{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T18:11:42Z","timestamp":1778523102422,"version":"3.51.4"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["23K21287"],"award-info":[{"award-number":["23K21287"]}]},{"DOI":"10.13039\/501100012359","name":"Promotion and Mutual Aid Corporation for Private Schools of Japan","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100012359","id-type":"DOI","asserted-by":"publisher"}]},{"name":"JSPS KAKENHI","award":["25K23678"],"award-info":[{"award-number":["25K23678"]}]},{"DOI":"10.13039\/501100002871","name":"Chubu University","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100002871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Chromosomal rearrangements generated by CRISPR\/Cas systems are valuable for studying genomic architecture and repair mechanisms. However, most\n                    <jats:italic>in vivo<\/jats:italic>\n                    approaches rely on viral vectors, which require specialised production, prolonged nuclease expression, and elevated biosafety containment. Here, we applied Cas9 ribonucleoprotein (RNP) electroporation to the murine uterine epithelium as a simple, non-viral strategy for somatic chromosomal engineering. This method successfully induced defined interchromosomal translocations at multiple loci and enabled the molecular assessment of large-scale inversion repair (57.8 Mb) using paired gRNAs with an ssODN donor. While rearranged alleles were detected at low apparent frequencies in bulk uterine DNA\u2014consistent with epithelial-restricted delivery and somatic mosaicism\u2014high-depth whole-genome sequencing (WGS) and PCR provided nucleotide-resolution confirmation of precise junction formation. Our findings demonstrate that uterine electroporation of CRISPR RNPs is a feasible, rapid approach for evaluating engineered chromosomal rearrangements\n                    <jats:italic>in vivo<\/jats:italic>\n                    , providing a controlled platform for analyzing somatic DNA repair outcomes without viral confounds.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0348797","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T17:28:01Z","timestamp":1778520481000},"page":"e0348797","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Non-viral in vivo electroporation-based chromosomal engineering and repair assessment in the murine uterine epithelium"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2306-3381","authenticated-orcid":true,"given":"Satoru","family":"Iwata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumi","family":"Miura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Iwamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,5,11]]},"reference":[{"issue":"4","key":"pone.0348797.ref001","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1038\/nrc2091","article-title":"The impact of translocations and gene fusions on cancer causation","volume":"7","author":"F Mitelman","year":"2007","journal-title":"Nat Rev Cancer"},{"issue":"4","key":"pone.0348797.ref002","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.semcancer.2010.05.007","article-title":"Genomic rearrangements in inherited disease and cancer","volume":"20","author":"J-M Chen","year":"2010","journal-title":"Semin Cancer Biol"},{"issue":"1","key":"pone.0348797.ref003","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.neuron.2006.09.027","article-title":"Genomic rearrangements and gene copy-number alterations as a cause of nervous system disorders","volume":"52","author":"JA Lee","year":"2006","journal-title":"Neuron"},{"issue":"2","key":"pone.0348797.ref004","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/gm132","article-title":"Inversion variants in the human genome: role in disease and genome architecture","volume":"2","author":"L Feuk","year":"2010","journal-title":"Genome 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