{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:11:16Z","timestamp":1788909076583,"version":"build-2803163510"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100022230","name":"Deanship of Scientific Research, Princess Nourah Bint Abdulrahman University","doi-asserted-by":"publisher","award":["PNURSP2025R855"],"award-info":[{"award-number":["PNURSP2025R855"]}],"id":[{"id":"10.13039\/501100022230","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Antibiotic resistance in aquaculture settings is an emerging issue that threatens animal and human health. In this work, a multidrug-resistant (MDR)\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    strain (RG3) was isolated from the gill of\n                    <jats:italic>Labeo rohita<\/jats:italic>\n                    obtained from a retail fish market in Punjab, India. Antimicrobial susceptibility testing revealed resistance to various antibiotic groups, with intermediate susceptibility to imipenem. Whole-genome sequencing produced a 3.70 Mb draft genome with 3,814 genes and 39 resistance determinants. Genome analysis identified a chromosomally encoded multidrug resistance phenotype, including \u03b2-lactam resistance and efflux-mediated mechanisms, together with virulence factors associated with iron acquisition, biofilm formation, and host colonization. No plasmids were predicted in the short-read assembly. Genome-based prediction suggested a high likelihood of human pathogenicity and assigned the isolate to sequence type ST8020. This study aimed to characterize an MDR\n                    <jats:italic>E. coli<\/jats:italic>\n                    from an aquaculture source using phenotypic and genomic approaches. The identification of a chromosomally encoded resistome together with virulence-associated traits highlights the potential of aquaculture-derived\n                    <jats:italic>E. coli<\/jats:italic>\n                    to act as reservoirs of antimicrobial resistance within a one health framework.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0351661","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T17:38:00Z","timestamp":1782409080000},"page":"e0351661","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genomic characterization of multidrug-resistant Escherichia coli isolated from gills of Labeo rohita: Insight into resistome, virulence and pathogenicity"],"prefix":"10.1371","volume":"21","author":[{"given":"Amit","family":"Kumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Masood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhouha","family":"Choukaier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhinav","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankit Dilipkumar","family":"Oza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3121-5872","authenticated-orcid":true,"given":"Denekew 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