{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T18:28:14Z","timestamp":1782930494054,"version":"3.54.5"},"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FIOTEC\/FIOCRUZ","award":["project DIREX-003-FIO-24"],"award-info":[{"award-number":["project DIREX-003-FIO-24"]}]},{"name":"FIOTEC\/FIOCRUZ","award":["project DIREX-003-FIO-24"],"award-info":[{"award-number":["project DIREX-003-FIO-24"]}]},{"name":"FIOTEC\/FIOCRUZ","award":["project DIREX-003-FIO-24"],"award-info":[{"award-number":["project DIREX-003-FIO-24"]}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Instituto Evandro Chagas"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Human astrovirus (HAstV) is a viral agent responsible for acute gastroenteritis (AGE), primarily affecting children and the elderly worldwide. Belonging to the\n                    <jats:italic>Astroviridae<\/jats:italic>\n                    family, HAstV is classified into eight classical serotypes (HAstV 1\u20138) and two other divergent non-classical clades: Melbourne (MLB 1\u20133) and Virginia (VA 1\u20136), which have been associated with gastroenteritis, central nervous system complications, and acute respiratory disease. This study aimed to investigate the frequency of classical and non-classical HAstV in fecal specimens collected from children up to 14 years of age in northern Brazil, within the Amazon region, between 2013 and 2022. A total of 560 samples, all previously tested negative for other gastroenteric viruses such as rotavirus and norovirus, were analyzed using reverse transcription followed by quantitative polymerase chain reaction (RT-qPCR) and conventional RT-PCR. For classical HAstV, 10.7% (60\/560) of the samples were positive by RT-qPCR and 2.0% (11\/560) to 3.0% (17\/560) by conventional RT-PCR using different primers set. Non-classical HAstV was detected in 0.2% (1\/560) of the samples. Diarrhea was present in 91.7% of positive cases, vomiting in 71.7%, and fever in 53.3%. The most affected age group was children aged &gt;5\u201310 years (25.0%), with no significant association between infection rate and sex. A higher number of infections occurred during the Amazon winter (11.6%), with Roraima identified as the federative unit with the highest number of cases. Fifteen samples (88.2%, 15\/17) were sequenced and identified as classical HAstV, with the following genotypes detected: HAstV-1 (60.0%, 9\/15), HAstV-3 (20.0%, 3\/15), and HAstV-4 (20.0%, 3\/15). Non-classical HAstV sequencing was performed on 12 (93.2%, 12\/13) positive specimens, characterized as HAstV-1 (50%, 6\/12), HAstV-3 (25%, 3\/12), HAstV-4 (16.7%, 2\/12), and HAstV-MLB-3 (8.3%, 1\/12). A probable recombinant strain was identified, classified as HAstV-4 based on the ORF2 region and HAstV-1 based on the ORF1b region. This study provides updated epidemiological data on HAstV in the Brazilian Amazon and highlights the genetic diversity of both classical and non-classical genotypes. Notably, it reports the first detection \u2013 and the second complete genome repository \u2013 of the rare MLB-3 genotype in the Americas.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0352094","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T17:40:33Z","timestamp":1782927633000},"page":"e0352094","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Astrovirus in the Brazilian Amazon: First detection of non-classical astroviruses (MLB-3) in the Americas"],"prefix":"10.1371","volume":"21","author":[{"given":"Dan Santos","family":"Alves","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dielle Monteiro","family":"Teixeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edivaldo Costa","family":"Sousa Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1153-5508","authenticated-orcid":true,"given":"Danielle Rodrigues de","family":"Deus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia dos Santos","family":"Lobo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia de F\u00e1tima dos Santos","family":"Guerra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana Damascena da","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo Reis","family":"Resque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana Silva","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvone Benchimol","family":"Gabbay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6491-6590","authenticated-orcid":true,"given":"Jones Anderson Monteiro","family":"Siqueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,7,1]]},"reference":[{"issue":"10429","key":"pone.0352094.ref001","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1016\/S0140-6736(23)02037-8","article-title":"Viral gastroenteritis","volume":"403","author":"TG Flynn","year":"2024","journal-title":"Lancet"},{"issue":"2","key":"pone.0352094.ref002","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.gtc.2021.02.003","article-title":"Viral Acute Gastroenteritis in Special Populations","volume":"50","author":"JL Meier","year":"2021","journal-title":"Gastroenterol Clin North Am"},{"issue":"10142","key":"pone.0352094.ref003","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0140-6736(18)31128-0","article-title":"Viral gastroenteritis","volume":"392","author":"K B\u00e1nyai","year":"2018","journal-title":"Lancet"},{"key":"pone.0352094.ref004","article-title":"Viral Gastroenteritis (Nursing)","volume-title":"StatPearls","author":"ND Stuempfig","year":"2023"},{"issue":"1","key":"pone.0352094.ref005","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1159\/000540253","article-title":"Molecular Characterization of Enteric Viruses Causing Acute Gastroenteritis among Children under 5 Years Old in Distrito Central, Honduras","volume":"67","author":"J Ortiz-Quintero","year":"2024","journal-title":"Intervirology"},{"key":"pone.0352094.ref006","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.1016\/B978-0-12-818619-0.00001-0","article-title":"Chapter 115 - Gastroenteritis viruses other than rotaviruses.","volume-title":"Molecular Medical Microbiology (Third Edition)","author":"X. 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