{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T18:06:21Z","timestamp":1779473181951,"version":"3.53.1"},"reference-count":29,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To identify key genes and shared pathogenic pathways associated with acute pancreatitis-associated acute lung injury (AP-ALI), through bioinformatics analysis, and to provide potential molecular targets for the diagnosis and treatment of AP-ALI.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The cerulein-induced severe acute pancreatitis (SAP) mouse lung tissue dataset (GSE244335), lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse lung tissue dataset (GSE216943), and human AP patient peripheral blood dataset (GSE194331) were retrieved from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were screened. Subsequently, we conducted a series of bioinformatic analyses, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and protein-protein interaction (PPI) network construction. The PPI network was constructed and hub genes were screened. Cross-species consistency of key pathways was verified using the human dataset.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 469 and 153 DEGs were screened from GSE244335 and GSE216943, respectively, with 94 overlapping common DEGs. GO\/KEGG enrichment analyses showed that these common DEGs were mainly enriched in immune-inflammatory responses, chemokine receptor binding, and NF-\u03baB signaling pathways. PPI network analysis identified the top 10 hub genes in mice (IL-1\u03b2, CCL3, CXCL2, CXCL10, CCL2, CXCL9, CXCL1, CXCR2, TLR2, TNF). Ten hub genes (S100A9, ARG1, RETN, etc.) were screened from the human AP dataset (GSE194331). Cross-species comparison of mouse lung tissue and human peripheral blood revealed 6 common GO-BP terms related to systemic inflammatory responses, suggesting shared mechanisms between local lung injury and systemic inflammation in AP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The identified hub genes (e.g., IL-1\u03b2, CXCL2) and the IL-17\/NF-\u03baB signaling pathway represent candidate molecular signatures implicated in AP-ALI. These computational findings generate testable hypotheses regarding inflammatory mechanisms, potentially highlighting shared systemic inflammatory processes between mouse lung injury and human peripheral blood. Direct validation in human pulmonary tissue or bronchoalveolar lavage fluid is warranted to confirm their local pathogenic relevance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0350013","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T17:34:16Z","timestamp":1779471256000},"page":"e0350013","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of key genes and signaling pathways associated with acute pancreatitis and acute lung injury by bioinformatics 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