{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T18:13:56Z","timestamp":1788459236324,"version":"build-2803163510"},"reference-count":110,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    The actin-binding protein synaptopodin (Synpo) regulates the cytoskeleton and organization of the endoplasmic reticulum, thereby amplifying intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling. Knockout (KO) mouse models have been used to study the role of Synpo in kidney and brain functions, where it supports stress fiber formation, as well as long-term potentiation (LTP) and learning, respectively. Here, we generated\n                    <jats:italic>Synpo<\/jats:italic>\n                    KO rats using CRISPR-Cas9, and show they are viable but have reduced body weight after postnatal days 35\u201345, along with shorter limb bone length. Their basal kidney function is normal into early adulthood. Serial section electron microscopy from\n                    <jats:italic>Synpo<\/jats:italic>\n                    KO rat hippocampus reveals the absence of the spine apparatus in dendrites and cisternal organelle in the axon initial segment (AIS), two Synpo-dependent specializations of smooth endoplasmic reticulum. The AIS in KO was still innervated by inhibitory synapses despite the total loss of the cisternal organelle.\n                    <jats:italic>Synpo<\/jats:italic>\n                    KO rats also showed reduced LTP. Previously unknown KO effect of Synpo on body stature could have an inadvertent impact on behavioral outcomes. Furthermore, rats have a well-defined developmental onset of LTP, compared to the variable onset of LTP in mice. This, combined with known species differences in behavior, makes our KO rat model a valuable resource for assessing the role of Synpo in development, learning, synaptic plasticity, and a wide range of other biological functions.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0355625","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T17:24:13Z","timestamp":1788456253000},"page":"e0355625","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synaptopodin KO rat for assessing the dendritic spine apparatus and axonal cisternal organelle in synaptic plasticity, development, and behavior"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1478-3726","authenticated-orcid":true,"given":"Masaaki","family":"Kuwajima","sequence":"first","affiliation":[{"name":"The University of Texas at Austin","place":["Austin, Texas, United States 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