{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T18:12:05Z","timestamp":1778177525135,"version":"3.51.4"},"reference-count":15,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:00:00Z","timestamp":1778112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013238","name":"Faculty of Medicine Siriraj Hospital, Mahidol University","doi-asserted-by":"publisher","award":["R016831006"],"award-info":[{"award-number":["R016831006"]}],"id":[{"id":"10.13039\/501100013238","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"\n Background<\/jats:title>\n Hemoglobin (Hb) assessment is crucial to prevent blood donation from anemic donors. Most measurements are invasive and painful; however, an alternative, non-invasive hemoglobin measurement is available. This study aimed to compare non-invasive and point-of-care invasive hemoglobin test results with those from an automated blood analyzer in blood donors.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Qualified blood donors were enrolled at a tertiary care hospital in Bangkok, Thailand. Hemoglobin was initially measured by the non-invasive device (Rad-67 Pulse CO-Oximeter) and a point-of-care invasive hemoglobin screening device (Mission HemoPro). Participants with point-of-care invasive hemoglobin\u2009\u2265\u200912.5 g\/dL were eligible to donate, and during donation, standard hemoglobin measurements were obtained using an automated analyzer, XN-550. Those who were ineligible with point-of-care invasive hemoglobin\u2009<\u200912.5 g\/dL underwent additional blood sampling for hemoglobin measurement by an automated analyzer. Correlation and agreement between non-invasive, point-of-care, and automated hemoglobin measurements were assessed with Intraclass Correlation Coefficient (ICC) and Bland-Altman plots, while satisfaction was evaluated using a Likert scale.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Of 300 participants, 295 had complete data. Of these, 169 were male (57.28%). Average non-invasive, point-of-care, and automated hemoglobin levels were 14.38\u2009\u00b1\u20091.12 g\/dL, 13.65\u2009\u00b1\u20090.70 g\/dL, and 13.90\u2009\u00b1\u20091.16 g\/dL, respectively. The ICC between non-invasive, point-of-care, and automated hemoglobin measurement was 0.600 (95% CI: 0.522\u20130.668) and 0.897 (95% CI: 0.872\u20130.957). The sensitivity of the non-invasive Hb measurement was poor for detecting anemic donors when the Hb cut-off was set at 12.5 g\/dL. This method received significantly higher satisfaction than the routinely used invasive device.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n The non-invasive Hb measurement in blood donors showed moderate agreement with the standard test, but the sensitivity was poor when the cut-off hemoglobin was set at 12.5 g\/dl. Since donor satisfaction was higher, this method may be used as an alternative screening tool, provided a higher Hb cut-off value is used.<\/jats:p>\n <\/jats:sec>","DOI":"10.1371\/journal.pone.0348447","type":"journal-article","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T17:25:10Z","timestamp":1778174710000},"page":"e0348447","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of non-invasive hemoglobin measurement in blood donors at a tertiary care hospital, Bangkok, Thailand"],"prefix":"10.1371","volume":"21","author":[{"given":"Ratinan","family":"Dangwilailert","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somboon","family":"Lekmak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duangtida","family":"Promlee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanyaporn","family":"Pongkunakorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3318-1744","authenticated-orcid":true,"given":"Parichart","family":"Permpikul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,5,7]]},"reference":[{"key":"pone.0348447.ref001","volume-title":"Blood donor selection: guidelines on assessing donor suitability for blood 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versus venous haemoglobin determination in the assessment of healthy blood donors","volume":"104","author":"AJ Patel","year":"2013","journal-title":"Vox Sang"},{"key":"pone.0348447.ref006","volume-title":"Operator\u2019s manual: Masimo","year":"2021"},{"issue":"2","key":"pone.0348447.ref007","doi-asserted-by":"crossref","first-page":"128","DOI":"10.3390\/diagnostics15020128","article-title":"Evaluation of Non-Invasive Hemoglobin Monitoring in Perioperative Patients: A Retrospective Study of the Rad-67TM (Masimo)","volume":"15","author":"P Helmer","year":"2025","journal-title":"Diagnostics (Basel)"},{"key":"pone.0348447.ref008","doi-asserted-by":"crossref","first-page":"21","DOI":"10.21037\/jeccm-24-13","article-title":"Predictive value of perfusion index (PI) on the discrepancy between oxygen saturation measurement via pulse oximetry and blood gas analysis","volume":"8","author":"L Schneider","year":"2024","journal-title":"J Emerg Crit Care Med"},{"key":"pone.0348447.ref009","doi-asserted-by":"crossref","first-page":"1427261","DOI":"10.3389\/fgwh.2024.1427261","article-title":"Diagnostic accuracy of a non-invasive spot-check hemoglobin meter, Masimo Rad-67\u00ae pulse CO-Oximeter\u00ae, in detection of anemia in antenatal care settings in Kenya","volume":"5","author":"A Koech","year":"2024","journal-title":"Front Glob Womens Health"},{"issue":"1","key":"pone.0348447.ref010","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0313319","article-title":"Comparison of a non-invasive point-of-care measurement of anemia to conventionally used HemoCue devices in Gambella refugee camp, Ethiopia, 2022","volume":"20","author":"F Smith","year":"2025","journal-title":"PLoS One"},{"issue":"1","key":"pone.0348447.ref011","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0030065","article-title":"Non-invasive measurement of hemoglobin: assessment of two different point-of-care technologies","volume":"7","author":"E Gayat","year":"2012","journal-title":"PLoS One"},{"issue":"4","key":"pone.0348447.ref012","doi-asserted-by":"crossref","first-page":"261","DOI":"10.3343\/alm.2013.33.4.261","article-title":"Comparison of the accuracy of noninvasive hemoglobin sensor (NBM-200) and portable hemoglobinometer (HemoCue) with an automated hematology analyzer (LH500) in blood donor screening","volume":"33","author":"MJ Kim","year":"2013","journal-title":"Ann Lab Med"},{"issue":"3","key":"pone.0348447.ref013","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1097\/ALN.0b013e3182270c22","article-title":"Comparison of the accuracy of noninvasive hemoglobin monitoring by spectrophotometry (SpHb) and HemoCue\u00ae with automated laboratory hemoglobin measurement","volume":"115","author":"L Lamhaut","year":"2011","journal-title":"Anesthesiology"},{"issue":"2","key":"pone.0348447.ref014","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.jcm.2016.02.012","article-title":"A Guideline of 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