{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T17:50:14Z","timestamp":1782409814980,"version":"3.54.5"},"reference-count":131,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Gazi University Scientific Research Projects Coordination Unit","award":["TDK-2022-7895"],"award-info":[{"award-number":["TDK-2022-7895"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Apolipoprotein B (Apo B), which is involved in the transport of cholesterol, is thought to be associated with neurodegenerative diseases such as Parkinson\u2019s disease in addition to atherosclerosis and cardiovascular diseases. We aimed to investigate the possible correlation between cholesterol intake, Apo B and parkin RING domain-containing E3 ubiquitin protein ligase (PARKIN), phosphatase and tensin homologue (PTEN)-induced kinase 1 (PINK1) and \u03b1-synuclein (SNCA), which have an important role in Parkinson\u2019s disease. Throughout the 12-week experiment, female and male guinea pigs in control group were fed a standard chow diet, while those in experimental group were fed a high-fat diet containing cholesterol. When histochemical findings were analysed at the end of our study, neuronal degeneration in the midbrain and brain cortex sections of the group of male guinea pigs fed a high-fat diet containing cholesterol was more pronounced compared to the other groups. In addition, significant differences were observed between the groups in terms of PARKIN expression levels (p\u2009=\u20090.030) in the brain tissues and the immunolabeling densities of PINK1 (p\u2009=\u20090.027), phospho(ser228)-PINK1 (p\u2009=\u20090.031), phospho(ser129)-SNCA (p\u2009&lt;\u20090.000), and tyrosine hydroxylase (TH) (p\u2009=\u20090.033), particularly in the midbrain sections. Significant strong positive correlations (+0.5\u2009&lt;\u2009r&lt;+1.0, p\u2009&lt;\u20090.05) were observed in midbrain sections between phospho(Ser228)-PINK1 and TH immunolabeling and cholesterol (CHOL) levels, between phospho(Ser228)-PINK1 immunolabeling and low-density lipoprotein (LDL) levels, and between SNCA, phospho(Ser228)-PINK1, phospho(Ser129)-SNCA, and TH immunolabeling and high-density lipoprotein (HDL) levels. Our study demonstrated that a high-fat diet containing cholesterol was associated with significant changes in PARKIN gene expression and significant alterations in PINK1 protein levels in male guinea pigs in the experimental group.<\/jats:p>","DOI":"10.1371\/journal.pone.0352642","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T17:38:38Z","timestamp":1782409118000},"page":"e0352642","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigation of correlation between cholesterol intake, apolipoprotein B and Parkinson\u2019s disease related genes in guinea pigs feeding a high-fat diet containing cholesterol"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5650-9664","authenticated-orcid":true,"given":"Pinar","family":"Kacamak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cigdem","family":"Elmas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatice Ayse","family":"Tokcaer Bora","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seniha Selcen","family":"Babaoglu Aydas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,6,25]]},"reference":[{"issue":"4","key":"pone.0352642.ref001","first-page":"182","article-title":"Parkinson Hastal\u0131\u011f\u0131 ve Parkinsonizm","volume":"13","author":"D Kaya","year":"2022","journal-title":"JTFP"},{"key":"pone.0352642.ref002","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.bbr.2019.01.041","article-title":"Sex differences in the progressive model of parkinsonism induced by reserpine in rats","volume":"363","author":"JMM Bispo","year":"2019","journal-title":"Behav Brain Res"},{"issue":"9","key":"pone.0352642.ref003","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1212\/WNL.55.9.1358","article-title":"Parkinson\u2019s disease and parkinsonism in a longitudinal study: two-fold higher incidence in men","volume":"55","author":"M Baldereschi","year":"2000","journal-title":"Neurology"},{"issue":"3","key":"pone.0352642.ref004","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1586\/14737175.6.3.407","article-title":"Gender disparities in Parkinson\u2019s disease","volume":"6","author":"LM Shulman","year":"2006","journal-title":"Expert Rev Neurother"},{"issue":"8","key":"pone.0352642.ref005","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1007\/s00415-016-8384-9","article-title":"The relevance of gender in Parkinson\u2019s disease: a review","volume":"264","author":"M Picillo","year":"2017","journal-title":"J Neurol"},{"issue":"8","key":"pone.0352642.ref006","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1002\/gps.4414","article-title":"Estimating the prevalence of Parkinson\u2019s disease (PD) and proportions of patients with associated dementia and depression among the older adults based on secondary claims data","volume":"31","author":"O Riedel","year":"2016","journal-title":"Int J Geriatr Psychiatry"},{"key":"pone.0352642.ref007","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.expneurol.2014.03.010","article-title":"Sex differences in Parkinson\u2019s disease and other movement disorders","volume":"259","author":"KM Smith","year":"2014","journal-title":"Exp Neurol"},{"issue":"7252","key":"pone.0352642.ref008","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1136\/bmj.321.7252.21","article-title":"Cross sectional prevalence survey of idiopathic Parkinson\u2019s disease and Parkinsonism in London","volume":"321","author":"A Schrag","year":"2000","journal-title":"BMJ"},{"issue":"3","key":"pone.0352642.ref009","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1212\/01.wnl.0000171344.87802.94","article-title":"Effect of reproductive factors and postmenopausal hormone use on the risk of Parkinson disease","volume":"65","author":"RA Popat","year":"2005","journal-title":"Neurology"},{"issue":"5","key":"pone.0352642.ref010","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1007\/s12272-019-01131-2","article-title":"Peroxisomal dysfunction in neurodegenerative diseases","volume":"42","author":"DS Jo","year":"2019","journal-title":"Arch Pharm Res"},{"issue":"1","key":"pone.0352642.ref011","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3233\/JPD-140491","article-title":"Role of \u03b1-synuclein in inducing innate and adaptive immunity in Parkinson disease","volume":"5","author":"HE Allen Reish","year":"2015","journal-title":"J Parkinsons Dis"},{"issue":"13","key":"pone.0352642.ref012","first-page":"1","article-title":"Etiopathogenesis of parkinson\u2019s disease","volume":"7","author":"E Akbay\u0131r","year":"2017","journal-title":"Experimed"},{"key":"pone.0352642.ref013","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.freeradbiomed.2013.01.018","article-title":"Parkinson disease: from pathology to molecular disease mechanisms","volume":"62","author":"DT Dexter","year":"2013","journal-title":"Free Radic Biol Med"},{"issue":"3","key":"pone.0352642.ref014","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1007\/s00401-012-1013-5","article-title":"The genetics and neuropathology of Parkinson\u2019s disease","volume":"124","author":"H Houlden","year":"2012","journal-title":"Acta Neuropathol"},{"issue":"6","key":"pone.0352642.ref015","article-title":"A two-stage meta-analysis identifies several new loci for Parkinson\u2019s disease","volume":"7","author":"International Parkinson\u2019s Disease Genomics Consortium (IPDGC), Wellcome Trust Case Control Consortium 2 (WTCCC2)","year":"2011","journal-title":"PLoS Genet"},{"issue":"9766","key":"pone.0352642.ref016","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/S0140-6736(10)62345-8","article-title":"Imputation of sequence variants for identification of genetic risks for Parkinson\u2019s disease: a meta-analysis of genome-wide association studies","volume":"377","author":"International Parkinson Disease Genomics Consortium","year":"2011","journal-title":"Lancet"},{"issue":"12","key":"pone.0352642.ref017","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1515\/cclm-2013-0358","article-title":"Recent advances in physiological lipoprotein metabolism","volume":"52","author":"I Ramasamy","year":"2014","journal-title":"Clin Chem Lab Med"},{"key":"pone.0352642.ref018","author":"KR Feingold","year":"2021"},{"key":"pone.0352642.ref019","doi-asserted-by":"crossref","DOI":"10.1093\/jn\/128.2.439S","article-title":"Discovery of the lipoproteins, their role in fat transport and their significance as risk factors","volume":"128","author":"RE Olson","year":"1998","journal-title":"J Nutr"},{"issue":"2","key":"pone.0352642.ref020","doi-asserted-by":"crossref","first-page":"295","DOI":"10.3390\/cells11020295","article-title":"Long-Term High-Fat Diet Consumption Depletes Glial Cells and Tyrosine Hydroxylase-Containing Neurons in the Brain of Middle-Aged Rats","volume":"11","author":"M-C Chou","year":"2022","journal-title":"Cells"},{"issue":"1","key":"pone.0352642.ref021","doi-asserted-by":"crossref","first-page":"11691","DOI":"10.1038\/s41598-018-29639-5","article-title":"Impaired HDL cholesterol efflux capacity in patients with non-alcoholic fatty liver disease is associated with subclinical atherosclerosis","volume":"8","author":"R Fadaei","year":"2018","journal-title":"Sci Rep"},{"issue":"5","key":"pone.0352642.ref022","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1097\/HJH.0b013e328337a9e3","article-title":"Impact of different definitions of the metabolic syndrome on the prevalence of organ damage, cardiometabolic risk and cardiovascular events","volume":"28","author":"G Mancia","year":"2010","journal-title":"J Hypertens"},{"issue":"11","key":"pone.0352642.ref023","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1016\/j.jdiacomp.2018.08.001","article-title":"Body fat, metabolic syndrome and hyperglycemia in South Asians","volume":"32","author":"A Misra","year":"2018","journal-title":"J Diabetes Complications"},{"key":"pone.0352642.ref024","doi-asserted-by":"crossref","first-page":"729194","DOI":"10.1155\/2014\/729194","article-title":"Metabolic syndrome: an important risk factor for Parkinson\u2019s disease","volume":"2014","author":"P Zhang","year":"2014","journal-title":"Oxid Med Cell Longev"},{"key":"pone.0352642.ref025","doi-asserted-by":"crossref","first-page":"474296","DOI":"10.1155\/2014\/474296","article-title":"Ketogenic diet in neuromuscular and neurodegenerative diseases","volume":"2014","author":"A Paoli","year":"2014","journal-title":"Biomed Res Int"},{"issue":"2","key":"pone.0352642.ref026","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.expneurol.2012.01.024","article-title":"iTRAQ and multiple reaction monitoring as proteomic tools for biomarker search in cerebrospinal fluid of patients with Parkinson\u2019s disease dementia","volume":"234","author":"S Lehnert","year":"2012","journal-title":"Exp Neurol"},{"issue":"3","key":"pone.0352642.ref027","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1007\/s11011-020-00665-5","article-title":"Lipid metabolic dysregulation is involved in Parkinson\u2019s disease dementia","volume":"36","author":"M-X Dong","year":"2021","journal-title":"Metab Brain Dis"},{"issue":"9","key":"pone.0352642.ref028","article-title":"Reduced serum levels of triglyceride, very low density lipoprotein cholesterol and apolipoprotein B in Parkinson\u2019s disease patients","volume":"8","author":"Q Wei","year":"2013","journal-title":"PLoS One"},{"key":"pone.0352642.ref029","doi-asserted-by":"crossref","first-page":"80","DOI":"10.3389\/fnmol.2020.00080","article-title":"Integrated Metabolomics and Proteomics Analysis Reveals Plasma Lipid Metabolic Disturbance in Patients With Parkinson\u2019s Disease","volume":"13","author":"L Hu","year":"2020","journal-title":"Front Mol Neurosci"},{"issue":"6","key":"pone.0352642.ref030","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1161\/CIRCRESAHA.119.314929","article-title":"Lipids, Apolipoproteins, and the Risk of Parkinson Disease: A Prospective Cohort Study and a Mendelian Randomization Analysis","volume":"125","author":"F Fang","year":"2019","journal-title":"Circ Res"},{"key":"pone.0352642.ref031","doi-asserted-by":"crossref","first-page":"105112","DOI":"10.1016\/j.chemphyslip.2021.105112","article-title":"The disturbance of lipid metabolism is correlated with neuropsychiatric symptoms in patients with Parkinson\u2019s disease","volume":"239","author":"M-X Dong","year":"2021","journal-title":"Chem Phys Lipids"},{"key":"pone.0352642.ref032","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.parkreldis.2021.02.007","article-title":"Association of lipid levels with motor and cognitive function and decline in advanced Parkinson\u2019s disease in the Mark-PD study","volume":"85","author":"C-U Choe","year":"2021","journal-title":"Parkinsonism Relat Disord"},{"key":"pone.0352642.ref033","doi-asserted-by":"crossref","first-page":"380","DOI":"10.3389\/fncel.2018.00380","article-title":"Cerebrovascular Pathology in Hypertriglyceridemic APOB-100 Transgenic Mice","volume":"12","author":"Z Hoyk","year":"2018","journal-title":"Front Cell Neurosci"},{"issue":"1","key":"pone.0352642.ref034","doi-asserted-by":"crossref","first-page":"51","DOI":"10.18097\/pbmc20216701051","article-title":"Changes in the mitochondrial subproteome of mouse brain Rpn13-binding proteins induced by the neurotoxin MPTP and the neuroprotector isatin","volume":"67","author":"OA Buneeva","year":"2021","journal-title":"Biomed Khim"},{"issue":"5","key":"pone.0352642.ref035","doi-asserted-by":"crossref","first-page":"2577","DOI":"10.3390\/ijms23052577","article-title":"Fatty Acids: An Insight into the Pathogenesis of Neurodegenerative Diseases and Therapeutic Potential","volume":"23","author":"DJ Vesga-Jim\u00e9nez","year":"2022","journal-title":"Int J Mol Sci"},{"issue":"2","key":"pone.0352642.ref036","first-page":"127","article-title":"Neuroprotection of sex steroids","volume":"35","author":"M Liu","year":"2010","journal-title":"Minerva Endocrinol"},{"issue":"5","key":"pone.0352642.ref037","doi-asserted-by":"crossref","first-page":"554","DOI":"10.5607\/en.2019.28.5.554","article-title":"Cholesterol Metabolism in the Brain and Its Association with Parkinson\u2019s Disease","volume":"28","author":"U Jin","year":"2019","journal-title":"Exp Neurobiol"},{"issue":"7","key":"pone.0352642.ref038","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1161\/ATVBAHA.116.307023","article-title":"Central Nervous System Lipoproteins: ApoE and Regulation of Cholesterol Metabolism","volume":"36","author":"RW Mahley","year":"2016","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"10","key":"pone.0352642.ref039","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.15252\/embr.201439225","article-title":"Cholesterol in brain disease: sometimes determinant and frequently implicated","volume":"15","author":"MG Mart\u00edn","year":"2014","journal-title":"EMBO Rep"},{"issue":"5262","key":"pone.0352642.ref040","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1126\/science.272.5262.685","article-title":"Mouse models of atherosclerosis","volume":"272","author":"JL Breslow","year":"1996","journal-title":"Science"},{"issue":"13","key":"pone.0352642.ref041","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.2174\/1381612826666200218101818","article-title":"Cerebrovascular Changes and Neurodegeneration Related to Hyperlipidemia: Characteristics of the Human ApoB-100 Transgenic Mice","volume":"26","author":"ME T\u00f3th","year":"2020","journal-title":"Curr Pharm Des"},{"key":"pone.0352642.ref042","author":"S Frank","year":"2012","journal-title":"Lipoproteins-role in health and diseases"},{"issue":"1","key":"pone.0352642.ref043","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1093\/jn\/131.1.10","article-title":"Guinea pigs as models for cholesterol and lipoprotein metabolism","volume":"131","author":"ML Fernandez","year":"2001","journal-title":"J Nutr"},{"issue":"9","key":"pone.0352642.ref044","doi-asserted-by":"crossref","first-page":"1766","DOI":"10.1093\/gerona\/glac073","article-title":"Nontransgenic Guinea Pig Strains Exhibit Hallmarks of Human Brain Aging and Alzheimer\u2019s Disease","volume":"77","author":"D Wahl","year":"2022","journal-title":"J Gerontol A Biol Sci Med Sci"},{"issue":"6","key":"pone.0352642.ref045","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1016\/j.neurobiolaging.2013.11.020","article-title":"Aging, cortical injury and Alzheimer\u2019s disease-like pathology in the guinea pig brain","volume":"35","author":"K Bates","year":"2014","journal-title":"Neurobiol Aging"},{"issue":"6","key":"pone.0352642.ref046","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0066235","article-title":"The Guinea Pig as a Model for Sporadic Alzheimer\u2019s Disease (AD): The Impact of Cholesterol Intake on Expression of AD-Related Genes","volume":"8","author":"MJ Sharman","year":"2013","journal-title":"PLoS One"},{"key":"pone.0352642.ref047","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.nut.2017.07.005","article-title":"Effects of betaine supplementation on nitric oxide metabolism, atherosclerotic parameters, and fatty liver in guinea pigs fed a high cholesterol plus methionine diet","volume":"45","author":"Z K\u00fcsk\u00fc-Kiraz","year":"2018","journal-title":"Nutrition"},{"issue":"5","key":"pone.0352642.ref048","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1097\/01.mao.0000226286.19295.34","article-title":"Dyslipidemia and auditory function","volume":"27","author":"MB Evans","year":"2006","journal-title":"Otol Neurotol"},{"issue":"2","key":"pone.0352642.ref049","doi-asserted-by":"crossref","first-page":"823","DOI":"10.3892\/etm.2021.10255","article-title":"Changes in the interstitial cells of Cajal in the gallbladder of guinea pigs fed a lithogenic diet","volume":"22","author":"L Huang","year":"2021","journal-title":"Exp Ther Med"},{"issue":"1","key":"pone.0352642.ref050","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1186\/s12944-019-1159-4","article-title":"Pioglitazone prevents cholesterol gallstone formation through the regulation of cholesterol homeostasis in guinea pigs with a lithogenic diet","volume":"18","author":"T Han","year":"2019","journal-title":"Lipids Health Dis"},{"key":"pone.0352642.ref051","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.trsl.2015.10.001","article-title":"Diet-induced dyslipidemia leads to nonalcoholic fatty liver disease and oxidative stress in guinea pigs","volume":"168","author":"P Tveden-Nyborg","year":"2016","journal-title":"Transl Res"},{"issue":"7","key":"pone.0352642.ref052","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1093\/jn\/121.7.934","article-title":"Regulation of cholesterol and lipoprotein metabolism in guinea pigs mediated by dietary fat quality and quantity","volume":"121","author":"ML Fernandez","year":"1991","journal-title":"J Nutr"},{"issue":"10","key":"pone.0352642.ref053","doi-asserted-by":"crossref","first-page":"2019","DOI":"10.1093\/jn\/122.10.2019","article-title":"Dietary fat type and cholesterol quantity interact to affect cholesterol metabolism in guinea pigs","volume":"122","author":"EC Lin","year":"1992","journal-title":"J Nutr"},{"issue":"11","key":"pone.0352642.ref054","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1007\/s11745-001-0834-1","article-title":"Soluble fiber and soybean protein reduce atherosclerotic lesions in guinea pigs. Sex and hormonal status determine lesion extension","volume":"36","author":"E Cos","year":"2001","journal-title":"Lipids"},{"issue":"7","key":"pone.0352642.ref055","doi-asserted-by":"crossref","first-page":"2268","DOI":"10.1093\/jn\/133.7.2268","article-title":"Grape polyphenols decrease plasma triglycerides and cholesterol accumulation in the aorta of ovariectomized guinea pigs","volume":"133","author":"TL Zern","year":"2003","journal-title":"J Nutr"},{"issue":"1","key":"pone.0352642.ref056","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/S0041-008X(03)00098-X","article-title":"Oxidative DNA damage in vitamin C-supplemented guinea pigs after intratracheal instillation of diesel exhaust particles","volume":"189","author":"P M\u00f8ller","year":"2003","journal-title":"Toxicol Appl Pharmacol"},{"issue":"1","key":"pone.0352642.ref057","first-page":"1","article-title":"The effects of diet on anatomy, physiology and health in the guinea pig","volume":"1","author":"A Witkowska","year":"2017","journal-title":"J Anim Health Behav Sci"},{"issue":"5","key":"pone.0352642.ref058","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1111\/cns.13805","article-title":"Neuroprotective effects of curcumin on the cerebellum in a rotenone-induced Parkinson\u2019s Disease Model","volume":"28","author":"H Fikry","year":"2022","journal-title":"CNS Neurosci Ther"},{"issue":"04","key":"pone.0352642.ref059","doi-asserted-by":"crossref","first-page":"216","DOI":"10.4236\/wjns.2020.104021","article-title":"Studies on Parkinson\u2019s-Disease-Linked Genes, Brain Urea Levels and Histopathology in Rotenone Induced Parkinson\u2019s Disease Rat Model","volume":"10","author":"S Kavuri","year":"2020","journal-title":"WJNS"},{"issue":"2","key":"pone.0352642.ref060","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1615\/JEnvironPatholToxicolOncol.2018025666","article-title":"Melatonin Improves Behavioral and Biochemical Outcomes in a Rotenone-Induced Rat Model of Parkinson\u2019s Disease","volume":"37","author":"MZ Rasheed","year":"2018","journal-title":"J Environ Pathol Toxicol Oncol"},{"issue":"1","key":"pone.0352642.ref061","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s40035-023-00368-8","article-title":"Animal models of Parkinson\u2019s disease: bridging the gap between disease hallmarks and research questions","volume":"12","author":"A Dovonou","year":"2023","journal-title":"Transl Neurodegener"},{"issue":"1","key":"pone.0352642.ref062","doi-asserted-by":"crossref","first-page":"99","DOI":"10.4149\/gpb_2016031","article-title":"Cerebellar neurochemical and histopathological changes in rat model of Parkinson\u2019s disease induced by intrastriatal injection of rotenone","volume":"36","author":"YA Khadrawy","year":"2017","journal-title":"Gen Physiol Biophys"},{"issue":"4","key":"pone.0352642.ref063","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"issue":"1","key":"pone.0352642.ref064","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1186\/s13075-019-1925-8","article-title":"Calorie restriction with regular chow, but not a high-fat diet, delays onset of spontaneous osteoarthritis in the Hartley guinea pig model","volume":"21","author":"LB Radakovich","year":"2019","journal-title":"Arthritis Res Ther"},{"issue":"2","key":"pone.0352642.ref065","first-page":"151","article-title":"A model of type 2 diabetes in the guinea pig using sequential diet-induced glucose intolerance and streptozotocin treatment","volume":"10","author":"BK Podell","year":"2017","journal-title":"Dis Model Mech"},{"issue":"12","key":"pone.0352642.ref066","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/S0955-2863(02)00219-X","article-title":"Exercise improves plasma lipid profiles and modifies lipoprotein composition in guinea pigs","volume":"13","author":"WY Ensign","year":"2002","journal-title":"J Nutr Biochem"},{"issue":"7","key":"pone.0352642.ref067","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1093\/jn\/129.7.1323","article-title":"Hamsters and guinea pigs differ in their plasma lipoprotein cholesterol distribution when fed diets varying in animal protein, soluble fiber, or cholesterol content","volume":"129","author":"ML Fernandez","year":"1999","journal-title":"J Nutr"},{"issue":"2","key":"pone.0352642.ref068","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.bbadis.2012.11.008","article-title":"High fat diets and pathology in the guinea pig. Atherosclerosis or liver damage?","volume":"1832","author":"P Ye","year":"2013","journal-title":"Biochim Biophys Acta - Mol Basis Dis"},{"key":"pone.0352642.ref069","doi-asserted-by":"crossref","first-page":"100500","DOI":"10.1016\/j.obmed.2023.100500","article-title":"High-fat diet and related obesity provoke neurotoxins and alter neuro-biomarkers involved in Parkinson\u2019s disease","volume":"41","author":"N Batarseh","year":"2023","journal-title":"Obesity Medicine"},{"issue":"4","key":"pone.0352642.ref070","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0191299","article-title":"Brief exposure to obesogenic diet disrupts brain dopamine networks","volume":"13","author":"RL Barry","year":"2018","journal-title":"PLoS One"},{"key":"pone.0352642.ref071","doi-asserted-by":"crossref","first-page":"2649","DOI":"10.3389\/fimmu.2018.02649","article-title":"Inflammatory Links Between High Fat Diets and Diseases","volume":"9","author":"Y Duan","year":"2018","journal-title":"Front Immunol"},{"issue":"1","key":"pone.0352642.ref072","doi-asserted-by":"crossref","first-page":"207","DOI":"10.3390\/ijms21010207","article-title":"High Fat Diet Suppresses Peroxisome Proliferator-Activated Receptors and Reduces Dopaminergic Neurons in the Substantia Nigra","volume":"21","author":"Y-C Kao","year":"2019","journal-title":"Int J Mol Sci"},{"issue":"1","key":"pone.0352642.ref073","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/ijo.2016.183","article-title":"High fat induces acute and chronic inflammation in the hypothalamus: effect of high-fat diet, palmitate and TNF-\u03b1 on appetite-regulating NPY neurons","volume":"41","author":"PS Dalvi","year":"2017","journal-title":"Int J Obes (Lond)"},{"key":"pone.0352642.ref074","doi-asserted-by":"crossref","first-page":"508","DOI":"10.3389\/fendo.2018.00508","article-title":"Early High-Fat Diet Exposure Causes Dysregulation of the Orexin and Dopamine Neuronal Populations in Nonhuman Primates","volume":"9","author":"C True","year":"2018","journal-title":"Front Endocrinol (Lausanne)"},{"issue":"1","key":"pone.0352642.ref075","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.nbd.2011.09.009","article-title":"High-fat diet exacerbates MPTP-induced dopaminergic degeneration in mice","volume":"45","author":"M Bousquet","year":"2012","journal-title":"Neurobiol Dis"},{"issue":"9","key":"pone.0352642.ref076","doi-asserted-by":"crossref","first-page":"7096","DOI":"10.1007\/s12035-016-0193-8","article-title":"Relationship Between Obesity, Alzheimer\u2019s Disease, and Parkinson\u2019s Disease: an Astrocentric View","volume":"54","author":"CA Martin-Jim\u00e9nez","year":"2017","journal-title":"Mol Neurobiol"},{"key":"pone.0352642.ref077","author":"A \u00d6zer","year":"2023"},{"issue":"6676","key":"pone.0352642.ref078","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1038\/33416","article-title":"Mutations in the parkin gene cause autosomal recessive juvenile parkinsonism","volume":"392","author":"T Kitada","year":"1998","journal-title":"Nature"},{"issue":"5674","key":"pone.0352642.ref079","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1126\/science.1096284","article-title":"Hereditary early-onset Parkinson\u2019s disease caused by mutations in PINK1","volume":"304","author":"EM Valente","year":"2004","journal-title":"Science"},{"issue":"4","key":"pone.0352642.ref080","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1093\/hmg\/ddv616","article-title":"PARK2 enhancement is able to compensate mitophagy alterations found in sporadic Alzheimer\u2019s disease","volume":"25","author":"P Mart\u00edn-Maestro","year":"2016","journal-title":"Hum Mol Genet"},{"issue":"10","key":"pone.0352642.ref081","doi-asserted-by":"crossref","first-page":"2938","DOI":"10.1093\/hmg\/ddv056","article-title":"Parkin-mediated mitophagy in mutant hAPP neurons and Alzheimer\u2019s disease patient brains","volume":"24","author":"X Ye","year":"2015","journal-title":"Hum Mol Genet"},{"issue":"5557","key":"pone.0352642.ref082","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1126\/science.1066901","article-title":"Adult-onset primary open-angle glaucoma caused by mutations in optineurin","volume":"295","author":"T Rezaie","year":"2002","journal-title":"Science"},{"issue":"2","key":"pone.0352642.ref083","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/s41419-017-0146-9","article-title":"Overexpression of parkin protects retinal ganglion cells in experimental glaucoma","volume":"9","author":"Y Dai","year":"2018","journal-title":"Cell Death Dis"},{"issue":"2","key":"pone.0352642.ref084","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1083\/jcb.200910140","article-title":"PINK1 stabilized by mitochondrial depolarization recruits Parkin to damaged mitochondria and activates latent Parkin for mitophagy","volume":"189","author":"N Matsuda","year":"2010","journal-title":"J Cell Biol"},{"issue":"1","key":"pone.0352642.ref085","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1000298","article-title":"PINK1 is selectively stabilized on impaired mitochondria to activate Parkin","volume":"8","author":"DP Narendra","year":"2010","journal-title":"PLoS Biol"},{"issue":"10","key":"pone.0352642.ref086","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1101\/gad.262758.115","article-title":"The three \u2019P\u2019s of mitophagy: PARKIN, PINK1, and post-translational modifications","volume":"29","author":"TM Durcan","year":"2015","journal-title":"Genes Dev"},{"issue":"7565","key":"pone.0352642.ref087","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1038\/nature14893","article-title":"The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy","volume":"524","author":"M Lazarou","year":"2015","journal-title":"Nature"},{"issue":"1","key":"pone.0352642.ref088","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1186\/s40478-020-01062-w","article-title":"PINK1\/PARKIN signalling in neurodegeneration and neuroinflammation","volume":"8","author":"PMJ Quinn","year":"2020","journal-title":"Acta Neuropathol Commun"},{"issue":"16","key":"pone.0352642.ref089","doi-asserted-by":"crossref","first-page":"5703","DOI":"10.1073\/pnas.0500617102","article-title":"Mutations in PTEN-induced putative kinase 1 associated with recessive parkinsonism have differential effects on protein stability","volume":"102","author":"A Beilina","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"pone.0352642.ref090","doi-asserted-by":"crossref","first-page":"120080","DOI":"10.1098\/rsob.120080","article-title":"PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65","volume":"2","author":"C Kondapalli","year":"2012","journal-title":"Open Biol"},{"issue":"12","key":"pone.0352642.ref091","doi-asserted-by":"crossref","first-page":"168090","DOI":"10.1016\/j.jmb.2023.168090","article-title":"Structural Mechanisms of Mitochondrial Quality Control Mediated by PINK1 and Parkin","volume":"435","author":"J-F Trempe","year":"2023","journal-title":"J Mol Biol"},{"key":"pone.0352642.ref092","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1038\/ncomms2016","article-title":"PINK1 autophosphorylation upon membrane potential dissipation is essential for Parkin recruitment to damaged mitochondria","volume":"3","author":"K Okatsu","year":"2012","journal-title":"Nat Commun"},{"issue":"1","key":"pone.0352642.ref093","doi-asserted-by":"crossref","DOI":"10.1016\/j.molcel.2021.11.012","article-title":"Mechanism of PINK1 activation by autophosphorylation and insights into assembly on the TOM complex","volume":"82","author":"S Rasool","year":"2022","journal-title":"Mol Cell"},{"key":"pone.0352642.ref094","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.neuroscience.2015.03.017","article-title":"Dysregulation of parkin in the substantia nigra of db\/db and high-fat diet mice","volume":"294","author":"R Khang","year":"2015","journal-title":"Neuroscience"},{"key":"pone.0352642.ref095","first-page":"4822767","article-title":"Dietary High-Fat Promotes Cognitive Impairment by Suppressing Mitophagy","volume":"2023","author":"J Wen","year":"2023","journal-title":"Oxid Med Cell Longev"},{"key":"pone.0352642.ref096","doi-asserted-by":"crossref","first-page":"408","DOI":"10.3389\/fnins.2016.00408","article-title":"Alpha-Synuclein Oligomers-Neurotoxic Molecules in Parkinson\u2019s Disease and Other Lewy Body Disorders","volume":"10","author":"M Ingelsson","year":"2016","journal-title":"Front Neurosci"},{"key":"pone.0352642.ref097","doi-asserted-by":"crossref","first-page":"663","DOI":"10.3889\/oamjms.2022.7940","article-title":"Relationship between Plasma Level of Beta-amyloid, Alpha-synuclein, and Tau Protein with Cognitive Impairment in Parkinson Disease","volume":"10","author":"Y Syafrita","year":"2022","journal-title":"Open Access Maced J Med Sci"},{"issue":"12","key":"pone.0352642.ref098","doi-asserted-by":"crossref","first-page":"1978","DOI":"10.1002\/cne.24468","article-title":"Sensitivity and specificity of phospho-Ser129 \u03b1-synuclein monoclonal antibodies","volume":"526","author":"V Delic","year":"2018","journal-title":"J Comp Neurol"},{"issue":"1","key":"pone.0352642.ref099","doi-asserted-by":"crossref","first-page":"39","DOI":"10.3233\/JPD-160779","article-title":"Implication of Alpha-Synuclein Phosphorylation at S129 in Synucleinopathies: What Have We Learned in the Last Decade?","volume":"6","author":"A Oueslati","year":"2016","journal-title":"J Parkinsons Dis"},{"issue":"5","key":"pone.0352642.ref100","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1080\/1028415X.2020.1831261","article-title":"High fat diet-induced obesity causes a reduction in brain tyrosine hydroxylase levels and non-motor features in rats through metabolic dysfunction, neuroinflammation and oxidative stress","volume":"25","author":"A Bittencourt","year":"2022","journal-title":"Nutr Neurosci"},{"issue":"5","key":"pone.0352642.ref101","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1080\/1028415X.2017.1387720","article-title":"The impact of obesity on brain iron levels and \u03b1-synuclein expression is regionally dependent","volume":"22","author":"J Han","year":"2019","journal-title":"Nutr Neurosci"},{"key":"pone.0352642.ref102","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1093\/brain\/aws360","article-title":"The cerebellum in Parkinson\u2019s disease","volume":"136","author":"T Wu","year":"2013","journal-title":"Brain"},{"issue":"2","key":"pone.0352642.ref103","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1002\/1531-8249(200002)47:2<201::AID-ANA10>3.0.CO;2-F","article-title":"Expression of alpha-synuclein, parkin, and ubiquitin carboxy-terminal hydrolase L1 mRNA in human brain: genes associated with familial Parkinson\u2019s disease","volume":"47","author":"SM Solano","year":"2000","journal-title":"Ann Neurol"},{"issue":"4","key":"pone.0352642.ref104","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/s00401-002-0655-0","article-title":"Alpha-synuclein pathology affecting Bergmann glia of the cerebellum in patients with alpha-synucleinopathies","volume":"105","author":"Y-S Piao","year":"2003","journal-title":"Acta Neuropathol"},{"issue":"5","key":"pone.0352642.ref105","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1096\/fj.07-9348com","article-title":"Genetic variability in the SNCA gene influences \u03b1\u2010synuclein levels in the blood and brain","volume":"22","author":"J Fuchs","year":"2007","journal-title":"The FASEB Journal"},{"issue":"10","key":"pone.0352642.ref106","doi-asserted-by":"crossref","first-page":"3509","DOI":"10.1096\/fj.08-110148","article-title":"Cerebellar alpha-synuclein levels are decreased in Parkinson\u2019s disease and do not correlate with SNCA polymorphisms associated with disease in a Swedish material","volume":"22","author":"M Westerlund","year":"2008","journal-title":"FASEB J"},{"key":"pone.0352642.ref107","doi-asserted-by":"crossref","first-page":"101892","DOI":"10.1016\/j.jchemneu.2020.101892","article-title":"Cerebral and cerebellar histological changes in the rat animal model of rotenone induced parkinsonism can be ameliorated by bone marrow derived stem cell conditioned media","volume":"111","author":"SA Abdelwahab","year":"2021","journal-title":"J Chem Neuroanat"},{"key":"pone.0352642.ref108","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.molbrainres.2004.12.013","article-title":"Differential expression of dopamine D2 and D4 receptor and tyrosine hydroxylase mRNA in mice prone, or resistant, to chronic high-fat diet-induced obesity","volume":"135","author":"X-F Huang","year":"2005","journal-title":"Brain Res Mol Brain Res"},{"issue":"7422","key":"pone.0352642.ref109","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1016\/S0140-6736(65)90062-0","article-title":"Inhibition of catecholamine synthesis in man with alpha-methyl-tyrosine, an inhibitor of tyrosine hydroxylase","volume":"2","author":"A Sjoerdsma","year":"1965","journal-title":"Lancet"},{"issue":"4","key":"pone.0352642.ref110","doi-asserted-by":"crossref","first-page":"395","DOI":"10.2174\/187152712800792785","article-title":"A synopsis on the role of tyrosine hydroxylase in Parkinson\u2019s disease","volume":"11","author":"S Tabrez","year":"2012","journal-title":"CNS Neurol Disord Drug Targets"},{"issue":"4","key":"pone.0352642.ref111","doi-asserted-by":"crossref","first-page":"350","DOI":"10.2174\/187152712800792901","article-title":"Overview of tyrosine hydroxylase in Parkinson\u2019s disease","volume":"11","author":"Y Zhu","year":"2012","journal-title":"CNS Neurol Disord Drug Targets"},{"issue":"5","key":"pone.0352642.ref112","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1203\/01.PDR.0000119365.21770.45","article-title":"Stereospecific regulation of tyrosine hydroxylase and proenkephalin genes by short-chain fatty acids in rat PC12 cells","volume":"55","author":"P Mally","year":"2004","journal-title":"Pediatr Res"},{"issue":"1","key":"pone.0352642.ref113","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.molbrainres.2005.09.002","article-title":"Short chain fatty acids regulate tyrosine hydroxylase gene expression through a cAMP-dependent signaling pathway","volume":"142","author":"M DeCastro","year":"2005","journal-title":"Brain Res Mol Brain Res"},{"key":"pone.0352642.ref114","doi-asserted-by":"crossref","first-page":"552","DOI":"10.3389\/fnins.2018.00552","article-title":"Palmitic Acid-Enriched Diet Increases \u03b1-Synuclein and Tyrosine Hydroxylase Expression Levels in the Mouse Brain","volume":"12","author":"J Schommer","year":"2018","journal-title":"Front Neurosci"},{"issue":"1","key":"pone.0352642.ref115","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.nbd.2004.01.015","article-title":"Low-density lipoprotein receptor-knockout mice display impaired spatial memory associated with a decreased synaptic density in the hippocampus","volume":"16","author":"M Mulder","year":"2004","journal-title":"Neurobiol Dis"},{"issue":"38","key":"pone.0352642.ref116","doi-asserted-by":"crossref","first-page":"15502","DOI":"10.1073\/pnas.1206446109","article-title":"Low-density lipoprotein receptor overexpression enhances the rate of brain-to-blood A\u03b2 clearance in a mouse model of \u03b2-amyloidosis","volume":"109","author":"JM Castellano","year":"2012","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"pone.0352642.ref117","doi-asserted-by":"crossref","first-page":"43","DOI":"10.3233\/JAD-132228","article-title":"Increased susceptibility to amyloid-\u03b2-induced neurotoxicity in mice lacking the low-density lipoprotein receptor","volume":"41","author":"J de Oliveira","year":"2014","journal-title":"J Alzheimers Dis"},{"issue":"3","key":"pone.0352642.ref118","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1038\/nn.3028","article-title":"The toxic A\u03b2 oligomer and Alzheimer\u2019s disease: an emperor in need of clothes","volume":"15","author":"I Benilova","year":"2012","journal-title":"Nat Neurosci"},{"issue":"5580","key":"pone.0352642.ref119","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1126\/science.1072994","article-title":"The amyloid hypothesis of Alzheimer\u2019s disease: progress and problems on the road to therapeutics","volume":"297","author":"J Hardy","year":"2002","journal-title":"Science"},{"issue":"17","key":"pone.0352642.ref120","doi-asserted-by":"crossref","first-page":"13959","DOI":"10.1074\/jbc.M111.288746","article-title":"Low-density lipoprotein receptor represents an apolipoprotein E-independent pathway of A\u03b2 uptake and degradation by astrocytes","volume":"287","author":"JM Basak","year":"2012","journal-title":"J Biol Chem"},{"issue":"2","key":"pone.0352642.ref121","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.bbr.2008.03.036","article-title":"Increased locomotor activity in mice lacking the low-density lipoprotein receptor","volume":"191","author":"GA Elder","year":"2008","journal-title":"Behav Brain Res"},{"issue":"2","key":"pone.0352642.ref122","doi-asserted-by":"crossref","first-page":"495","DOI":"10.3233\/JAD-2012-120541","article-title":"Age-related cognitive decline in hypercholesterolemic LDL receptor knockout mice (LDLr\u2212\/\u2212): evidence of antioxidant imbalance and increased acetylcholinesterase activity in the prefrontal cortex","volume":"32","author":"ELG Moreira","year":"2012","journal-title":"J Alzheimers Dis"},{"key":"pone.0352642.ref123","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1186\/1476-511X-13-175","article-title":"LDL receptor knock-out mice show impaired spatial cognition with hippocampal vulnerability to apoptosis and deficits in synapses","volume":"13","author":"S Wang","year":"2014","journal-title":"Lipids Health Dis"},{"key":"pone.0352642.ref124","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.neuroscience.2011.09.009","article-title":"Positive correlation between elevated plasma cholesterol levels and cognitive impairments in LDL receptor knockout mice: relevance of cortico-cerebral mitochondrial dysfunction and oxidative stress","volume":"197","author":"J De Oliveira","year":"2011","journal-title":"Neuroscience"},{"issue":"6","key":"pone.0352642.ref125","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1016\/S0022-2275(20)41127-7","article-title":"Regulation of guinea pig very low density lipoprotein secretion rates by dietary fat saturation","volume":"36","author":"G Abdel-Fattah","year":"1995","journal-title":"J Lipid Res"},{"key":"pone.0352642.ref126","doi-asserted-by":"crossref","DOI":"10.1093\/ajcn\/65.5.1617S","article-title":"Dietary fat saturation effects on low-density-lipoprotein concentrations and metabolism in various animal models","volume":"65","author":"RJ Nicolosi","year":"1997","journal-title":"Am J Clin Nutr"},{"key":"pone.0352642.ref127","doi-asserted-by":"crossref","first-page":"4591","DOI":"10.12659\/MSM.903636","article-title":"Differences in ABCA1 R219K Polymorphisms and Serum Indexes in Alzheimer and Parkinson Diseases in Northern China","volume":"23","author":"L Ya","year":"2017","journal-title":"Med Sci Monit"},{"key":"pone.0352642.ref128","doi-asserted-by":"crossref","first-page":"940118","DOI":"10.3389\/fneur.2022.940118","article-title":"Lipid-lowering drug targets and Parkinson\u2019s disease: A sex-specific Mendelian randomization study","volume":"13","author":"Y Zhao","year":"2022","journal-title":"Front Neurol"},{"key":"pone.0352642.ref129","article-title":"Low LDL cholesterol,PCSK9andHMGCRgenetic variation, and risk of Alzheimer\u2019s disease and Parkinson\u2019s disease: Mendelian randomisation study","author":"M Benn","year":"2017","journal-title":"BMJ"},{"issue":"6","key":"pone.0352642.ref130","article-title":"A Mendelian Randomization Approach Using 3-HMG-Coenzyme-A Reductase Gene Variation to Evaluate the Association of Statin-Induced Low-Density Lipoprotein Cholesterol Lowering With Noncardiovascular Disease Phenotypes","volume":"4","author":"G Liu","year":"2021","journal-title":"JAMA Netw Open"},{"issue":"5","key":"pone.0352642.ref131","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1002\/ana.25880","article-title":"Evaluating Lipid-Lowering Drug Targets for Parkinson\u2019s Disease Prevention with Mendelian Randomization","volume":"88","author":"DM Williams","year":"2020","journal-title":"Ann Neurol"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0352642","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T17:38:55Z","timestamp":1782409135000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0352642"}},"subtitle":[],"editor":[{"given":"David","family":"Chau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,6,25]]},"references-count":131,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6,25]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0352642","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,25]]}}}