{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T19:52:00Z","timestamp":1782849120453,"version":"3.54.5"},"reference-count":70,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["2020-04811"],"award-info":[{"award-number":["2020-04811"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, QC, Canada","award":["studentships"],"award-info":[{"award-number":["studentships"]}]},{"name":"Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, QC, Canada","award":["studentships"],"award-info":[{"award-number":["studentships"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Bacterial biofilms are structured communities of bacterial cells enclosed in a self-produced polymeric matrix, which can adhere to biotic or abiotic surfaces. This mode of existence permits these bacteria to endure in adverse conditions, including the presence of antibiotics Bacteria within biofilms are responsible for numerous infections in humans and animals, including bovine mastitis. It is therefore important to develop new therapeutic strategies to control and treat biofilm-associated infections. The results obtained by our group during the study of mixed bacterial biofilm communities showed that four isolates of coagulase-negative staphylococci (CNS; two\n                    <jats:italic>Staphylococcus chromogenes<\/jats:italic>\n                    and two\n                    <jats:italic>Staphylococcus simulans<\/jats:italic>\n                    ) that produce only a small amount of biofilm can significantly reduce biofilm formation in approximatively 80% of pathogenic staphylococci associated with bovine mastitis. Furthermore, supernatants of\n                    <jats:italic>S. chromogenes<\/jats:italic>\n                    reduced secondary intramammary colonization by\n                    <jats:italic>S. aureus<\/jats:italic>\n                    in a murine model of mastitis. However, information regarding the mechanism and the effector molecule(s) involved is lacking. The objective of this study was therefore to investigate and characterize the antibiofilm molecule(s) produced by these four CNS isolates. In this context, we prepared culture supernatants from two isolates of\n                    <jats:italic>S. chromogenes<\/jats:italic>\n                    (C and E) and two isolates of\n                    <jats:italic>S. simulans<\/jats:italic>\n                    (F and H) to evaluate their effect on biofilm production of pathogenic bacterial species involved in bovine mastitis. Using a standard biofilm microtiter plate assay, we demonstrated that the four CNS supernatants not only have a significant impact on biofilms of pathogenic staphylococci (68% of tested isolates) but also on those of other important mastitis pathogens such as\n                    <jats:italic>Streptococcus<\/jats:italic>\n                    spp.,\n                    <jats:italic>Trueperella pyogenes<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    spp. and\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (61.3% of tested isolates)\n                    <jats:italic>.<\/jats:italic>\n                    The isolation and characterization of the antibiofilm molecule(s) contained in the supernatants were then conducted using a filtration process with membranes of different porosities, as well as through physicochemical and enzymatic treatments. We were then able to confirm that antibiofilm activity against staphylococci was present in the\u2009&lt;\u20093kDa fractions of CNS culture supernatants and that this activity was heat-stable and protease-resistant, but sensitive to RNase A, suggesting that the antibiofilm activity might be due, at least in part, to an RNA molecule. Preliminary results showed that the antibiofilm activity was maintained with RNA extracts from fractioned supernatants (&lt;3kDa). In conclusion, these results confirmed that some CNS have an antibiofilm activity, which represents a promising new avenue in the fight against biofilm-associated infections, particularly bovine mastitis.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0351675","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T19:10:51Z","timestamp":1782846651000},"page":"e0351675","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of Antibiofilm Molecules from Bovine Coagulase-Negative Staphylococci"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5073-1141","authenticated-orcid":true,"given":"Coralie","family":"Goetz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibtissem","family":"Doghri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sneha","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Corbeil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Bouveret","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Beaudry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Lalaouna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Mass\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Jacques","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5031-345X","authenticated-orcid":true,"given":"Fran\u00e7ois","family":"Malouin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"pone.0351675.ref001","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.vetmic.2008.09.009","article-title":"Role of coagulase-negative staphylococci in human disease","volume":"134","author":"A Piette","year":"2009","journal-title":"Vet Microbiol"},{"issue":"2","key":"pone.0351675.ref002","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1017\/S1466252310000149","article-title":"Biofilm formation in bacterial pathogens of veterinary importance","volume":"11","author":"M Jacques","year":"2010","journal-title":"Anim Health Res Rev"},{"issue":"12","key":"pone.0351675.ref003","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1586\/14787210.2015.1100533","article-title":"Prevention and treatment of Staphylococcus aureus biofilms","volume":"13","author":"M Bhattacharya","year":"2015","journal-title":"Expert Rev Anti Infect Ther"},{"issue":"8","key":"pone.0351675.ref004","doi-asserted-by":"crossref","first-page":"6454","DOI":"10.3168\/jds.2017-12629","article-title":"Coagulase-negative staphylococci species affect biofilm formation of other coagulase-negative and coagulase-positive staphylococci","volume":"100","author":"C Goetz","year":"2017","journal-title":"J Dairy Sci"},{"issue":"19","key":"pone.0351675.ref005","doi-asserted-by":"crossref","first-page":"6136","DOI":"10.1128\/AEM.01953-14","article-title":"Biofilm formation on biotic and abiotic surfaces in the presence of antimicrobials by Escherichia coli Isolates from cases of bovine mastitis","volume":"80","author":"VO Silva","year":"2014","journal-title":"Appl Environ Microbiol"},{"issue":"6","key":"pone.0351675.ref006","doi-asserted-by":"crossref","first-page":"1877","DOI":"10.3168\/jds.S0022-0302(06)72256-1","article-title":"Invited Review: The role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis","volume":"89","author":"HW Barkema","year":"2006","journal-title":"J Dairy Sci"},{"key":"pone.0351675.ref007","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/978-3-642-21289-5_9","article-title":"Bovine mastitis and biofilms","author":"MB Melchior","year":"2011","journal-title":"Biofilms and veterinary medicine. 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in the dairy industry","volume":"108","author":"C Goetz","year":"2025","journal-title":"J Dairy Sci"},{"issue":"1","key":"pone.0351675.ref023","doi-asserted-by":"crossref","first-page":"104879","DOI":"10.1016\/j.micinf.2021.104879","article-title":"Secondary Staphylococcus aureus intramammary colonization is reduced by non-aureus staphylococci exoproducts","volume":"24","author":"E Brouillette","year":"2022","journal-title":"Microbes Infect"},{"issue":"3","key":"pone.0351675.ref024","doi-asserted-by":"crossref","first-page":"1616","DOI":"10.3168\/jds.2010-3180","article-title":"The National Cohort of Dairy Farms--a data collection platform for mastitis research in Canada","volume":"94","author":"KK Reyher","year":"2011","journal-title":"J Dairy Sci"},{"issue":"15","key":"pone.0351675.ref025","doi-asserted-by":"crossref","DOI":"10.1128\/MRA.00133-19","article-title":"The Mastitis Pathogens Culture Collection","volume":"8","author":"S Dufour","year":"2019","journal-title":"Microbiol 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Microbiol"},{"issue":"3","key":"pone.0351675.ref029","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1099\/mic.0.000878","article-title":"Antibiofilm activity in the culture supernatant of a marine Pseudomonas sp. bacterium","volume":"166","author":"I Doghri","year":"2020","journal-title":"Microbiology (Reading)"},{"issue":"7","key":"pone.0351675.ref030","doi-asserted-by":"crossref","first-page":"2633","DOI":"10.1128\/AAC.48.7.2633-2636.2004","article-title":"Enzymatic detachment of Staphylococcus epidermidis biofilms","volume":"48","author":"JB Kaplan","year":"2004","journal-title":"Antimicrob Agents Chemother"},{"issue":"6","key":"pone.0351675.ref031","doi-asserted-by":"crossref","first-page":"1778","DOI":"10.1007\/s12010-011-9526-2","article-title":"Antibiofilm activity of \u03b1-amylase from Bacillus subtilis S8-18 against biofilm forming human bacterial pathogens","volume":"167","author":"BJ Kalpana","year":"2012","journal-title":"Appl Biochem 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risk, tolerance and control","volume":"64","author":"SL Percival","year":"2015","journal-title":"J Med Microbiol"},{"issue":"2","key":"pone.0351675.ref039","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1038\/nrmicro821","article-title":"Bacterial biofilms: from the natural environment to infectious diseases","volume":"2","author":"L Hall-Stoodley","year":"2004","journal-title":"Nat Rev Microbiol"},{"issue":"1","key":"pone.0351675.ref040","doi-asserted-by":"crossref","first-page":"100","DOI":"10.2174\/1381612820666140905123900","article-title":"Current and future approaches to the prevention and treatment of staphylococcal medical device-related infections","volume":"21","author":"S Hogan","year":"2015","journal-title":"Curr Pharm Des"},{"key":"pone.0351675.ref041","article-title":"Antibiofilm agents: from diagnosis to treatment and prevention","author":"KP Rumbaugh","year":"2014","journal-title":"Heidelberg: 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Gurunathan","year":"2023","journal-title":"Heliyon"},{"key":"pone.0351675.ref045","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.micpath.2013.05.003","article-title":"A new anti-infective strategy to reduce the spreading of antibiotic resistance by the action on adhesion-mediated virulence factors in Staphylococcus aureus","volume":"63","author":"R Papa","year":"2013","journal-title":"Microb Pathog"},{"issue":"3","key":"pone.0351675.ref046","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1128\/br.40.3.722-756.1976","article-title":"Bacteriocins of gram-positive bacteria","volume":"40","author":"JR Tagg","year":"1976","journal-title":"Bacteriol Rev"},{"issue":"1","key":"pone.0351675.ref047","first-page":"13","article-title":"Bact\u00e9riophages et phagoth\u00e9rapie: utilisation de virus naturels pour traiter les infections bact\u00e9riennes","volume":"28","author":"F Ravat","year":"2015","journal-title":"Ann Burns Fire 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Microbiology"},{"issue":"1","key":"pone.0351675.ref051","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/1462-2920.14834","article-title":"Unravelling interspecies interactions across heterogeneities in complex biofilm communities","volume":"22","author":"HL R\u00f8der","year":"2020","journal-title":"Environ Microbiol"},{"key":"pone.0351675.ref052","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.vetmic.2004.10.014","article-title":"Production of bacteriocins by coagulase-negative staphylococci involved in bovine mastitis","volume":"106","author":"J dos Santos Nascimento","year":"2005","journal-title":"Vet Microbiol"},{"key":"pone.0351675.ref053","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.vetmic.2010.04.032","article-title":"Nukacin 3299, a lantibiotic produced by Staphylococcus simulans 3299 identical to nukacin ISK-1","volume":"146","author":"H Ceotto","year":"2010","journal-title":"Vet 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