{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T06:02:06Z","timestamp":1781762526584,"version":"3.54.5"},"reference-count":69,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Chang zheng hospital","award":["BSCX2024-08"],"award-info":[{"award-number":["BSCX2024-08"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The purpose of this study is to identify hub genes associated with both osteoporosis (OP) and chronic kidney disease (CKD) through bioinformatics analysis, and to explore the potential pathogenetic mechanisms in OP and CKD through these hub genes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We downloaded the GSE15072 and GSE56815 datasets from the GEO database as training sets, and GSE7158 and GSE70528 for validation. Differential expression genes were selected using the \u201climma\u201d package, while gene co-expression networks were constructed with \u201cWGCNA.\u201d Functional enrichment analyses were performed using \u201cclusterProfiler.\u201d Hub genes were identified through machine learning techniques, and their diagnostic efficacy was evaluated by ROC curves plotted with the \u2018pROC\u2019 package. Immune infiltration was analyzed using CIBERSORT, and pan-cancer relationships were explored to identify associations between hub genes and various tumors. Potential therapeutic agents were investigated using the Drug Signatures Database (DSigDB). Experimental validation was conducted via RT-qPCR using cisplatin-induced chronic kidney disease (CKD) and ovariectomy (OVX)-induced osteoporosis models in C57BL\/6J mice. After anesthesia and sacrifice, peripheral blood mononuclear cells (PBMCs) were collected to analyze the expression changes of hub genes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>This study identified four hub genes (FAM184A, NFKBIA, RP2, HIRA). All hub genes exhibited excellent diagnostic performance, with FAM184A showing the best performance. Immune infiltration analysis revealed the relationships between hub gene expression levels and various immune cells. Pan-cancer analysis revealed the expression levels of FAM184A in different tumors, and it showed that high expression of FAM184A in SARC, SKCM, and PAAD is associated with improved prognosis and reduced mortality rates. Finally, RT-qPCR analysis revealed the mRNA expression levels of the hub genes in both OP and CKD. The mRNA expression of all hub genes were downregulated in osteoporosis model mice compared with normal mice, while in CKD mice, the mRNA expression of all hub genes except FAM184A was upregulated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study identified four hub genes with significant diagnostic efficacy, suggesting they may act as crucial links between osteoporosis and chronic kidney disease. These genes offer promising targets for the treatment of both diseases. The findings of this study provide valuable insights for future research, which could further elucidate the complex pathogenetic mechanisms connecting chronic kidney disease and osteoporosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0348515","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T17:35:03Z","timestamp":1777916103000},"page":"e0348515","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification of potential biomarkers for osteoporosis and chronic kidney disease through bioinformatics and machine learning 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Compston","year":"2019","journal-title":"Lancet"},{"issue":"8","key":"pone.0348515.ref002","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1007\/s001980070064","article-title":"Long-term risk of osteoporotic fracture in Malm\u00f6","volume":"11","author":"JA Kanis","year":"2000","journal-title":"Osteoporos Int"},{"issue":"6","key":"pone.0348515.ref003","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/0002-9343(93)90218-E","article-title":"Consensus development conference: diagnosis, prophylaxis, and treatment of osteoporosis","volume":"94","year":"1993","journal-title":"Am J Med"},{"issue":"9773","key":"pone.0348515.ref004","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1016\/S0140-6736(10)62349-5","article-title":"Osteoporosis: now and the future","volume":"377","author":"TD Rachner","year":"2011","journal-title":"Lancet"},{"issue":"9156","key":"pone.0348515.ref005","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1016\/S0140-6736(98)09075-8","article-title":"Mortality after all major types of osteoporotic fracture in men and women: an observational study","volume":"353","author":"JR Center","year":"1999","journal-title":"Lancet"},{"issue":"10302","key":"pone.0348515.ref006","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1016\/S0140-6736(21)00519-5","article-title":"Chronic kidney disease","volume":"398","author":"K Kalantar-Zadeh","year":"2021","journal-title":"Lancet"},{"issue":"10225","key":"pone.0348515.ref007","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/S0140-6736(20)30045-3","article-title":"Global, regional, and national burden of chronic kidney disease, 1990-2017: a systematic analysis for the Global Burden of Disease Study 2017","volume":"395","author":"GBD Chronic Kidney Disease Collaboration","year":"2020","journal-title":"Lancet"},{"issue":"4","key":"pone.0348515.ref008","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S0272-6386(96)90461-8","article-title":"Prevalence and risk factors for osteopenia in dialysis patients","volume":"28","author":"MS Stein","year":"1996","journal-title":"Am J Kidney Dis"},{"issue":"7","key":"pone.0348515.ref009","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.2215\/CJN.09470913","article-title":"Bone mineral density and serum biochemical predictors of bone loss in patients with CKD on dialysis","volume":"9","author":"HH Malluche","year":"2014","journal-title":"Clin J Am Soc Nephrol"},{"issue":"4","key":"pone.0348515.ref010","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1097\/00041552-199707000-00005","article-title":"Role of cytokines in renal osteodystrophy","volume":"6","author":"MC Monier-Faugere","year":"1997","journal-title":"Curr Opin Nephrol Hypertens"},{"issue":"1","key":"pone.0348515.ref011","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1053\/ajkd.2001.20574","article-title":"Cultures of human osteoblastic cells from dialysis patients: influence of bone turnover rate on in vitro selection of interleukin-6 and osteoblastic cell makers","volume":"37","author":"MC S\u00e1nchez","year":"2001","journal-title":"Am J Kidney Dis"},{"issue":"1","key":"pone.0348515.ref012","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1023\/A:1020146023812","article-title":"Interleukin-6 induced suppression of bovine parathyroid hormone secretion","volume":"19","author":"E Carlstedt","year":"1999","journal-title":"Biosci Rep"},{"issue":"3","key":"pone.0348515.ref013","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1006\/bbrc.1997.7207","article-title":"Inhibition of PTH secretion by interleukin-1 beta in bovine parathyroid glands in vitro is associated with an up-regulation of the calcium-sensing receptor 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Ruiz-Ortega","year":"2020","journal-title":"Nat Rev Nephrol"},{"issue":"4","key":"pone.0348515.ref017","doi-asserted-by":"crossref","first-page":"227","DOI":"10.3390\/toxins12040227","article-title":"Inflammation and Premature Ageing in Chronic Kidney Disease","volume":"12","author":"T Ebert","year":"2020","journal-title":"Toxins (Basel)"},{"key":"pone.0348515.ref018","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.cca.2020.03.040","article-title":"Bone, inflammation and chronic kidney disease","volume":"506","author":"S Mazzaferro","year":"2020","journal-title":"Clin Chim Acta"},{"key":"pone.0348515.ref019","doi-asserted-by":"crossref","first-page":"822178","DOI":"10.3389\/fphar.2022.822178","article-title":"Efficacy of Osteoporosis Medications for Patients With Chronic Kidney Disease: An Updated Systematic Review and Network Meta-Analysis","volume":"13","author":"C-H Chen","year":"2022","journal-title":"Front Pharmacol"},{"issue":"4","key":"pone.0348515.ref020","article-title":"Osteoporosis and chronic kidney disease: review and new therapeutic strategies","volume":"36","author":"C Sessa","year":"2019","journal-title":"G Ital Nefrol"},{"key":"pone.0348515.ref021","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1186\/1471-2105-9-559","article-title":"WGCNA: an R package for weighted correlation network analysis","volume":"9","author":"P Langfelder","year":"2008","journal-title":"BMC Bioinformatics"},{"issue":"1","key":"pone.0348515.ref022","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1038\/s41580-021-00407-0","article-title":"A guide to machine learning for biologists","volume":"23","author":"JG Greener","year":"2022","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"7","key":"pone.0348515.ref023","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1038\/s41587-019-0114-2","article-title":"Determining cell type abundance and expression from bulk tissues with 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Tasnim","year":"2021","journal-title":"Cureus"},{"issue":"5","key":"pone.0348515.ref027","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1016\/j.kint.2023.08.009","article-title":"All the might of the osteocyte: emerging roles in chronic kidney disease","volume":"104","author":"LV Albrecht","year":"2023","journal-title":"Kidney Int"},{"issue":"11","key":"pone.0348515.ref028","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1038\/ki.2008.356","article-title":"Bisphosphonate nephrotoxicity","volume":"74","author":"MA Perazella","year":"2008","journal-title":"Kidney Int"},{"issue":"3","key":"pone.0348515.ref029","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1053\/j.ajkd.2021.06.031","article-title":"Diagnosis and Management of Osteoporosis in Advanced Kidney Disease: A Review","volume":"79","author":"C Ginsberg","year":"2022","journal-title":"Am J Kidney 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Dis"},{"issue":"7","key":"pone.0348515.ref033","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1056\/NEJMoa1006312","article-title":"NFKBIA deletion in glioblastomas","volume":"364","author":"M Bredel","year":"2011","journal-title":"N Engl J Med"},{"issue":"12","key":"pone.0348515.ref034","doi-asserted-by":"crossref","first-page":"2974","DOI":"10.3390\/ijms20122974","article-title":"Protective Effect of Acteoside on Ovariectomy-Induced Bone Loss in Mice","volume":"20","author":"L Yang","year":"2019","journal-title":"Int J Mol Sci"},{"issue":"12","key":"pone.0348515.ref035","doi-asserted-by":"crossref","first-page":"10229","DOI":"10.3390\/ijms241210229","article-title":"Lost and Found: The Family of NF-\u03baB Inhibitors Is Larger than Assumed in Salmonid Fish","volume":"24","author":"DR van Muilekom","year":"2023","journal-title":"Int J Mol Sci"},{"issue":"11","key":"pone.0348515.ref036","doi-asserted-by":"crossref","first-page":"5107","DOI":"10.1167\/iovs.08-3208","article-title":"Identification of an intronic single-point mutation in RP2 as the cause of semidominant X-linked retinitis pigmentosa","volume":"50","author":"E Pomares","year":"2009","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"5","key":"pone.0348515.ref037","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1042\/BST20160148","article-title":"Disease mechanisms of X-linked retinitis pigmentosa due to RP2 and RPGR mutations","volume":"44","author":"R Lyraki","year":"2016","journal-title":"Biochem Soc Trans"},{"key":"pone.0348515.ref038","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.visres.2012.07.016","article-title":"Arl3 and RP2 mediated assembly and traffic of membrane associated cilia proteins","volume":"75","author":"N Schwarz","year":"2012","journal-title":"Vision Res"},{"issue":"13","key":"pone.0348515.ref039","doi-asserted-by":"crossref","first-page":"2480","DOI":"10.1093\/hmg\/ddx143","article-title":"Arl3 and RP2 regulate the trafficking of ciliary tip kinesins","volume":"26","author":"N Schwarz","year":"2017","journal-title":"Hum Mol Genet"},{"issue":"22","key":"pone.0348515.ref040","doi-asserted-by":"crossref","first-page":"4330","DOI":"10.1093\/hmg\/ddq355","article-title":"The retinitis pigmentosa protein RP2 interacts with polycystin 2 and regulates cilia-mediated vertebrate development","volume":"19","author":"T Hurd","year":"2010","journal-title":"Hum Mol Genet"},{"issue":"4","key":"pone.0348515.ref041","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/s41581-019-0116-9","article-title":"Cellular signalling by primary cilia in development, organ function and disease","volume":"15","author":"Z Anvarian","year":"2019","journal-title":"Nat Rev Nephrol"},{"issue":"3","key":"pone.0348515.ref042","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1177\/00220345211046606","article-title":"Role of Primary Cilia in Bone and Cartilage","volume":"101","author":"Z Chinipardaz","year":"2022","journal-title":"J Dent Res"},{"issue":"4","key":"pone.0348515.ref043","doi-asserted-by":"crossref","DOI":"10.1002\/jbm4.10464","article-title":"Characterization of Primary Cilia in Osteoblasts Isolated From Patients With ADPKD and CKD","volume":"5","author":"RC Pereira","year":"2021","journal-title":"JBMR Plus"},{"issue":"7","key":"pone.0348515.ref044","doi-asserted-by":"crossref","first-page":"2136","DOI":"10.1016\/j.celrep.2020.01.062","article-title":"HIRA, a DiGeorge Syndrome Candidate Gene, Confers Proper Chromatin Accessibility on HSCs and Supports All Stages of Hematopoiesis","volume":"30","author":"C Chen","year":"2020","journal-title":"Cell Rep"},{"issue":"23","key":"pone.0348515.ref045","article-title":"O-linked N-acetylglucosamine transferase (OGT) interacts with the histone chaperone HIRA complex and regulates nucleosome assembly and cellular senescence","volume":"113","author":"J-S Lee","year":"2016","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"24","key":"pone.0348515.ref046","doi-asserted-by":"crossref","first-page":"2712","DOI":"10.1101\/gad.247528.114","article-title":"HIRA orchestrates a dynamic chromatin landscape in senescence and is required for suppression of neoplasia","volume":"28","author":"TS Rai","year":"2014","journal-title":"Genes Dev"},{"issue":"12","key":"pone.0348515.ref047","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1038\/nrneph.2011.149","article-title":"Cellular and molecular mechanisms of renal fibrosis","volume":"7","author":"Y Liu","year":"2011","journal-title":"Nat Rev Nephrol"},{"issue":"1","key":"pone.0348515.ref048","doi-asserted-by":"crossref","first-page":"14060","DOI":"10.1038\/s41598-018-32518-8","article-title":"TGF-\u03b21 promotes expression of fibrosis-related genes through the induction of histone variant H3.3 and histone chaperone HIRA","volume":"8","author":"T Shindo","year":"2018","journal-title":"Sci Rep"},{"key":"pone.0348515.ref049","doi-asserted-by":"crossref","first-page":"567931","DOI":"10.3389\/fonc.2020.567931","article-title":"A Ten-N6-Methyladenosine (m6A)-Modified Gene Signature Based on a Risk Score System Predicts Patient Prognosis in Rectum Adenocarcinoma","volume":"10","author":"W Huang","year":"2021","journal-title":"Front Oncol"},{"key":"pone.0348515.ref050","doi-asserted-by":"crossref","first-page":"373","DOI":"10.3389\/fgene.2019.00373","article-title":"Identification of Potential Crucial Genes Associated With the Pathogenesis and Prognosis of Endometrial Cancer","volume":"10","author":"L Liu","year":"2019","journal-title":"Front Genet"},{"key":"pone.0348515.ref051","doi-asserted-by":"crossref","first-page":"465","DOI":"10.3389\/fendo.2020.00465","article-title":"Osteoimmunology: The Regulatory Roles of T Lymphocytes in Osteoporosis","volume":"11","author":"W Zhang","year":"2020","journal-title":"Front Endocrinol (Lausanne)"},{"key":"pone.0348515.ref052","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.semcdb.2021.05.014","article-title":"Interaction between bone and immune cells: Implications for postmenopausal osteoporosis","volume":"123","author":"V Fischer","year":"2022","journal-title":"Semin Cell Dev Biol"},{"key":"pone.0348515.ref053","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.2147\/JIR.S351918","article-title":"The Rising Era of \u201cImmunoporosis\u201d: Role of Immune System in the Pathophysiology of Osteoporosis","volume":"15","author":"RK Srivastava","year":"2022","journal-title":"J Inflamm Res"},{"key":"pone.0348515.ref054","doi-asserted-by":"crossref","first-page":"965258","DOI":"10.3389\/fendo.2022.965258","article-title":"Immunoporosis: Role of immune system in the pathophysiology of different types of osteoporosis","volume":"13","author":"W Zhang","year":"2022","journal-title":"Front Endocrinol (Lausanne)"},{"issue":"8","key":"pone.0348515.ref055","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1182\/blood-2008-09-178301","article-title":"Surface RANKL of Toll-like receptor 4-stimulated human neutrophils activates osteoclastic bone resorption","volume":"114","author":"A Chakravarti","year":"2009","journal-title":"Blood"},{"key":"pone.0348515.ref056","doi-asserted-by":"crossref","DOI":"10.3390\/nu12102999","article-title":"Macrophage polarization and osteoporosis: a review","volume":"12","author":"J Mu\u00f1oz","year":"2020","journal-title":"Nutrients"},{"issue":"4","key":"pone.0348515.ref057","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.gendis.2020.03.009","article-title":"The role of dendritic cells derived osteoclasts in bone destruction diseases","volume":"8","author":"B Wang","year":"2020","journal-title":"Genes Dis"},{"key":"pone.0348515.ref058","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.3389\/fimmu.2018.01508","article-title":"Interferon-gamma-mediated osteoimmunology","volume":"9","author":"M Tang","year":"2018","journal-title":"Front Immunol"},{"issue":"6812","key":"pone.0348515.ref059","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1038\/35046102","article-title":"T-cell-mediated regulation of osteoclastogenesis by signalling cross-talk between RANKL and IFN-gamma","volume":"408","author":"H Takayanagi","year":"2000","journal-title":"Nature"},{"issue":"3","key":"pone.0348515.ref060","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.bone.2010.04.611","article-title":"T cells: critical bone regulators in health and disease","volume":"47","author":"R Pacifici","year":"2010","journal-title":"Bone"},{"issue":"5","key":"pone.0348515.ref061","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1186\/ar2502","article-title":"Immune regulation of bone loss by Th17 cells","volume":"10","author":"IE Adamopoulos","year":"2008","journal-title":"Arthritis Res Ther"},{"key":"pone.0348515.ref062","first-page":"17","article-title":"Cancer statistics, 2023","volume":"73","author":"RL Siegel","year":"2023","journal-title":"CA Cancer J Clin"},{"key":"pone.0348515.ref063","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1097\/CM9.0000000000002108","article-title":"Cancer statistics in China and United States, 2022: profiles, trends, and determinants","volume":"135","author":"C Xia","year":"2022","journal-title":"Chin Med J (Engl)"},{"issue":"10033","key":"pone.0348515.ref064","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1016\/S0140-6736(15)00516-4","article-title":"The World report on ageing and health: a policy framework for healthy ageing","volume":"387","author":"JR 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