{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T18:00:26Z","timestamp":1780941626783,"version":"3.54.1"},"reference-count":67,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Diabetes poses an utmost threat to human health; significant progress in the discovery of antidiabetic drugs has been made, but their side effects cannot be ignored. As a plant-based alternative, this study explored the pharmacological potential of\n                    <jats:italic>Elaeocarpus angustifolius<\/jats:italic>\n                    Blume (Rudraksha), emphasizing its antioxidant and antidiabetic activities, supported by\n                    <jats:italic>in silico<\/jats:italic>\n                    analysis. Phytochemical screening of the methanol bark extract confirmed the presence of flavonoids, alkaloids, carbohydrates, glycosides, terpenoids, tannins, and phenols. The extract exhibited high total phenolic and flavonoid contents (182.73\u2009\u00b1\u20090.001\u2009mg GAE\/g and 48.78\u2009\u00b1\u20090.06\u2009mg QE\/g, respectively). The LC\u2013MS analysis of the ethyl acetate fraction identified thirteen phytoconstituents, including phenolic acids, flavonoids, and tannins. The ethyl acetate fraction demonstrated significant antioxidant activity (IC\u2085\u2080\u2009=\u20091.47\u2009\u00b1\u20090.37 \u00b5g\/mL), outperforming quercetin (IC\u2085\u2080\u2009=\u20094.87\u2009\u00b1\u20090.16 \u00b5g\/mL). Enzyme inhibition assays revealed methanol extract has strong \u03b1-glucosidase (IC\u2085\u2080\u2009=\u20090.79\u2009\u00b1\u20090.13 \u00b5g\/mL) and \u03b1-amylase (IC\u2085\u2080\u2009=\u20093.48\u2009\u00b1\u20090.00 \u00b5g\/mL) inhibitory effects, comparable to acarbose (IC\u2085\u2080\u2009=\u200913.51\u2009\u00b1\u20090.22 \u00b5g\/mL and IC\u2085\u2080\u2009=\u200925.30\u2009\u00b1\u20090.85 \u00b5g\/mL), respectively. In a 28-day\n                    <jats:italic>in vivo<\/jats:italic>\n                    study, oral administration of the methanol bark extract significantly (p\u2009&lt;\u20090.01) reduced fasting serum glucose levels and improved lipid profiles, with decreases in triglycerides (5%) and total cholesterol (2%), and an increase in HDL (5%) compared with baseline. The extract-treated group also showed higher hepatic glycogen content (14.06\u2009\u00b1\u20090.002\u2009mg\/mL) than the Gliclazide-treated group (12.82\u2009\u00b1\u20090.002\u2009mg\/mL).\n                    <jats:italic>In silico<\/jats:italic>\n                    molecular docking identified Corilagin exhibiting the highest binding affinity (\u221210.3 kcal\/mol), stable interaction (RMSD\u2009=\u20091.469\u2009\u00b1\u20090.133 \u00c5), and favorable binding free energy (\u0394G\n                    <jats:sub>BFE<\/jats:sub>\n                    \u2009=\u2009\u221227.48\u2009\u00b1\u20093.15) with \u03b1-amylase, suggesting that it may act as a competitive inhibitor of an enzyme\u2019s active site. Overall, these findings highlight that\n                    <jats:italic>E. angustifolius<\/jats:italic>\n                    bark significantly inhibits glucose absorption and improves dyslipidemia to some extent, while\n                    <jats:italic>in silico<\/jats:italic>\n                    analysis suggests that Corilagin has potential as an inhibitory activity against type-2 diabetes.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0349796","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T17:31:31Z","timestamp":1780939891000},"page":"e0349796","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biochemical and antidiabetic properties of Elaeocarpus angustifolius Blume: In vitro, In vivo, and In silico insights"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7409-7384","authenticated-orcid":true,"given":"Sabina","family":"Panth","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rojina","family":"Devkota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5783-5089","authenticated-orcid":true,"given":"Amar","family":"Waiba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tika Ram","family":"Lamichhane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prajwal","family":"Acharya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Begum","family":"Rokeya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Jaisi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1065-5727","authenticated-orcid":true,"given":"Achyut","family":"Adhikari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,6,8]]},"reference":[{"issue":"3","key":"pone.0349796.ref001","first-page":"224","article-title":"Diabetes mellitus: a review","volume":"3","author":"CD Deshmukh","year":"2015","journal-title":"Int J Pure App Biosci"},{"issue":"6","key":"pone.0349796.ref002","doi-asserted-by":"crossref","first-page":"110","DOI":"10.4239\/wjd.v3.i6.110","article-title":"Trends in prevalence of diabetes in Asian countries","volume":"3","author":"A Ramachandran","year":"2012","journal-title":"World J Diabetes"},{"key":"pone.0349796.ref003","author":"IDF. 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