{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:25:18Z","timestamp":1787163918705,"version":"build-2736575974"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Atypical respiratory pathogens such as\n                    <jats:italic>Mycoplasma pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Chlamydophila pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    , and\n                    <jats:italic>Bordetella pertussis<\/jats:italic>\n                    are significant contributors to global morbidity and mortality from respiratory infections, and their prevalence has increased in recent years. Their detection is complicated by unique biological features and genetic variability. This study presents a comprehensive post-market surveillance (PMS) framework for the QIAstat-Dx\u00ae Respiratory SARS-CoV-2 Panel, integrating bioinformatic workflows and an AI-powered literature review developed by QIAGEN to monitor assay performance over the last years. Inclusivity and cross-reactivity protocols were applied to assess the impact of genetic mutations on assay sensitivity and specificity. The analysis revealed high inclusivity and specificity of the panel, with only a single potentially critical mutation detected in the transposon-targeted gene at low frequency and expected cross-reactivity among\n                    <jats:italic>Bordetella<\/jats:italic>\n                    species. The AI-driven tool enhanced the surveillance process, especially for pathogens with limited sequence data. The findings support the continued reliability of the QIAstat-Dx\u00ae Respiratory SARS-CoV-2 Panel for diagnosing atypical respiratory bacterial infections and highlight the importance of ongoing molecular surveillance using advanced bioinformatics and AI technologies.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0349967","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:35:52Z","timestamp":1787160952000},"page":"e0349967","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comprehensive and innovative post-market surveillance system for atypical respiratory pathogens detecting qPCR panel"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4709-5556","authenticated-orcid":true,"given":"Barbara","family":"Kosi\u0144ska-Selbi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justyna","family":"Kowalczyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jagoda","family":"Pier\u015bci\u0144ska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0587-8990","authenticated-orcid":true,"given":"Jaros\u0142aw","family":"We\u0142eszczuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Pe\u00f1arrubia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0187-6066","authenticated-orcid":true,"given":"Joanna","family":"Reniewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinay","family":"Suryaprakash","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rubi","family":"Diaz-Hernandez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mart\u00ed","family":"Juanola-Falgarona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Blacha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"issue":"1","key":"pone.0349967.ref001","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.resinv.2021.09.009","article-title":"Atypical pneumonia: pathophysiology, diagnosis, and treatment","volume":"60","author":"N Miyashita","year":"2022","journal-title":"Respir Investig"},{"issue":"5","key":"pone.0349967.ref002","doi-asserted-by":"crossref","first-page":"424","DOI":"10.3892\/etm.2024.12713","article-title":"Atypical pneumonia (Review)","volume":"28","author":"VE Georgakopoulou","year":"2024","journal-title":"Exp Ther Med"},{"issue":"12","key":"pone.0349967.ref003","doi-asserted-by":"crossref","first-page":"2326","DOI":"10.3390\/microorganisms10122326","article-title":"Atypical pathogens in adult community-acquired pneumonia and implications for empiric antibiotic treatment: a narrative review","volume":"10","author":"N Garin","year":"2022","journal-title":"Microorganisms"},{"issue":"11","key":"pone.0349967.ref004","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1016\/S1473-3099(22)00510-2","article-title":"Age-sex differences in the global burden of lower respiratory infections and risk factors, 1990\u20132019: results from the Global Burden of Disease Study 2019","volume":"22","author":"GBD 2019 LRI Collaborators","year":"2022","journal-title":"Lancet Infect Dis"},{"issue":"6","key":"pone.0349967.ref005","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1370\/afm.1993","article-title":"Prevalence of atypical pathogens in patients with cough and community-acquired pneumonia: a meta-analysis","volume":"14","author":"C Marchello","year":"2016","journal-title":"Ann Fam Med"},{"key":"pone.0349967.ref006","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s12952-016-0047-y","article-title":"Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria","volume":"15","author":"M Herrera","year":"2016","journal-title":"J Negat Results Biomed"},{"key":"pone.0349967.ref007","unstructured":"Juanola\u2011Falgarona M, Miravet C, Pau L. Respiratory epidemiological surveillance through a rapid syndromic testing platform shows surge in bacterial upper respiratory tract infections in Denmark during 2024. Abstract presented at: ESCMID Global; 2024. [Internet]. 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