{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T17:51:16Z","timestamp":1782150676148,"version":"3.54.5"},"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>This systematic review examined the use of surface electromyography (sEMG) for the neuromuscular assessment of individuals with Amyotrophic Lateral Sclerosis (ALS), focusing on clinical parameters, the muscle groups evaluated, acquisition protocols, technical properties of the recording systems, integration with other technologies, and signal processing strategies. We included observational studies that applied sEMG to individuals diagnosed with ALS, with or without comparison to healthy controls, and without restrictions on publication year. The analyses included signals recorded at rest and during voluntary contractions, with or without the use of biofeedback. Most studies employed conventional or high-density surface electrodes, with sampling frequencies ranging from 500 Hz to 3000 Hz. The results showed that the primary parameters assessed were muscle fatigue, fasciculation patterns, the number of motor units (MUNE\/MUNIX), motor unit firing rates, and signal complexity. These parameters demonstrated sensitivity to disease progression and may contribute to early diagnosis, phenotypic stratification, and functional monitoring of ALS. Additionally, the studies highlighted the increasing use of advanced computational approaches, such as machine learning, for feature extraction and automated classification. In conclusion, sEMG is a promising tool for functional assessment in ALS, with the potential to improve diagnostic accuracy and support new therapeutic strategies based on electrophysiological biomarkers. However, despite technological advances, the included studies displayed substantial methodological heterogeneity and limited protocol standardization. Integration with other neurophysiological modalities also remains underexplored, despite its significant clinical potential.<\/jats:p>","DOI":"10.1371\/journal.pone.0350029","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T17:34:02Z","timestamp":1782149642000},"page":"e0350029","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Applications of electromyography in Amyotrophic Lateral Sclerosis: A systematic review"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0100-8026","authenticated-orcid":true,"given":"Ana Paula Mendon\u00e7a","family":"Fernandes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Henrique","family":"Bertucci Borges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ledycnarf Janu\u00e1rio de","family":"Holanda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6713-4738","authenticated-orcid":true,"given":"Bruno Henrique e Silva","family":"Bezerra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3792-3794","authenticated-orcid":true,"given":"Anna Clara Sales Miranda","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Clara Fernandes da","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9216-8593","authenticated-orcid":true,"given":"Ricardo A. de M.","family":"Valentim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Bougrain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Alves Mendes","family":"Vasiljevic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9628-7891","authenticated-orcid":true,"given":"Ana Raquel","family":"Rodrigues Lindquist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,6,22]]},"reference":[{"key":"pone.0350029.ref001","doi-asserted-by":"crossref","first-page":"17071","DOI":"10.1038\/nrdp.2017.71","article-title":"Amyotrophic lateral sclerosis","volume":"3","author":"O Hardiman","year":"2017","journal-title":"Nat Rev Dis Primers"},{"issue":"10","key":"pone.0350029.ref002","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1212\/WNL.0b013e3181b6bbbd","article-title":"ALS motor phenotype heterogeneity, focality, and spread: deconstructing motor neuron degeneration","volume":"73","author":"JM Ravits","year":"2009","journal-title":"Neurology"},{"issue":"2","key":"pone.0350029.ref003","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.chest.2018.06.035","article-title":"Respiratory failure in amyotrophic lateral sclerosis","volume":"155","author":"S Niedermeyer","year":"2019","journal-title":"Chest"},{"issue":"5","key":"pone.0350029.ref004","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.neurol.2017.03.031","article-title":"Care management in amyotrophic lateral sclerosis","volume":"173","author":"M-H Soriani","year":"2017","journal-title":"Rev Neurol (Paris)"},{"key":"pone.0350029.ref005","unstructured":"Konrad P. 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Bashford","year":"2020","journal-title":"Brain Commun"},{"issue":"6","key":"pone.0350029.ref048","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1002\/mus.26864","article-title":"Fasciculations demonstrate daytime consistency in amyotrophic lateral sclerosis","volume":"61","author":"J Bashford","year":"2020","journal-title":"Muscle Nerve"},{"issue":"3","key":"pone.0350029.ref049","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1002\/mus.27139","article-title":"Fasciculation analysis reveals a novel parameter that correlates with predicted survival in amyotrophic lateral sclerosis","volume":"63","author":"K Wannop","year":"2021","journal-title":"Muscle Nerve"},{"issue":"17","key":"pone.0350029.ref050","doi-asserted-by":"crossref","first-page":"4117","DOI":"10.1113\/JP281310","article-title":"First-recruited motor units adopt a faster phenotype in amyotrophic lateral sclerosis","volume":"599","author":"T Weddell","year":"2021","journal-title":"J Physiol"},{"key":"pone.0350029.ref051","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.clinph.2022.11.005","article-title":"Fasciculation electromechanical latency is prolonged in amyotrophic lateral sclerosis","volume":"145","author":"D Planinc","year":"2023","journal-title":"Clin Neurophysiol"},{"issue":"5","key":"pone.0350029.ref052","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.neucli.2021.06.001","article-title":"Surface electromyography for testing motor dysfunction in amyotrophic lateral sclerosis","volume":"51","author":"C Quint\u00e3o","year":"2021","journal-title":"Neurophysiol Clin"},{"key":"pone.0350029.ref053","doi-asserted-by":"crossref","first-page":"104011","DOI":"10.1016\/j.bspc.2022.104011","article-title":"A morphology-based feature set for automated Amyotrophic Lateral Sclerosis diagnosis on surface electromyography","volume":"79","author":"M Antunes","year":"2023","journal-title":"Biomed Signal Process Control"},{"issue":"4","key":"pone.0350029.ref054","doi-asserted-by":"crossref","first-page":"102843","DOI":"10.1016\/j.neucli.2022.102843","article-title":"The cutaneous silent period as a measure of upper motor neuron dysfunction in amyotrophic lateral sclerosis","volume":"53","author":"J Castro","year":"2023","journal-title":"Neurophysiol Clin"},{"key":"pone.0350029.ref055","doi-asserted-by":"crossref","unstructured":"Saidane Y, Melgani F, Jebara SB, Pradat PF, de Marco G. Classification between upper and lower motor neurons in amyotrophic lateral sclerosis patients by electromyographic processing and analysis. 2020 10th International Symposium on Signal, Image, Video and Communications (ISIVC). 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