by thermal stress.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0348180","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T17:40:06Z","timestamp":1777484406000},"page":"e0348180","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Whole transcriptome sequencing and ceRNA regulation network profiling of heat acclimation in protecting against heat stress injury in rat myocardium"],"prefix":"10.1371","volume":"21","author":[{"given":"LiJun","family":"Fan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JiaJun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"QingHan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MingXiao","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ZiYi","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"XueWei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JunYu","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3716-1233","authenticated-orcid":true,"given":"Jing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"issue":"1","key":"pone.0348180.ref001","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1002\/j.2040-4603.2015.tb00604.x","article-title":"Human 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Liu","year":"2025","journal-title":"Eur Heart J"},{"issue":"5","key":"pone.0348180.ref008","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1519\/JSC.0000000000003970","article-title":"A 5-day heat acclimation program improves heat stress indicators while maintaining exercise capacity","volume":"35","author":"FC Wardenaar","year":"2021","journal-title":"Journal of Strength and Conditioning Research"},{"issue":"1","key":"pone.0348180.ref009","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-77358-7","article-title":"Four-month operational heat acclimatization positively affects the level of heat tolerance 6 months later[J","volume":"10","author":"A Malgoyre","year":"2020","journal-title":"Scientific reports"},{"issue":"10","key":"pone.0348180.ref010","doi-asserted-by":"crossref","first-page":"2119","DOI":"10.1007\/s00421-014-2935-5","article-title":"Changes in heart rate variability during the induction and decay of heat acclimation","volume":"114","author":"AD 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Wang","year":"2023","journal-title":"Toxicol In Vitro"},{"issue":"2","key":"pone.0348180.ref030","doi-asserted-by":"crossref","first-page":"390","DOI":"10.3390\/biom13020390","article-title":"Preliminary Transcriptome Analysis of Long Noncoding RNA in Hypothalamic-Pituitary-Mammary Gland Axis of Dairy Cows under Heat Stress","volume":"13","author":"H Zeng","year":"2023","journal-title":"Biomolecules"},{"issue":"1","key":"pone.0348180.ref031","doi-asserted-by":"crossref","first-page":"888","DOI":"10.3390\/ijms24010888","article-title":"Comprehensive Profiling of ceRNA (circRNA-miRNA-mRNA) Networks in Hypothalamic-Pituitary-Mammary Gland Axis of Dairy Cows under Heat Stress","volume":"24","author":"H Zeng","year":"2023","journal-title":"Int J Mol Sci"},{"issue":"1","key":"pone.0348180.ref032","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1186\/s12864-019-6377-7","article-title":"Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows","volume":"20","author":"H Zhang","year":"2019","journal-title":"BMC Genomics"},{"issue":"2","key":"pone.0348180.ref033","doi-asserted-by":"crossref","first-page":"270","DOI":"10.3390\/ijerph16020270","article-title":"The Effects of High-Temperature Weather on Human Sleep Quality and Appetite","volume":"16","author":"G Zheng","year":"2019","journal-title":"Int J Environ Res Public Health"},{"issue":"9","key":"pone.0348180.ref034","doi-asserted-by":"crossref","first-page":"8537","DOI":"10.3168\/jds.2018-16060","article-title":"The relationship between the number of consecutive days with heat stress and milk production of Holstein dairy cows raised in a humid continental climate","volume":"102","author":"V Ouellet","year":"2019","journal-title":"J Dairy Sci"},{"key":"pone.0348180.ref035","doi-asserted-by":"crossref","first-page":"102452","DOI":"10.1016\/j.jtherbio.2019.102452","article-title":"HSP70 plays a role in the defense of acute and chronic heat stress in Mongolian gerbils (Meriones unguiculatus)","volume":"86","author":"S-L Lou","year":"2019","journal-title":"J Therm Biol"},{"issue":"1","key":"pone.0348180.ref036","doi-asserted-by":"crossref","first-page":"175","DOI":"10.7150\/ijbs.103122","article-title":"Heat acclimation mediates cellular protection via HSP70 stabilization of HIF-1\u03b1 protein in extreme environments","volume":"21","author":"C Li","year":"2025","journal-title":"Int J Biol Sci"},{"issue":"12","key":"pone.0348180.ref037","doi-asserted-by":"crossref","first-page":"564","DOI":"10.3390\/md22120564","article-title":"Butyrolactone-I from Marine Fungal Metabolites Mitigates Heat-Stress-Induced Apoptosis in IPEC-J2 Cells and Mice Through the ROS\/PERK\/CHOP Signaling Pathway","volume":"22","author":"X Niu","year":"2024","journal-title":"Mar Drugs"},{"key":"pone.0348180.ref038","doi-asserted-by":"crossref","first-page":"177711","DOI":"10.1016\/j.ejphar.2025.177711","article-title":"OpiCa1 reduces inflammation and ROS levels counteracting heat stress-induced cardiac injury","volume":"999","author":"Z Yang","year":"2025","journal-title":"Eur J Pharmacol"},{"issue":"10","key":"pone.0348180.ref039","doi-asserted-by":"crossref","DOI":"10.1002\/iid3.1034","article-title":"Heat stress combined with lipopolysaccharide induces pulmonary microvascular endothelial cell glycocalyx inflammatory damage in vitro","volume":"11","author":"J Chen","year":"2023","journal-title":"Immun Inflamm Dis"},{"issue":"1","key":"pone.0348180.ref040","doi-asserted-by":"crossref","first-page":"4006","DOI":"10.1038\/s41598-023-30662-4","article-title":"The systemic deletion of interleukin-1\u03b1 reduces myocardial inflammation and attenuates ventricular remodeling in murine myocardial infarction","volume":"13","author":"J Lugrin","year":"2023","journal-title":"Sci Rep"},{"key":"pone.0348180.ref041","doi-asserted-by":"crossref","first-page":"103443","DOI":"10.1016\/j.redox.2024.103443","article-title":"Adipocyte-derived small extracellular vesicles exacerbate diabetic ischemic heart injury by promoting oxidative stress and mitochondrial-mediated cardiomyocyte apoptosis","volume":"79","author":"L Gan","year":"2025","journal-title":"Redox Biol"},{"issue":"1","key":"pone.0348180.ref042","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1080\/02656736.2018.1511838","article-title":"Differences in serum IL-6 response after 1\u2009\u00b0C rise in core body temperature in individuals with spinal cord injury and cervical spinal cord injury during local heat stress","volume":"35","author":"T Hashizaki","year":"2018","journal-title":"Int J Hyperthermia"},{"key":"pone.0348180.ref043","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jtherbio.2017.02.002","article-title":"Expression analysis of Toll like receptors and interleukins in Tharparkar cattle during acclimation to heat stress exposure","volume":"65","author":"J Bharati","year":"2017","journal-title":"J Therm 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Sci"},{"issue":"1","key":"pone.0348180.ref047","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.yjmcc.2011.07.010","article-title":"A phenotypic screen to identify hypertrophy-modulating microRNAs in primary cardiomyocytes","volume":"52","author":"C Jentzsch","year":"2012","journal-title":"J Mol Cell Cardiol"},{"key":"pone.0348180.ref048","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1038\/s41420-017-0011-8","article-title":"NR4A2 protects cardiomyocytes against myocardial infarction injury by promoting autophagy","volume":"4","author":"H Liu","year":"2018","journal-title":"Cell Death Discov"},{"issue":"2","key":"pone.0348180.ref049","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.yexcr.2016.12.008","article-title":"MiR-212-3p inhibits LPS-induced inflammatory response through targeting HMGB1 in murine macrophages","volume":"350","author":"W Chen","year":"2017","journal-title":"Exp Cell 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(Wars)"},{"issue":"7","key":"pone.0348180.ref053","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1080\/15376516.2021.1919948","article-title":"Neuroprotective effect of miR-212-5p on isoflurane-induced cognitive dysfunction by inhibiting neuroinflammation","volume":"31","author":"J Si","year":"2021","journal-title":"Toxicol Mech Methods"},{"key":"pone.0348180.ref054","doi-asserted-by":"crossref","first-page":"113079","DOI":"10.1016\/j.fct.2022.113079","article-title":"Maternal genetic effect on apoptosis of ovarian granulosa cells induced by cadmium","volume":"165","author":"Y Sun","year":"2022","journal-title":"Food Chem Toxicol"},{"issue":"1","key":"pone.0348180.ref055","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1002\/jcb.29236","article-title":"RASSF1 promotes cardiomyocyte apoptosis after acute myocardial infarction and is regulated by miR-125b","volume":"121","author":"B Xiaochuan","year":"2020","journal-title":"J Cell 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Sci"},{"issue":"12","key":"pone.0348180.ref059","doi-asserted-by":"crossref","first-page":"2851","DOI":"10.1080\/15548627.2022.2052460","article-title":"Endosomal-associated RFFL facilitates mitochondrial clearance by enhancing PRKN\/parkin recruitment to mitochondria","volume":"18","author":"R Ravindran","year":"2022","journal-title":"Autophagy"},{"issue":"12","key":"pone.0348180.ref060","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.263830","article-title":"Endosomal RFFL ubiquitin ligase regulates mitochondrial morphology by targeting mitofusin 2","volume":"138","author":"ND Narendradev","year":"2025","journal-title":"J Cell Sci"},{"issue":"10","key":"pone.0348180.ref061","doi-asserted-by":"crossref","first-page":"2804","DOI":"10.1128\/MCB.01667-08","article-title":"Zinc finger protein Zbtb20 is essential for postnatal survival and glucose homeostasis","volume":"29","author":"APR Sutherland","year":"2009","journal-title":"Mol Cell 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