{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T18:05:48Z","timestamp":1779473148557,"version":"3.53.1"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006477","name":"National Taiwan University","doi-asserted-by":"publisher","award":["G049919"],"award-info":[{"award-number":["G049919"]}],"id":[{"id":"10.13039\/501100006477","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    sequence type (ST) 131 is a globally disseminated multidrug-resistant clone that poses a substantial public health threat. This study investigated the prevalence, virulence characteristics, and antimicrobial resistance profiles of ST131 among companion-animal\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates. A total of 400\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates obtained from dogs and cats diagnosed with urinary tract infections at the National Taiwan University Veterinary Hospital between 2011 and 2019 were analyzed. Phylogenetic grouping identified 192 isolates (48.0%) belonging to phylogroup B2, which is strongly associated with ST131. Among these, 26 isolates (13.5%) were confirmed as ST131 by single-nucleotide polymorphism screening and multilocus sequence typing, and 19 isolates were selected for detailed characterization. Virulence gene analysis by multiplex PCR showed that\n                    <jats:italic>fyuA<\/jats:italic>\n                    (100.0%),\n                    <jats:italic>traT<\/jats:italic>\n                    (94.7%),\n                    <jats:italic>iutA<\/jats:italic>\n                    (89.5%), and\n                    <jats:italic>kpsMT<\/jats:italic>\n                    II (84.2%) were the most prevalent genes. Antimicrobial susceptibility testing demonstrated universal susceptibility to meropenem, whereas all isolates were resistant to amoxicillin and ampicillin. Six canine-derived isolates produced extended-spectrum \u03b2-lactamases (ESBLs) or AmpC \u03b2-lactamases. Among ESBL producers,\n                    <jats:italic>bla<\/jats:italic>\n                    gene groups\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M-1<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M-9<\/jats:sub>\n                    , and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM<\/jats:sub>\n                    were detected, whereas\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CMY-178<\/jats:sub>\n                    was the only AmpC gene identified. Conjugation experiments demonstrated transferability for most\n                    <jats:italic>bla<\/jats:italic>\n                    genes, except\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM-102<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM-12<\/jats:sub>\n                    , and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M-55<\/jats:sub>\n                    . Plasmid replicon typing revealed IncF (100%) and IncB\/O (69%) as the predominant plasmid groups. Only two O-antigen types, O25b and O16, were detected among ST131 isolates, which carried\n                    <jats:italic>fimH<\/jats:italic>\n                    variants\n                    <jats:italic>fimH22<\/jats:italic>\n                    ,\n                    <jats:italic>fimH27<\/jats:italic>\n                    ,\n                    <jats:italic>fimH30<\/jats:italic>\n                    , or\n                    <jats:italic>fimH41<\/jats:italic>\n                    subclones. These findings demonstrate the circulation of virulent and multidrug-resistant ST131 strains in companion animals in Taiwan, highlighting their potential relevance within a One Health context.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0350088","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T17:28:07Z","timestamp":1779470887000},"page":"e0350088","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characteristics of Escherichia coli ST131 strains isolated from dogs and cats with urinary tract infections in a teaching hospital in Taiwan"],"prefix":"10.1371","volume":"21","author":[{"given":"Shu-Xian","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3847-7085","authenticated-orcid":true,"given":"Kuang-Sheng","family":"Yeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,5,22]]},"reference":[{"key":"pone.0350088.ref001","first-page":"2669","article-title":"Enterobacteriaceae","volume-title":"Mandell, Douglas, and Bennett\u2019s principles and practice of infectious diseases","author":"GE Nelson","year":"2020"},{"issue":"1","key":"pone.0350088.ref002","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1038\/nrmicro2265","article-title":"Molecular mechanisms of Escherichia coli pathogenicity","volume":"8","author":"MA Croxen","year":"2010","journal-title":"Nat Rev 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