{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:50:29Z","timestamp":1792788629034,"version":"4.5.0"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Henan Provincial Key Scientific Research Project Plan for Higher Education Institutions","award":["23A320019"],"award-info":[{"award-number":["23A320019"]}]},{"DOI":"10.13039\/100014473","name":"Shandong Provincial Education Science Plan","doi-asserted-by":"publisher","award":["23A320019"],"award-info":[{"award-number":["23A320019"]}],"id":[{"id":"10.13039\/100014473","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Henan Provincial Medical Science and Technology Research Joint Venture Project","award":["SBGJ202402040"],"award-info":[{"award-number":["SBGJ202402040"]}]},{"name":"the Henan Provincial Medical Science and Technology Research Joint Venture Project","award":["SBGJ202402040"],"award-info":[{"award-number":["SBGJ202402040"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82300850"],"award-info":[{"award-number":["82300850"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82300850"],"award-info":[{"award-number":["82300850"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004761","name":"the Natural Science Foundation of Henan Province","doi-asserted-by":"crossref","award":["222300420335"],"award-info":[{"award-number":["222300420335"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004761","name":"the Natural Science Foundation of Henan Province","doi-asserted-by":"crossref","award":["222300420335"],"award-info":[{"award-number":["222300420335"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    This study aimed to explore the mechanisms by which Astragaloside IV (AS-IV), a major bioactive component of\n                    <jats:italic>Astragalus membranaceus<\/jats:italic>\n                    , mitigates high-glucose-induced peritoneal fibrosis (PF) in peritoneal dialysis (PD). Using both in vivo (uremic rat model) and in vitro (human peritoneal mesothelial cells) approaches, we observed that AS-IV treatment was associated with a significant attenuation of PF. This effect was mediated through the inhibition of epithelial-mesenchymal transition (EMT) and fibrosis. In vivo, AS-IV reduced extracellular matrix deposition and collagen accumulation, downregulated EMT and fibrosis markers (\u03b1-SMA, collagen IV), and restored E-cadherin levels. Notably, these changes correlated with the downregulation of ENKUR, pPI3K, and pAkt. The in vitro results corroborated these findings, showing that AS-IV suppressed EMT without cytotoxic effects. Our data indicate that AS-IV may exert antifibrotic effects via modulation of the ENKUR\/PI3K\/Akt signaling pathway, suggesting a potential target for the prevention of PF.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0348762","type":"journal-article","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T23:11:52Z","timestamp":1778281912000},"page":"e0348762","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Astragaloside IV attenuates high-glucose-Induced peritoneal fibrosis via modulation of the ENKUR\/PI3K\/Akt signalling pathway"],"prefix":"10.1371","volume":"21","author":[{"given":"Tianxin","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanzheng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9781-0686","authenticated-orcid":true,"given":"Jing","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,5,8]]},"reference":[{"issue":"9","key":"pone.0348762.ref001","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202403230","article-title":"Kidney 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