{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T17:57:44Z","timestamp":1791482264399,"version":"4.3.4"},"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Hydrangea ringspot virus (HdRSV) is an emerging plant virus infecting ornamental hydrangea species worldwide, yet its genomic diversity and host associations remain poorly understood. To expand the available genomic resources and assess HdRSV variability, we screened 210 publicly available\n                    <jats:italic>Hydrangea spp.<\/jats:italic>\n                    transcriptomes from diverse tissues, complemented with four newly generated\n                    <jats:italic>H. macrophylla<\/jats:italic>\n                    transcriptomes from Colombia. Viral genomes were assembled from infected samples and analyzed to infer phylogenetic relationships, lineage distribution, and relative viral RNA abundance. Two well-supported phylogenetic lineages (HdRSV-L1 and HdRSV-L2) were recovered from both full-genome and replicase coding sequence (CDS) analyses. HdRSV was detected across all host tissues examined, with the highest median viral loads in roots, followed by stems and leaves.\n                    <jats:italic>H.<\/jats:italic>\n                    <jats:italic>macrophylla<\/jats:italic>\n                    harbored both viral lineages, while\n                    <jats:italic>H. serrata<\/jats:italic>\n                    was exclusively infected by HdRSV-L1. Cultivar-level analysis revealed marked differences in viral abundance, with lineages showing distinct tissue preferences but no co-infection patterns, except in the \u2018Bailer\u2019 cultivar. Comparative analysis of the replicase CDS identified a single lineage-defining nonsynonymous mutation (C1578T; Thr\u2009\u2192\u2009Ile), fixed in 90% of HdRSV-L2 genomes, corresponding to a polar-to-nonpolar amino acid change potentially associated with structural adaptation. Together, these findings provide the most comprehensive overview to date of HdRSV genomic diversity, host and tissue distribution, and molecular variation, offering new insights into the evolution and epidemiology of this understudied plant virus.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0348969","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T17:39:52Z","timestamp":1791481192000},"page":"e0348969","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic diversity, host associations, and tissue tropism of Hydrangea ringspot virus: A global and regional perspective"],"prefix":"10.1371","volume":"21","author":[{"given":"Juliana","family":"Osorio-Marulanda","sequence":"first","affiliation":[{"name":"Centro Nacional de Secuenciaci\u00f3n Gen\u00f3mica - CNSG, Sede de Investigaci\u00f3n Universitaria- SIU, Universidad de Antioquia UdeA","place":["Medell\u00edn, Antioquia, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Lopez-Jimenez","sequence":"additional","affiliation":[{"name":"Centro Nacional de Secuenciaci\u00f3n Gen\u00f3mica - CNSG, Sede de Investigaci\u00f3n Universitaria- SIU, Universidad de Antioquia UdeA","place":["Medell\u00edn, Antioquia, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2578-4609","authenticated-orcid":true,"given":"Juan F.","family":"Alzate","sequence":"additional","affiliation":[{"name":"Centro Nacional de Secuenciaci\u00f3n Gen\u00f3mica - CNSG, Sede de Investigaci\u00f3n Universitaria- SIU, Universidad de Antioquia UdeA","place":["Medell\u00edn, Antioquia, Colombia"]},{"name":"Universidad de Antioquia UdeA","place":["Medell\u00edn, Antioquia, Colombia"],"department":["Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina"]},{"name":"F\u00e1brica de Plantas y Semillas de Antioquia S.A.S. \u2013 FASPLAN","place":["El Carmen de Viboral, Antioquia, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"key":"pone.0348969.ref001","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/B978-0-323-90899-3.00079-3","article-title":"Common and emerging viral pathogens of annual ornamental plants","author":"D Groth-Helms","year":"2024","journal-title":"Viral Diseases of Field and Horticultural Crops. 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