{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T17:55:52Z","timestamp":1785779752981,"version":"3.56.0"},"reference-count":75,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Professor SM\u2019s Australian Research Council (ARC) Linkage Project","award":["LP180100001"],"award-info":[{"award-number":["LP180100001"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    A quantitative polymerase chain reaction (qPCR) assay was developed for the species-specific detection and quantification of\n                    <jats:italic>Gambierdiscus holmesii<\/jats:italic>\n                    , recently reported to produce known ciguatoxin (CTX) analogues in north Queensland. The assay was designed to target the ITS region and calibrated using synthetic gBlocks standards and cell-based standard curves to enable accurate quantification. The\n                    <jats:italic>G. holmesii<\/jats:italic>\n                    -specific qPCR assay demonstrated high specificity, efficiency, and sensitivity, with no cross-reactivity observed against closely related\n                    <jats:italic>Gambierdiscus<\/jats:italic>\n                    species. The assay was subsequently applied to environmental samples collected from Heron Island (Great Barrier Reef, Queensland), detecting\n                    <jats:italic>G. holmesii<\/jats:italic>\n                    in 5 out of 12 sites. This newly developed molecular tool enhances the capacity to monitor\n                    <jats:italic>G. holmesii<\/jats:italic>\n                    in the environment and supports improved surveillance of ciguatoxin-producing\n                    <jats:italic>Gambierdiscus<\/jats:italic>\n                    species in Australian waters, enabling earlier detection and more effective management of ciguatera poisoning risks to public health and fisheries.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0355213","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T17:30:32Z","timestamp":1785778232000},"page":"e0355213","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a quantitative PCR assay to detect Gambierdiscus holmesii, a ciguatoxin producing species from Australian waters"],"prefix":"10.1371","volume":"21","author":[{"given":"Caroline","family":"Dornelles de Azevedo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2219-8145","authenticated-orcid":true,"given":"Greta","family":"Gaiani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Tesoriero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsty F.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7096-1307","authenticated-orcid":true,"given":"Shauna 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