{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T18:07:27Z","timestamp":1780337247281,"version":"3.54.1"},"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Scientific and Technology Project of Inner Mongolia Autonomous Region","award":["2021ZD0006"],"award-info":[{"award-number":["2021ZD0006"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    In order to establish an efficient method for high-throughput detection of\n                    <jats:italic>Yersinia pestis (Y. pestis)<\/jats:italic>\n                    , a quadruple target real-time polymerase chain reaction assay was developed based on the specific target genes of\n                    <jats:italic>Y. pestis<\/jats:italic>\n                    (\n                    <jats:italic>caf1<\/jats:italic>\n                    ,\n                    <jats:italic>pla<\/jats:italic>\n                    ,\n                    <jats:italic>ymt<\/jats:italic>\n                    , and\n                    <jats:italic>ypo-1094<\/jats:italic>\n                    ). Its sensitivity, specificity, and repeatability were evaluated, and clinical serum samples were tested by the established method. The results showed that no cross-reactivity was observed with other bacterial nucleic acids. The optimal linear detection range for\n                    <jats:italic>caf1<\/jats:italic>\n                    ,\n                    <jats:italic>pla<\/jats:italic>\n                    ,\n                    <jats:italic>ymt<\/jats:italic>\n                    , and\n                    <jats:italic>ypo-1094<\/jats:italic>\n                    was 12.09\u2009\u00d7\u200910\n                    <jats:sup>-<\/jats:sup>\n                    \u2076-12.09\u2009\u00d7\u200910\n                    <jats:sup>1<\/jats:sup>\n                    ng\/\u03bcL, and the lower limit of detection was 12.09\u2009\u00d7\u200910\n                    <jats:sup>\u22124<\/jats:sup>\n                    ng\/\u03bcL. Four different DNA concentrations of\n                    <jats:italic>caf1<\/jats:italic>\n                    ,\n                    <jats:italic>pla<\/jats:italic>\n                    ,\n                    <jats:italic>ymt<\/jats:italic>\n                    , and\n                    <jats:italic>ypo-1094<\/jats:italic>\n                    (10\n                    <jats:sup>\u22124<\/jats:sup>\n                    , 10\n                    <jats:sup>\u22123<\/jats:sup>\n                    , 10\n                    <jats:sup>\u22122<\/jats:sup>\n                    , 10\n                    <jats:sup>\u22121<\/jats:sup>\n                    and 10\n                    <jats:sup>1<\/jats:sup>\n                    ng\/\u03bcL) were tested five times, achieving good repeatability. In the clinical sample detection, all the\n                    <jats:italic>Y. pestis<\/jats:italic>\n                    \u2013 positive samples were identified. The established method has potential for clinical use for rapid detection of\n                    <jats:italic>Y. pestis<\/jats:italic>\n                    with high specificity and high sensitivity.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0350660","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T17:28:21Z","timestamp":1780334901000},"page":"e0350660","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment and application of a quadruple target real-time PCR assay for detecting Yersinia pestis"],"prefix":"10.1371","volume":"21","author":[{"given":"Wenfang","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxue","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1980-6085","authenticated-orcid":true,"given":"Zhanli","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"issue":"11","key":"pone.0350660.ref001","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/j.cmi.2016.08.031","article-title":"Molecular history of plague","volume":"22","author":"M Drancourt","year":"2016","journal-title":"Clin Microbiol Infect"},{"issue":"2","key":"pone.0350660.ref002","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.amjmed.2020.08.019","article-title":"History of the plague: an ancient pandemic for the age of COVID-19","volume":"134","author":"KA Glatter","year":"2021","journal-title":"Am J Med"},{"issue":"3","key":"pone.0350660.ref003","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.mib.2012.02.003","article-title":"Yersinia--flea interactions and the evolution of the arthropod-borne transmission route of plague","volume":"15","author":"I Chouikha","year":"2012","journal-title":"Curr Opin Microbiol"},{"issue":"11","key":"pone.0350660.ref004","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0007761","article-title":"Plague in Zimbabwe from 1974 to 2018: a review article","volume":"13","author":"A Munyenyiwa","year":"2019","journal-title":"PLoS Negl Trop Dis"},{"issue":"2","key":"pone.0350660.ref005","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1078\/1438-4639-00259","article-title":"The history of the plague and the research on the causative agent Yersinia pestis","volume":"207","author":"BP Zietz","year":"2004","journal-title":"Int J Hyg Environ Health"},{"key":"pone.0350660.ref006","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1007\/978-94-024-0890-4_12","article-title":"Plague vaccines: status and future","volume":"918","author":"W Sun","year":"2016","journal-title":"Adv Exp Med Biol"},{"issue":"10","key":"pone.0350660.ref007","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.3201\/eid2710.202239","article-title":"Distribution and characteristics of human plague cases and Yersinia pestis isolates from 4 marmota plague foci, China, 1950-2019","volume":"27","author":"Z He","year":"2021","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"pone.0350660.ref008","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1038\/s42003-023-05186-2","article-title":"Genomic diversity of Yersinia pestis from Yunnan Province, China, implies a potential common ancestor as the source of two plague epidemics","volume":"6","author":"J Qin","year":"2023","journal-title":"Commun Biol"},{"issue":"1","key":"pone.0350660.ref009","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.mcp.2010.09.002","article-title":"Detection of Yersinia pestis using real-time PCR in patients with suspected bubonic plague","volume":"25","author":"JM Riehm","year":"2011","journal-title":"Mol Cell Probes"},{"issue":"1","key":"pone.0350660.ref010","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1097\/00019606-199203000-00008","article-title":"The polymerase chain reaction. 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