{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:36:28Z","timestamp":1789050988419,"version":"build-2803163510"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Broad-spectrum antibiotics that are excreted into the intestinal tract disrupt microbiota that provide colonization resistance against healthcare-associated pathogens. Minimizing the duration of treatment is a core element of efforts to reduce the adverse effects of antibiotics, but limited information is available on whether this approach preserves colonization resistance. Here, we used a mouse model to examine the impact of 1, 3, 6, and 10-days of treatment with the broad-spectrum antibiotic piperacillin\/tazobactam on colonization resistance against vancomycin-resistant\n                    <jats:italic>Enterococcus faecium<\/jats:italic>\n                    (VRE) and carbapenemase-producing\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    at 6-, 10-, and 24-days post-treatment; colonization resistance against\n                    <jats:italic>Clostridioides difficile<\/jats:italic>\n                    was tested at 6 days post-treatment. Colonization resistance was not altered at these time points by 1 day of treatment, whereas 3, 6, and 10 days of treatment caused more prolonged microbiota disruption and altered colonization resistance extending to 6, 10, and 24 days, respectively. In contrast, 5 or 10 days of treatment with aztreonam, a narrow-spectrum antibiotic with no activity against anaerobes, did not alter colonization resistance. These findings provide support for efforts to minimize the duration of antibiotic therapy as longer durations of piperacillin\/tazobactam treatment caused greater and more prolonged alteration of colonization resistance. Our results also highlight the potential for short courses of broad-spectrum antibiotics to alter colonization resistance and suggest that selection of antibiotics that cause less disruption of anaerobes may be useful to preserve colonization resistance.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0350031","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T17:31:51Z","timestamp":1780335111000},"page":"e0350031","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Longer durations of piperacillin\/tazobactam treatment cause more prolonged alteration of colonization resistance in mice"],"prefix":"10.1371","volume":"21","author":[{"given":"Bryan S.","family":"Hausman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire E.","family":"Kaple","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Cadnum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3262-5282","authenticated-orcid":true,"given":"Samir","family":"Memic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naseer","family":"Sangwan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0990-4498","authenticated-orcid":true,"given":"Curtis J.","family":"Donskey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"issue":"2","key":"pone.0350031.ref001","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.cmi.2022.08.024","article-title":"Short-course antibiotics for common infections: what do we know and where do we go from here?","volume":"29","author":"RA Lee","year":"2023","journal-title":"Clin Microbiol Infect"},{"issue":"2","key":"pone.0350031.ref002","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.cmi.2022.04.005","article-title":"The Shorter Is Better movement: past, present, future","volume":"29","author":"B Spellberg","year":"2023","journal-title":"Clin Microbiol Infect"},{"key":"pone.0350031.ref003","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1164\/ajrccm.162.2.9909095","article-title":"Short-course empiric antibiotic therapy for patients with pulmonary infiltrates in the intensive care unit. 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