{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T19:50:42Z","timestamp":1782849042831,"version":"3.54.5"},"reference-count":31,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["301555\/2023-0"],"award-info":[{"award-number":["301555\/2023-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-02021-21 and BPD-00807-22"],"award-info":[{"award-number":["APQ-02021-21 and BPD-00807-22"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-05079-23 and RED-00067-23"],"award-info":[{"award-number":["APQ-05079-23 and RED-00067-23"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Serological methods are valuable diagnostic tools for bovine brucellosis, a zoonotic infectious disease with a worldwide distribution. Currently employed diagnostic methods utilize crude bacterial extract or regions of LPS, a molecule that can exhibit cross-reactions with other infectious agents. The goal of this study was to develop an indirect enzyme-linked immunosorbent assay (ELISAi) using synthetic peptides or a multi-epitope protein based on\n                    <jats:italic>in silico-<\/jats:italic>\n                    predicted B cell epitopes. Peptides were synthesized on a cellulose membrane using spot synthesis, and immunoblots were performed to evaluate reactivity by densitometry against bovine sera of interest. Peptides reacting with serum from positive cattle and non-reactive to the serum from negative controls were selected, synthesized in a soluble form, and used as antigens for the development of the ELISAi. Two peptides (P1 and P2) were selected and, after standardization of the ELISAi, positive (25) and negative (175) samples were tested, resulting in a sensitivity of 84% (21\/25) and specificity of 83.43% (146\/175). The sequence of the two peptides in replicates with spacers were inserted into a multi-epitope protein, which was also used as antigen in an ELISAi resulting in a sensitivity of 72% (18\/25) and a specificity of 61.71% (108\/175). This study provided a preliminary analytical assessment of\n                    <jats:italic>in silico<\/jats:italic>\n                    -predicted epitopes for developing novel serologic diagnostic tests.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0352788","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T19:01:07Z","timestamp":1782846067000},"page":"e0352788","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["In silico-predicted B-cell epitopes for bovine brucellosis serodiagnosis: Preliminary analytical evaluation of synthetic peptide- and multi-epitope protein-based indirect ELISAs"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1685-5348","authenticated-orcid":true,"given":"Lucas dos Reis","family":"de Souza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique Ferreira Silva","family":"Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e2mela Aparecida","family":"Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9681-4284","authenticated-orcid":true,"given":"Tatyane Martins","family":"Cirilo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia Neves","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel Alexandre Pimenta","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"de S\u00e1 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Blasco","year":"1994","journal-title":"Vet Rec"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0352788","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T19:01:15Z","timestamp":1782846075000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0352788"}},"subtitle":[],"editor":[{"given":"Odir 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