{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T18:19:13Z","timestamp":1783621153644,"version":"3.55.0"},"reference-count":81,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Agricultural intensification and the associated increase in pesticide use have raised concerns about impacts on non-target insects. However, fungicides, despite their frequent application, remain poorly studied, particularly for herbivorous species. This is partly caused by the fact that they are considered less harmful than other pesticides. We here investigate the effects of three fungicides commonly used in viticulture, two sulphur- and one fluxapyroxad-based ones, on the cabbage white butterfly (\n                    <jats:italic>Pieris rapae<\/jats:italic>\n                    ) under controlled laboratory conditions. Larvae were exposed to fungicides orally via treated host plants or by direct contact, and mortality and sublethal effects were assessed during development. In our study, oral exposure did not increase mortality relative to controls. Regarding sublethal effects, larvae exposed orally to sulphur-based fungicides exhibited a longer pupal development compared to controls. Additionally, larvae exposed to fluxapyroxad showed shorter development time and higher fat content than those exposed to sulphur-based fungicides. Contact exposure resulted in increased mortality relative to controls, with no significant differences among fungicides. These findings demonstrate that fungicide effects on non-target Lepidoptera are route-dependent, resulting in sublethal impacts via ingestion and lethal effects via contact exposure. Our results thus emphasize the need to consider (1) the dual risk posed by fungicides through both lethal and sublethal effects, (2) exposure route, and (3) fungicide-specific responses when assessing fungicide risks, particularly in agricultural systems where non-target Lepidoptera may occur. Detrimental effects of direct contact can be minimized through the timing of fungicide applications, thereby reducing the route-dependent risks identified in this study.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0353528","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T17:41:49Z","timestamp":1783618909000},"page":"e0353528","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exposure route mediates toxicological effects of sulphur and fluxapyroxad fungicides in a non-target butterfly"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-2819-1623","authenticated-orcid":true,"given":"Mine","family":"Yilmazer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Bakenecker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Busch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farina","family":"Fuchs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Hitzler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2871-246X","authenticated-orcid":true,"given":"Klaus","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"pone.0353528.ref001","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1002\/fee.1450","article-title":"Synthetic 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Zhang","year":"2025","journal-title":"Insects"},{"issue":"1","key":"pone.0353528.ref029","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1007\/s11356-014-3471-x","article-title":"Effects of neonicotinoids and fipronil on non-target invertebrates","volume":"22","author":"LW Pisa","year":"2015","journal-title":"Environ Sci Pollut Res Int"},{"key":"pone.0353528.ref030","author":"K Fischer","year":"2026","journal-title":"Toxicological effects of a difenoconazole fungicide on a non-target butterfly"},{"issue":"1","key":"pone.0353528.ref031","doi-asserted-by":"crossref","first-page":"14490","DOI":"10.1038\/s41598-020-71211-7","article-title":"Larval pesticide exposure impacts monarch butterfly performance","volume":"10","author":"P Olaya-Arenas","year":"2020","journal-title":"Sci Rep"},{"issue":"3","key":"pone.0353528.ref032","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1111\/j.1755-0238.2006.tb00060.x","article-title":"An evaluation of biological and abiotic controls 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Vineyard trials","volume":"12","author":"P CRISP","year":"2006","journal-title":"Aust J Grape Wine Res"},{"key":"pone.0353528.ref033","article-title":"Conclusion on the peer review of the pesticide risk assessment of the active substance sulfur","volume":"7","author":"EFSA EFSA","year":"2009","journal-title":"EFSA Journal"},{"issue":"1","key":"pone.0353528.ref034","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/s10493-008-9191-5","article-title":"Toxicity of six novel fungicides and sulphur to Galendromus occidentalis (Acari: Phytoseiidae)","volume":"47","author":"NJ Bostanian","year":"2009","journal-title":"Exp Appl Acarol"},{"issue":"11","key":"pone.0353528.ref035","doi-asserted-by":"crossref","first-page":"825","DOI":"10.3390\/insects11110825","article-title":"Side Effects of Sulfur Dust on the European Grapevine Moth Lobesia botrana and the Predatory Mite Kampimodromus aberrans in Vineyards","volume":"11","author":"F Tacoli","year":"2020","journal-title":"Insects"},{"issue":"7","key":"pone.0353528.ref036","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1094\/PDIS-01-17-0067-RE","article-title":"Resistance to the SDHI Fungicides Boscalid, Fluopyram, Fluxapyroxad, and Penthiopyrad in Botrytis cinerea from Commercial Strawberry Fields in Spain","volume":"101","author":"D Fern\u00e1ndez-Ortu\u00f1o","year":"2017","journal-title":"Plant Dis"},{"issue":"4","key":"pone.0353528.ref037","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1094\/PDIS-07-22-1615-RE","article-title":"Fluxapyroxad Resistance Mechanisms in Sclerotinia sclerotiorum","volume":"107","author":"Y Wang","year":"2023","journal-title":"Plant Dis"},{"key":"pone.0353528.ref038","doi-asserted-by":"crossref","first-page":"108219","DOI":"10.1016\/j.envint.2023.108219","article-title":"SDHi fungicides: An example of mitotoxic pesticides targeting the succinate dehydrogenase complex","volume":"180","author":"C Duarte Hospital","year":"2023","journal-title":"Environ Int"},{"issue":"3","key":"pone.0353528.ref039","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s10646-024-02849-6","article-title":"Ecotoxicological impact of succinate dehydrogenase inhibitor (SDHI) fungicides on non-targeted organisms: a review","volume":"34","author":"S Dwivedi","year":"2025","journal-title":"Ecotoxicology"},{"key":"pone.0353528.ref040","doi-asserted-by":"crossref","first-page":"138175","DOI":"10.1016\/j.jhazmat.2025.138175","article-title":"Assessing the impact of co-exposure to succinate dehydrogenase inhibitor (SDHI) fungicides and the intestinal parasite Nosema ceranae in the honey bee Apis mellifera","volume":"492","author":"J Gilbert","year":"2025","journal-title":"J Hazard Mater"},{"key":"pone.0353528.ref041","doi-asserted-by":"crossref","first-page":"176773","DOI":"10.1016\/j.scitotenv.2024.176773","article-title":"Derivation of ecological safety thresholds and risk assessment of new SDHI fungicides in farmland system","volume":"954","author":"J Zhang","year":"2024","journal-title":"Sci Total Environ"},{"key":"pone.0353528.ref042","doi-asserted-by":"crossref","first-page":"135911","DOI":"10.1016\/j.jhazmat.2024.135911","article-title":"Fluxapyroxad induced toxicity of earthworms: Insights from multi-level experiments and molecular simulation studies","volume":"480","author":"L Zhang","year":"2024","journal-title":"J Hazard Mater"},{"key":"pone.0353528.ref043","first-page":"169","article-title":"Evaluation of some insecticides against Pieris rapae (L., 1758) and Bemisia tabaci (Genn., 1889): impacts on natural enemies and biochemical response","volume":"40","author":"MAS Ahmed","year":"2025","journal-title":"Punjab Univ J Zool"},{"key":"pone.0353528.ref044","first-page":"180","article-title":"Toxicity and efficacy of chlorantraniliprole on Pieris rapae (Linnaeus) (Lepidoptera: Pieridae) on cabbage","volume":"9","author":"Q Su","year":"2017","journal-title":"J Agric Sci"},{"issue":"5","key":"pone.0353528.ref045","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1111\/j.0906-7590.2004.03906.x","article-title":"Landscape effects on butterfly assemblages in an agricultural region","volume":"27","author":"K Bergman","year":"2004","journal-title":"Ecography"},{"issue":"3","key":"pone.0353528.ref046","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0006-3207(00)00182-8","article-title":"Butterfly diversity loss in Flanders (north Belgium): Europe\u2019s worst case scenario?","volume":"99","author":"D Maes","year":"2001","journal-title":"Biological Conservation"},{"issue":"40","key":"pone.0353528.ref047","doi-asserted-by":"crossref","first-page":"20015","DOI":"10.1073\/pnas.1907492116","article-title":"Global invasion history of the agricultural pest butterfly Pieris rapae revealed with genomics and citizen science","volume":"116","author":"SF Ryan","year":"2019","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pone.0353528.ref048","doi-asserted-by":"crossref","first-page":"243","DOI":"10.2307\/1459","article-title":"The biology of the small white butterfly (Pieris rapae), with special reference to the factors controlling its abundance","volume":"9","author":"OW Richards","year":"1940","journal-title":"J Anim Ecol"},{"key":"pone.0353528.ref049","volume-title":"Klimadiagramm Koblenz-Horchheim (mean 1971\u20132000). Klimadiagramme.de","year":"2013"},{"issue":"24","key":"pone.0353528.ref050","doi-asserted-by":"crossref","first-page":"17963","DOI":"10.1002\/ece3.8392","article-title":"Population-specific responses of an insect herbivore to variation in host-plant quality","volume":"11","author":"J Kuczyk","year":"2021","journal-title":"Ecol Evol"},{"key":"pone.0353528.ref051","volume-title":"Helicopterapplication plan for the 2025 spraying season","author":"ADD S and SDR-P","year":"2023"},{"key":"pone.0353528.ref052","volume-title":"List of plant protection products approved for application by aircraft (status: April 2025)","author":"BVL FO of CP and FS","year":"2025"},{"issue":"12","key":"pone.0353528.ref053","doi-asserted-by":"crossref","first-page":"3138","DOI":"10.1890\/02-0733","article-title":"PLASTICITY IN BUTTERFLY EGG SIZE: WHY LARGER OFFSPRING AT LOWER TEMPERATURES?","volume":"84","author":"K Fischer","year":"2003","journal-title":"Ecology"},{"key":"pone.0353528.ref054","volume-title":"R: a language and environment for statistical computing","author":"R Core Team","year":"2023"},{"key":"pone.0353528.ref055","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v067.i01","article-title":"Fitting linear mixed-effects models using lme4","volume":"67","author":"D Bates","year":"2015","journal-title":"J Stat Softw"},{"key":"pone.0353528.ref056","volume-title":"Emmeans: Estimated marginal means, aka least-squares means","author":"R Lenth","year":"2025"},{"key":"pone.0353528.ref057","author":"F Hartig","year":"2024"},{"key":"pone.0353528.ref058","volume-title":"An R companion to applied regression","author":"J Fox","year":"2019","edition":"3"},{"key":"pone.0353528.ref059","volume-title":"Survival: a package for survival analysis in R","author":"TM Therneau","year":"2024"},{"key":"pone.0353528.ref060","volume-title":"Survminer: drawing survival curves using \u201cggplot2\u201d","author":"A Kassambara","year":"2025"},{"key":"pone.0353528.ref061","author":"Fungicide Resistance Action Committee (FRAC)","year":"2026"},{"issue":"3","key":"pone.0353528.ref062","doi-asserted-by":"crossref","first-page":"576","DOI":"10.3390\/antiox11030576","article-title":"Elemental Sulfur Inhibits Yeast Growth via Producing Toxic Sulfide and Causing Disulfide Stress","volume":"11","author":"T Wang","year":"2022","journal-title":"Antioxidants (Basel)"},{"issue":"3","key":"pone.0353528.ref063","first-page":"395","article-title":"Sulfur and sulfur compounds in plant defence","volume":"7","author":"ID Nwachukwu","year":"2012","journal-title":"Nat Prod Commun"},{"issue":"5","key":"pone.0353528.ref064","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/S0169-5347(01)02124-3","article-title":"Compensation for a bad start: grow now, pay later?","volume":"16","author":"NB Metcalfe","year":"2001","journal-title":"Trends Ecol Evol"},{"issue":"3","key":"pone.0353528.ref065","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1111\/1365-2656.12034","article-title":"Plastic larval development in a butterfly has complex environmental and genetic causes and consequences for population dynamics","volume":"82","author":"M Saastamoinen","year":"2013","journal-title":"J Anim Ecol"},{"issue":"4","key":"pone.0353528.ref066","first-page":"987","article-title":"The Slow-Growth--High-Mortality Hypothesis: A Test Using the Cabbage Butterfly","volume":"78","author":"B Benrey","year":"1997","journal-title":"Ecology"},{"issue":"1","key":"pone.0353528.ref067","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1111\/1365-2656.12594","article-title":"Sensitivity of UK butterflies to local climatic extremes: which life stages are most at risk?","volume":"86","author":"O McDermott Long","year":"2017","journal-title":"J Anim Ecol"},{"key":"pone.0353528.ref068","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1093\/zoolinnean\/zlac052","article-title":"Butterfly\u2013parasitoid\u2013host plant interactions in Western Palaearctic Hesperiidae: a DNA barcoding reference library","volume":"196","author":"E Toro-Delgado","year":"2022","journal-title":"Zool J Linn Soc"},{"issue":"5","key":"pone.0353528.ref069","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1093\/ee\/nvac055","article-title":"Ecological predictors of pupal survival in a common North American butterfly","volume":"51","author":"A Brackley","year":"2022","journal-title":"Environ Entomol"},{"key":"pone.0353528.ref070","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1146\/annurev-ento-112408-085356","article-title":"Insect fat body: energy, metabolism, and regulation","volume":"55","author":"EL Arrese","year":"2010","journal-title":"Annu Rev Entomol"},{"issue":"3","key":"pone.0353528.ref071","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/s00442-005-0076-6","article-title":"Larval food limitation in butterflies: effects on adult resource allocation and fitness","volume":"144","author":"CL Boggs","year":"2005","journal-title":"Oecologia"},{"key":"pone.0353528.ref072","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.ibmb.2018.10.005","article-title":"How do oral insecticidal compounds cross the insect midgut epithelium?","volume":"103","author":"S Denecke","year":"2018","journal-title":"Insect Biochem Mol Biol"},{"key":"pone.0353528.ref073","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.cois.2020.11.004","article-title":"The role of cytochrome P450-mediated detoxification in insect adaptation to xenobiotics","volume":"43","author":"K Lu","year":"2021","journal-title":"Curr Opin Insect Sci"},{"issue":"1","key":"pone.0353528.ref074","article-title":"Identification of putative cytochrome P450 monooxygenase genes from the small white butterfly, Pieris rapae (Lepidoptera: Pieridae), and their response to insecticides","volume":"98","author":"S Liu","year":"2018","journal-title":"Arch Insect Biochem Physiol"},{"key":"pone.0353528.ref075","doi-asserted-by":"crossref","first-page":"127037","DOI":"10.1016\/j.chemosphere.2020.127037","article-title":"Fluxapyroxad induces developmental delay in zebrafish (Danio rerio)","volume":"256","author":"W Li","year":"2020","journal-title":"Chemosphere"},{"issue":"2","key":"pone.0353528.ref076","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1007\/BF00363830","article-title":"Why do males emerge before females? protandry as a mating strategy in male and female butterflies","volume":"52","author":"T Fagerstr\u00f6m","year":"1982","journal-title":"Oecologia"},{"key":"pone.0353528.ref077","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/eea.12305","article-title":"Fat and sassy: factors underlying male 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