{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T17:50:29Z","timestamp":1782237029710,"version":"3.54.5"},"reference-count":74,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Center of Excellence in Materials Science and Technology, Faculty of Science, Chiang Mai University"},{"name":"Chiang Mai University and Thailand Science Research and Innovation (TSRI): Fundamental Fund 2024","award":["FF043\/2567"],"award-info":[{"award-number":["FF043\/2567"]}]},{"name":"the Science Achievement Scholarship of Thailand"},{"name":"the Development and Promotion of Science and Technology Talents Project (DPST) Scholarship"},{"name":"Chiang Mai University Presidential Scholarship"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Breast cancer is the most frequently diagnosed cancer and remains the leading cause of death among females. Despite the rapid advancement in cancer therapy, the development of more effective therapeutic strategies and anticancer agents with a new mode of action remains a critical challenge. Combination of therapeutic agents is an attractive approach to enhance drug efficacy. Nisin, a cationic antimicrobial peptide, has been reported for its cytotoxicity against some cancer cell lines via several mechanisms, particularly membrane disruption. Nisin can bind preferentially to the negatively charged phospholipids, causing pore formation in cell membranes. In this study, we demonstrated that nisin exhibited membrane permeabilization preferentially with anionic lipids using liposomal leakage assay. Moreover, anticancer activity of nisin and doxorubicin (DOX) was investigated against two breast cancer cell lines, MCF-7 and MDA-MB-231, using MTT assay. Nisin demonstrated cytotoxic effect against breast cancer MCF-7 and MDA-MB-231, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 5\u20138 \u00b5M, while exhibiting lower cytotoxicity toward normal cells. Based on Bliss independence analysis, the synergistic effect between nisin and DOX was markedly observed in MCF-7 when treated with 10 \u03bcM nisin and 1 \u03bcM DOX for 24\u201348\u2009h treatment. Furthermore, as demonstrated by fluorescent-based high-content analysis, nisin clearly caused cell membrane permeability and promoted DOX-induced DNA damage in both cell lines. Flow cytometry with Annexin-V staining revealed that co-treatment of nisin and DOX significantly enhanced apoptosis, as compared to individual treatments, particularly in MCF-7 cells, suggesting a key mechanism of action for anticancer activity. Cationic nisin can interact with biological membrane and modulate membrane permeability and fluidity, consequently facilitating DOX entry, inducing apoptosis and DNA damage. Overall, this study demonstrates that the combination of nisin and DOX could offer a new therapeutic approach for breast cancer treatment with a reduced chemotherapeutic drug dosage.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0352312","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T17:27:02Z","timestamp":1782235622000},"page":"e0352312","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synergistic anticancer activity of antimicrobial peptide nisin and doxorubicin against breast cancer cells via modulation of membrane permeability"],"prefix":"10.1371","volume":"21","author":[{"given":"Chanita","family":"Phetdee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suwatjanee","family":"Naephrai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malinee","family":"Pradain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1387-9677","authenticated-orcid":true,"given":"Yanisa","family":"Panporm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ubaid","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6376-7116","authenticated-orcid":true,"given":"Panchika","family":"Prangkio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"issue":"1","key":"pone.0352312.ref001","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1038\/s41597-023-02253-5","article-title":"Global 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Wang","year":"2024","journal-title":"Biomolecules"},{"key":"pone.0352312.ref033","doi-asserted-by":"crossref","first-page":"999883","DOI":"10.3389\/fphys.2022.999883","article-title":"Alteration of cholesterol distribution at the plasma membrane of cancer cells: from evidence to pathophysiological implication and promising therapy strategy","volume":"13","author":"M Maja","year":"2022","journal-title":"Front Physiol"},{"key":"pone.0352312.ref034","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.1021\/acs.jcim.5c02731","article-title":"Current status of molecular dynamics simulations of membrane permeabilization by antimicrobial peptides and pore-forming proteins: a review","volume":"66","author":"S Cresca","year":"2026","journal-title":"J Chem Inf Model"},{"key":"pone.0352312.ref035","doi-asserted-by":"crossref","first-page":"102269","DOI":"10.1101\/pdb.prot102269","article-title":"Bradford assay for determining protein concentration","volume":"2020","author":"CL Kielkopf","year":"2020","journal-title":"Cold Spring Harb Protoc"},{"key":"pone.0352312.ref036","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1515\/ntrev-2016-0081","article-title":"Proteoliposomes \u2013 a system to study membrane proteins under buffer gradients by cryo-EM","volume":"6","author":"K Sejwal","year":"2017","journal-title":"Nanotechnol Rev"},{"issue":"12","key":"pone.0352312.ref037","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0085467","article-title":"The effect of hydrophilic ionic liquids 1-ethyl-3-methylimidazolium lactate and choline lactate on lipid vesicle fusion","volume":"8","author":"EH Hayakawa","year":"2013","journal-title":"PLoS One"},{"key":"pone.0352312.ref038","doi-asserted-by":"crossref","first-page":"2206","DOI":"10.1021\/acs.biochem.2c00495","article-title":"Ganglioside-enriched phospholipid vesicles induce cooperative A\u03b2 oligomerization and membrane disruption","volume":"61","author":"J Saha","year":"2022","journal-title":"Biochemistry"},{"issue":"3","key":"pone.0352312.ref039","doi-asserted-by":"crossref","first-page":"236","DOI":"10.2174\/0929866525666171201120456","article-title":"Contributions of the Hydrophobic Helix 2 of the Bordetella pertussis CyaA-hemolysin to Membrane Permeabilization","volume":"25","author":"P Prangkio","year":"2018","journal-title":"Protein Pept Lett"},{"key":"pone.0352312.ref040","doi-asserted-by":"crossref","DOI":"10.21769\/BioProtoc.2433","article-title":"Liposome disruption assay to examine lytic properties of biomolecules","volume":"7","author":"JR Jimah","year":"2017","journal-title":"Bio-protocol"},{"issue":"5","key":"pone.0352312.ref041","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1007\/BF02531316","article-title":"Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots","volume":"5","author":"G Rouser","year":"1970","journal-title":"Lipids"},{"issue":"2","key":"pone.0352312.ref042","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1080\/19466315.2018.1437071","article-title":"Evaluation of drug combination effect using a Bliss independence dose-response surface model","volume":"10","author":"Q Liu","year":"2018","journal-title":"Stat Biopharm Res"},{"key":"pone.0352312.ref043","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0005-2736(99)00208-4","article-title":"The lantibiotic nisin, a special case or not?","volume":"1462","author":"E Breukink","year":"1999","journal-title":"Biochim Biophys Acta"},{"issue":"11","key":"pone.0352312.ref044","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.3390\/membranes12111166","article-title":"Discovery of the potentiator of the pore-forming ability of lantibiotic nisin: perspectives for anticancer therapy","volume":"12","author":"DN Chernyshova","year":"2022","journal-title":"Membranes 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