{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:09:56Z","timestamp":1789754996632,"version":"4.0.1"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Maternal diabetes mellitus (DM) is a known risk factor for offspring metabolic dysfunction, predisposing them to DM later in life. While this association is established, the molecular mechanisms underlying the intergenerational transmission of DM risk are not fully understood. This study investigated the effects of in utero exposure to maternal DM on circulating levels of asprosin in offspring, aiming to clarify the potential role of asprosin in this pathological cascade. Twelve pregnant Wistar rats were randomized into two groups: a streptozotocin (STZ)-induced diabetic group (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096) and a non-diabetic control group (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096). DM was confirmed via blood glucose measurements (&gt;250 mg\/dL) 72 hours post-STZ administration. At 16 weeks postnatal, six male offspring per group were euthanized for plasma collection. Asprosin levels were measured using a commercial ELISA kit. Statistical analyses were performed to compare group means and determine the relationship between asprosin and variables. Maternal asprosin levels were significantly higher in the DM group compared to controls (330.70\u2009\u00b1\u200978.13 vs. 244.00\u2009\u00b1\u200921.54 pg\/mL,\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.02). However, offspring of both groups showed comparable circulating asprosin concentrations (234.50\u2009\u00b1\u200910.60 vs 235.00\u2009\u00b1\u20097.23 pg\/mL,\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.92). In contrast to diabetic mothers, in whom asprosin was significantly correlated with atherogenic lipid parameters and glucose homeostasis indices, no significant correlations were observed between asprosin and these parameters in offspring of either group. These preliminary findings suggest that asprosin may not be a critical mediator in the intergenerational transmission of diabetes-associated metabolic dysfunction.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0354870","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T17:42:28Z","timestamp":1789753348000},"page":"e0354870","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the link between asprosin and glucose and lipid dysregulation in offspring of diabetic mothers in a rat model"],"prefix":"10.1371","volume":"21","author":[{"given":"Hamidreza","family":"Ahmadasadi","sequence":"first","affiliation":[{"name":"TeMS.C., Islamic Azad University","place":["Tehran, Iran"],"department":["Department of Biochemistry and Biophysics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peyman","family":"Rasouli Emamgholi","sequence":"additional","affiliation":[{"name":"TeMS.C., Islamic Azad University","place":["Tehran, Iran"],"department":["Department of Biochemistry and Biophysics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Issa","family":"Layali","sequence":"additional","affiliation":[{"name":"TeMS.C., Islamic Azad University","place":["Tehran, Iran"],"department":["Department of Biochemistry and Biophysics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina","family":"Kianfar","sequence":"additional","affiliation":[{"name":"School of Medicine, Iran University of Medical Sciences","place":["Tehran, Iran"],"department":["Department of Physiology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahriyar","family":"Tork","sequence":"additional","affiliation":[{"name":"School of Medicine, Iran University of Medical Sciences","place":["Tehran, Iran"],"department":["Department of Anatomy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mobina","family":"Jafarpour","sequence":"additional","affiliation":[{"name":"School of Medicine, Iran University of Medical Sciences","place":["Tehran, Iran"],"department":["Department of Biochemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masoume","family":"Aliabadi","sequence":"additional","affiliation":[{"name":"School of Medicine, Iran University of Medical Sciences","place":["Tehran, Iran"],"department":["Department of Biochemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6323-8129","authenticated-orcid":true,"given":"Hafez","family":"Heydari","sequence":"additional","affiliation":[{"name":"School of Medicine, Iran University of Medical Sciences","place":["Tehran, Iran"],"department":["Department of Biochemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"pone.0354870.ref001","article-title":"2. Diagnosis and Classification of Diabetes: Standards of Care in Diabetes-2024","volume":"47","author":"American Diabetes Association Professional Practice Committee","year":"2024","journal-title":"Diabetes Care"},{"issue":"1","key":"pone.0354870.ref002","doi-asserted-by":"crossref","first-page":"26","DOI":"10.5853\/jos.2022.02306","article-title":"Diabetes and stroke: what are the connections?","volume":"25","author":"O Mosenzon","year":"2023","journal-title":"J Stroke"},{"key":"pone.0354870.ref003","doi-asserted-by":"crossref","first-page":"1265372","DOI":"10.3389\/fendo.2023.1265372","article-title":"Diabetic peripheral neuropathy: pathogenetic mechanisms and treatment","volume":"14","author":"J Zhu","year":"2024","journal-title":"Front Endocrinol (Lausanne)"},{"issue":"10","key":"pone.0354870.ref004","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1007\/s11892-021-01404-5","article-title":"Association between diabetes, diabetic retinopathy, and glaucoma","volume":"21","author":"Y Li","year":"2021","journal-title":"Curr Diab Rep"},{"key":"pone.0354870.ref005","doi-asserted-by":"crossref","first-page":"3086167","DOI":"10.1155\/2018\/3086167","article-title":"Complications of diabetes 2017","volume":"2018","author":"K Papatheodorou","year":"2018","journal-title":"J Diabetes Res"},{"issue":"1","key":"pone.0354870.ref006","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1186\/s13690-021-00632-1","article-title":"Epidemiological factors for type 2 diabetes mellitus: evidence from the Global Burden of Disease","volume":"79","author":"A Kotwas","year":"2021","journal-title":"Arch Public Health"},{"key":"pone.0354870.ref007","article-title":"International diabetes federation","volume-title":"IDF diabetes atlas","author":"D Atlas","year":"2015","edition":"7"},{"issue":"6","key":"pone.0354870.ref008","article-title":"Mechanisms of early life programming: current knowledge and future directions","volume":"94","author":"JL Tarry-Adkins","year":"2011","journal-title":"Am J Clin Nutr"},{"issue":"6","key":"pone.0354870.ref009","doi-asserted-by":"crossref","first-page":"453","DOI":"10.2174\/1389202920666191009110724","article-title":"Early-life programming of type 2 diabetes mellitus: understanding the association between epigenetics\/genetics and environmental factors","volume":"20","author":"FZ Kadayifci","year":"2019","journal-title":"Curr Genomics"},{"key":"pone.0354870.ref010","doi-asserted-by":"crossref","first-page":"105076","DOI":"10.1155\/2011\/105076","article-title":"Early-life origins of type 2 diabetes: fetal programming of the beta-cell mass","volume":"2011","author":"B Portha","year":"2011","journal-title":"Exp Diabetes Res"},{"issue":"3","key":"pone.0354870.ref011","doi-asserted-by":"crossref","first-page":"236","DOI":"10.3390\/nu9030236","article-title":"Early-life nutritional programming of type 2 diabetes: experimental and quasi-experimental evidence","volume":"9","author":"AM Vaiserman","year":"2017","journal-title":"Nutrients"},{"issue":"9","key":"pone.0354870.ref012","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1089\/dia.2009.0163","article-title":"Obesity and metabolic abnormalities in offspring of subjects with diabetes mellitus","volume":"12","author":"EP Praveen","year":"2010","journal-title":"Diabetes Technol Ther"},{"issue":"5","key":"pone.0354870.ref013","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1159\/000448076","article-title":"The cross-link between adipokines, insulin resistance and obesity in offspring of diabetic pregnancies","volume":"86","author":"L Bozkurt","year":"2016","journal-title":"Horm Res Paediatr"},{"key":"pone.0354870.ref014","first-page":"596060","article-title":"Intergenerational cycle of obesity and diabetes: how can we reduce the burdens of these conditions on the health of future generations?","volume":"2011","author":"M-C Battista","year":"2011","journal-title":"Exp Diabetes Res"},{"key":"pone.0354870.ref015","doi-asserted-by":"crossref","first-page":"170132","DOI":"10.1016\/j.peptides.2019.170132","article-title":"Asprosin in umbilical cord of newborns and maternal blood of gestational diabetes, preeclampsia, severe preeclampsia, intrauterine growth retardation and macrosemic fetus","volume":"120","author":"Y Baykus","year":"2019","journal-title":"Peptides"},{"issue":"8","key":"pone.0354870.ref016","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1055\/s-2007-978776","article-title":"Leptin concentrations in cord blood in normal newborn infants and offspring of diabetic mothers","volume":"31","author":"B Persson","year":"1999","journal-title":"Horm Metab Res"},{"issue":"2","key":"pone.0354870.ref017","first-page":"70","article-title":"Cord blood leptin levels in gestational diabetes","volume":"16","author":"\u00d6 \u015eeng\u00fcl","year":"2010","journal-title":"Gynecol Obstet Reprod Med"},{"issue":"6","key":"pone.0354870.ref018","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0131171","article-title":"Increased maternal and cord blood betatrophin in gestational diabetes","volume":"10","author":"N Wawrusiewicz-Kurylonek","year":"2015","journal-title":"PLoS One"},{"issue":"7","key":"pone.0354870.ref019","doi-asserted-by":"crossref","DOI":"10.1161\/JAHA.116.004973","article-title":"Association of parental obesity and diabetes mellitus with circulating adipokines in nonobese nondiabetic offspring","volume":"6","author":"JP Zachariah","year":"2017","journal-title":"J Am Heart Assoc"},{"issue":"6","key":"pone.0354870.ref020","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1097\/EDE.0b013e3181567ed4","article-title":"Adipokines in human milk and risk of overweight in early childhood: a prospective cohort study","volume":"18","author":"M Weyermann","year":"2007","journal-title":"Epidemiology"},{"issue":"3","key":"pone.0354870.ref021","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0092238","article-title":"Impact of the adipokine adiponectin and the hepatokine fetuin-A on the development of type 2 diabetes: prospective cohort- and cross-sectional phenotyping studies","volume":"9","author":"N Stefan","year":"2014","journal-title":"PLoS One"},{"issue":"3","key":"pone.0354870.ref022","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.cell.2016.02.063","article-title":"Asprosin, a fasting-induced glucogenic protein hormone","volume":"165","author":"C Romere","year":"2016","journal-title":"Cell"},{"key":"pone.0354870.ref023","doi-asserted-by":"crossref","first-page":"129228","DOI":"10.1016\/j.chemosphere.2020.129228","article-title":"Maternal exposure to air pollution and umbilical asprosin concentration, a novel insulin-resistant marker","volume":"268","author":"ZS Hosseini","year":"2021","journal-title":"Chemosphere"},{"key":"pone.0354870.ref024","doi-asserted-by":"crossref","first-page":"9471583","DOI":"10.1155\/2018\/9471583","article-title":"Plasma asprosin concentrations are increased in individuals with glucose dysregulation and correlated with insulin resistance and first-phase insulin secretion","volume":"2018","author":"Y Wang","year":"2018","journal-title":"Mediators Inflamm"},{"key":"pone.0354870.ref025","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1186\/s13098-020-00564-w","article-title":"Serum concentration of asprosin in new-onset type 2 diabetes","volume":"12","author":"S Naiemian","year":"2020","journal-title":"Diabetol Metab Syndr"},{"issue":"11","key":"pone.0354870.ref026","doi-asserted-by":"crossref","first-page":"20888","DOI":"10.1002\/jcp.28694","article-title":"Asprosin attenuates insulin signaling pathway through PKC\u03b4-activated ER stress and inflammation in skeletal muscle","volume":"234","author":"TW Jung","year":"2019","journal-title":"J Cell Physiol"},{"issue":"2","key":"pone.0354870.ref027","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1042\/BJ20100414","article-title":"Epigenetic changes and alteration of Fbn1 and Col3A1 gene expression under hyperglycaemic and hyperinsulinaemic conditions","volume":"432","author":"AB Gaikwad","year":"2010","journal-title":"Biochem J"},{"key":"pone.0354870.ref028","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.cca.2017.10.034","article-title":"Circulating asprosin concentrations are increased in type 2 diabetes mellitus and independently associated with fasting glucose and triglyceride","volume":"489","author":"L Zhang","year":"2019","journal-title":"Clin Chim Acta"},{"key":"pone.0354870.ref029","doi-asserted-by":"crossref","first-page":"2515","DOI":"10.2147\/DMSO.S424651","article-title":"Association between plasma asprosin levels and gestational diabetes mellitus","volume":"16","author":"\u0130B Boz","year":"2023","journal-title":"Diabetes Metab Syndr Obes"},{"key":"pone.0354870.ref030","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.placenta.2020.02.004","article-title":"Continuous elevation of plasma asprosin in pregnant women complicated with gestational diabetes mellitus: a nested case-control study","volume":"93","author":"L Zhong","year":"2020","journal-title":"Placenta"},{"key":"pone.0354870.ref031","doi-asserted-by":"crossref","first-page":"101502","DOI":"10.1016\/j.cegh.2023.101502","article-title":"Circulating asprosin levels in type 2 diabetes mellitus: a systematic review and meta-analysis","volume":"25","author":"RK Mahat","year":"2024","journal-title":"Clin Epidemiol Glob Health"},{"key":"pone.0354870.ref032","doi-asserted-by":"crossref","first-page":"155477","DOI":"10.1016\/j.cyto.2021.155477","article-title":"Asprosin serum levels and glucose homeostasis in children with obesity","volume":"142","author":"D Corica","year":"2021","journal-title":"Cytokine"},{"issue":"4","key":"pone.0354870.ref033","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1111\/ped.14176","article-title":"Increased serum circulating asprosin levels in children with obesity","volume":"62","author":"E S\u00fcnnet\u00e7i Silistre","year":"2020","journal-title":"Pediatr Int"},{"issue":"4","key":"pone.0354870.ref034","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1159\/000500523","article-title":"Decreased circulating levels of asprosin in obese children","volume":"91","author":"W Long","year":"2019","journal-title":"Horm Res Paediatr"},{"issue":"4","key":"pone.0354870.ref035","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1159\/000503808","article-title":"Serum asprosin concentrations are increased and associated with insulin resistance in children with obesity","volume":"75","author":"M Wang","year":"2019","journal-title":"Ann Nutr Metab"},{"issue":"3","key":"pone.0354870.ref036","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1111\/j.1476-5381.2012.01911.x","article-title":"The use of animal models in diabetes research","volume":"166","author":"AJF King","year":"2012","journal-title":"Br J Pharmacol"},{"issue":"4","key":"pone.0354870.ref037","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1111\/j.1464-5491.2005.01499.x","article-title":"Animal models of diabetes mellitus","volume":"22","author":"DA Rees","year":"2005","journal-title":"Diabet Med"},{"issue":"4","key":"pone.0354870.ref038","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1093\/ilar.43.4.244","article-title":"Guidelines for the design and statistical analysis of experiments using laboratory animals","volume":"43","author":"MFW Festing","year":"2002","journal-title":"ILAR J"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0354870","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T17:42:34Z","timestamp":1789753354000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0354870"}},"subtitle":[],"editor":[{"given":"Mar\u00eda Teresa","family":"Llin\u00e1s","sequence":"first","affiliation":[{"name":"Universidad de Murcia","place":["SPAIN"]}],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":38,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0354870","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,18]]}}}