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The corresponding changes in biomechanical properties and cellular functionalities of neuronal and glial cells due to bTBI remain largely unexplored. In this work, high blast overpressures (BOPs) of 14.5 psi (single shockwave) and 29.0 psi (double shockwave) were applied to adult hippocampal progenitor cells (AHPCs) in two different directions (overpressure applied from \u2018top-to-bottom\u2019 and \u2018bottom-to-top\u2019 direction on the cell culture petridish). The resultant alterations in structural, nanomechanical, and viscoelastic properties as well as cellular survival, proliferation, and differentiation were analyzed using atomic force microscopy (AFM) and immunocytochemistry (ICC). Double shockwave exposure from \u2018bottom-to-top\u2019 direction yielded reduced Young\u2019s modulus, surface roughness, and viscosity, causing significant actin cytoskeletal disruptions compared to \u2018top-to-bottom\u2019 direction. ICC results demonstrated that double shockwave exposure from \u2018top-to-bottom\u2019 direction caused populations of oligodendrocytes and immature neurons to decrease, while \u2018bottom-to-top\u2019 double shockwave exposure caused an increase in the percentage of immature neurons as shown by increased TuJ1-immunoreactivity which is interpreted as evidence that cells have committed to a neuronal lineage and entered an immature\/early neuronal stage. These findings emphasize the interplay among cellular differentiation, mechanics, and resilience of neuronal and glial cells to trauma in bTBI aftermath.<\/jats:p>","DOI":"10.1371\/journal.pone.0355739","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T17:41:41Z","timestamp":1786642901000},"page":"e0355739","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["The effects of blast-induced traumatic brain injury on brain cellular mechanics and 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Ghajar","year":"2000","journal-title":"Lancet"},{"issue":"2","key":"pone.0355739.ref010","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1017\/S1355617709991287","article-title":"Neuropsychological performance following a history of multiple self-reported concussions: a meta-analysis","volume":"16","author":"HG Belanger","year":"2010","journal-title":"J Int Neuropsychol Soc"},{"issue":"134","key":"pone.0355739.ref011","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.3003716","article-title":"Chronic traumatic encephalopathy in blast-exposed military veterans and a blast neurotrauma mouse model","volume":"4","author":"LE Goldstein","year":"2012","journal-title":"Sci Transl Med"},{"key":"pone.0355739.ref012","author":"T Hertenstein","year":"2025"},{"key":"pone.0355739.ref013","first-page":"1113","article-title":"Plasma membrane damage as a marker of neuronal injury","volume":"2009","author":"MC LaPlaca","year":"2009","journal-title":"Annu Int Conf IEEE Eng Med Biol Soc"},{"issue":"25","key":"pone.0355739.ref014","doi-asserted-by":"crossref","first-page":"5995","DOI":"10.1016\/j.biomaterials.2013.04.042","article-title":"3D matrix microenvironment for targeted differentiation of embryonic stem cells into neural and glial lineages","volume":"34","author":"CR Kothapalli","year":"2013","journal-title":"Biomaterials"},{"issue":"7","key":"pone.0355739.ref015","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1002\/biot.201400019","article-title":"Multipotent adult hippocampal progenitor cells maintained as neurospheres favor differentiation toward glial lineages","volume":"9","author":"J Oh","year":"2014","journal-title":"Biotechnol J"},{"issue":"5","key":"pone.0355739.ref016","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1016\/j.surg.2008.05.017","article-title":"Acute, regional inflammatory response after traumatic brain injury: Implications for cellular therapy","volume":"144","author":"MT 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Magdesian","year":"2012","journal-title":"Biophys J"},{"issue":"8","key":"pone.0355739.ref026","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1089\/089771501316919148","article-title":"Neurochemical characterization of traumatic brain injury in humans","volume":"18","author":"A Regner","year":"2001","journal-title":"J Neurotrauma"},{"issue":"22","key":"pone.0355739.ref027","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.1089\/neu.2010.1579","article-title":"Neurogenesis in adult human brain after traumatic brain injury","volume":"30","author":"W Zheng","year":"2013","journal-title":"J Neurotrauma"},{"key":"pone.0355739.ref028","doi-asserted-by":"crossref","first-page":"104513","DOI":"10.1016\/j.jmbbm.2021.104513","article-title":"Deformation of an airfoil-shaped brain surrogate under shock wave loading","volume":"120","author":"L Zhang","year":"2021","journal-title":"J Mech Behav Biomed Mater"},{"key":"pone.0355739.ref029","doi-asserted-by":"crossref","first-page":"26992","DOI":"10.1038\/srep26992","article-title":"Primary blast causes mild, moderate, severe and lethal TBI with increasing blast overpressures: Experimental rat injury model","volume":"6","author":"V Mishra","year":"2016","journal-title":"Sci Rep"},{"key":"pone.0355739.ref030","doi-asserted-by":"crossref","first-page":"5846","DOI":"10.1016\/j.jmrt.2023.02.143","article-title":"Design of shock wave attenuation effects on multi-impedance-matched laminated composites","volume":"23","author":"W Zhao","year":"2023","journal-title":"Journal of Materials Research and Technology"},{"key":"pone.0355739.ref031","volume-title":"Atomic force microscopy in cell biology","author":"L Wilson","year":"2002"},{"issue":"1","key":"pone.0355739.ref032","doi-asserted-by":"crossref","first-page":"19302","DOI":"10.1038\/s41598-025-04841-4","article-title":"Exploring the heterogeneity in glioblastoma cellular mechanics using in-vitro assays and atomic force microscopy","volume":"15","author":"N Masud","year":"2025","journal-title":"Sci Rep"},{"issue":"18","key":"pone.0355739.ref033","doi-asserted-by":"crossref","first-page":"4827","DOI":"10.1007\/s10853-005-2020-x","article-title":"A mechanical model for creep, recovery and stress relaxation in polymeric materials","volume":"40","author":"KS Fancey","year":"2005","journal-title":"J Mater Sci"},{"issue":"2","key":"pone.0355739.ref034","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.ymeth.2013.03.037","article-title":"Method for quantitative measurements of the elastic modulus of biological cells in AFM indentation experiments","volume":"60","author":"I Sokolov","year":"2013","journal-title":"Methods"},{"issue":"3","key":"pone.0355739.ref035","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.bpj.2014.06.033","article-title":"If cell mechanics can be described by elastic modulus: study of different models and probes used in indentation experiments","volume":"107","author":"N Guz","year":"2014","journal-title":"Biophys J"},{"key":"pone.0355739.ref036","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/s12668-016-0191-3","article-title":"Discrimination Between Normal and Cancerous Cells Using AFM","volume":"6","author":"M Lekka","year":"2016","journal-title":"Bionanoscience"},{"issue":"12","key":"pone.0355739.ref037","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1038\/nnano.2007.388","article-title":"Nanomechanical analysis of cells from cancer patients","volume":"2","author":"SE Cross","year":"2007","journal-title":"Nat Nanotechnol"},{"issue":"1","key":"pone.0355739.ref038","doi-asserted-by":"crossref","first-page":"5117","DOI":"10.1038\/s41598-017-05383-0","article-title":"Standardized Nanomechanical Atomic Force Microscopy Procedure (SNAP) for Measuring Soft and Biological Samples","volume":"7","author":"H Schillers","year":"2017","journal-title":"Sci Rep"},{"issue":"5","key":"pone.0355739.ref039","doi-asserted-by":"crossref","first-page":"140046","DOI":"10.1098\/rsob.140046","article-title":"Atomic force microscopy-based microrheology reveals significant differences in the viscoelastic response between malign and benign cell lines","volume":"4","author":"J Rother","year":"2014","journal-title":"Open Biol"},{"key":"pone.0355739.ref040","first-page":"1855","article-title":"Actin stress fibers--assembly, dynamics and biological roles","volume":"125","author":"S Tojkander","year":"2012","journal-title":"J Cell Sci"},{"issue":"11","key":"pone.0355739.ref041","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.1016\/j.cellsig.2013.07.006","article-title":"Filopodia as sensors","volume":"25","author":"CA Heckman","year":"2013","journal-title":"Cell Signal"},{"key":"pone.0355739.ref042","doi-asserted-by":"crossref","first-page":"3277","DOI":"10.1016\/B978-1-4160-5316-3.00328-2","article-title":"Biomechanical Basis of Traumatic Brain Injury","volume-title":"Youmans Neurological Surgery","author":"DF Meaney","year":"2011"},{"issue":"1779","key":"pone.0355739.ref043","doi-asserted-by":"crossref","first-page":"20180215","DOI":"10.1098\/rstb.2018.0215","article-title":"Tissue mechanics, an important regulator of development and disease","volume":"374","author":"NME Ayad","year":"2019","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"issue":"10","key":"pone.0355739.ref044","doi-asserted-by":"crossref","first-page":"108702","DOI":"10.1103\/PhysRevLett.103.108702","article-title":"Skull flexure from blast waves: a mechanism for brain injury with implications for helmet design","volume":"103","author":"WC Moss","year":"2009","journal-title":"Phys Rev Lett"},{"issue":"6","key":"pone.0355739.ref045","doi-asserted-by":"crossref","first-page":"061007","DOI":"10.1115\/1.3118765","article-title":"Simulation of blast-induced early-time intracranial wave physics leading to traumatic brain injury","volume":"131","author":"PA Taylor","year":"2009","journal-title":"J Biomech Eng"},{"issue":"3","key":"pone.0355739.ref046","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1007\/s10237-012-0421-8","article-title":"Mechanics of blast loading on the head models in the study of traumatic brain injury using experimental and computational approaches","volume":"12","author":"S Ganpule","year":"2013","journal-title":"Biomechanics and modeling in mechanobiology"},{"issue":"4","key":"pone.0355739.ref047","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1089\/neu.1995.12.669","article-title":"Mechanisms of basilar skull fracture","volume":"12","author":"JH McElhaney","year":"1995","journal-title":"J Neurotrauma"},{"key":"pone.0355739.ref048","doi-asserted-by":"crossref","unstructured":"Beitchman JA, Lifshitz J, Harris NG, Thomas TC, Lafrenaye AD, H\u00e5nell A. Spatial distribution of neuropathology and neuroinflammation elucidate the biomechanics of fluid percussion injury. bioRxiv. 2020. 2020\u201310.","DOI":"10.1101\/2020.10.05.325514"},{"issue":"10","key":"pone.0355739.ref049","article-title":"Evaluation of the Direction of External Force Input to the Skull and Its Influence on the Severity of Traumatic Brain Injury","volume":"17","author":"M Mori","year":"2025","journal-title":"Cureus"},{"issue":"4","key":"pone.0355739.ref050","first-page":"869","article-title":"Concepts of shock wave propagation","volume":"52","author":"GE Duvall","year":"1962","journal-title":"Bulletin of the Seismological Society of America"},{"issue":"1","key":"pone.0355739.ref051","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1097\/00001756-199412300-00039","article-title":"Increased transmitter amino acid concentration in human ventricular CSF after brain trauma","volume":"6","author":"AM Palmer","year":"1994","journal-title":"Neuroreport"},{"issue":"4906","key":"pone.0355739.ref052","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1126\/science.2567056","article-title":"The role of excitatory amino acids and NMDA receptors in traumatic brain injury","volume":"244","author":"AI Faden","year":"1989","journal-title":"Science"},{"key":"pone.0355739.ref053","doi-asserted-by":"crossref","first-page":"39610","DOI":"10.1038\/srep39610","article-title":"Mechanical stress activates NMDA receptors in the absence of agonists","volume":"7","author":"MM Maneshi","year":"2017","journal-title":"Sci Rep"},{"key":"pone.0355739.ref054","doi-asserted-by":"crossref","first-page":"533","DOI":"10.3389\/fncel.2019.00533","article-title":"Remembering Mechanosensitivity of NMDA Receptors","volume":"13","author":"LR Johnson","year":"2019","journal-title":"Front Cell Neurosci"},{"issue":"4","key":"pone.0355739.ref055","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1111\/j.1349-7006.2006.00172.x","article-title":"Shock wave induced cytoskeletal and morphological deformations in a human renal carcinoma cell line","volume":"97","author":"SF Moosavi-Nejad","year":"2006","journal-title":"Cancer Sci"},{"issue":"3","key":"pone.0355739.ref056","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1007\/s10237-015-0706-9","article-title":"Relationship between cell stiffness and stress fiber amount, assessed by simultaneous atomic force microscopy and live-cell fluorescence imaging","volume":"15","author":"N Gavara","year":"2016","journal-title":"Biomechanics and modeling in mechanobiology"},{"key":"pone.0355739.ref057","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.msec.2014.04.003","article-title":"Exploring the elasticity and adhesion behavior of cardiac fibroblasts by atomic force microscopy indentation","volume":"40","author":"B Codan","year":"2014","journal-title":"Mater Sci Eng C Mater Biol Appl"},{"issue":"12","key":"pone.0355739.ref058","doi-asserted-by":"crossref","first-page":"623","DOI":"10.5483\/BMBRep.2018.51.12.237","article-title":"Cellular machinery for sensing mechanical force","volume":"51","author":"C-G Lim","year":"2018","journal-title":"BMB Rep"},{"issue":"2","key":"pone.0355739.ref059","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.devcel.2010.07.018","article-title":"Stretchy proteins on stretchy substrates: the important elements of integrin-mediated rigidity sensing","volume":"19","author":"SW Moore","year":"2010","journal-title":"Dev Cell"},{"issue":"17","key":"pone.0355739.ref060","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1089\/neu.2012.2788","article-title":"Long-term in vivo imaging of viscoelastic properties of the mouse brain after controlled cortical impact","volume":"30","author":"T Boulet","year":"2013","journal-title":"J Neurotrauma"},{"issue":"11","key":"pone.0355739.ref061","doi-asserted-by":"crossref","first-page":"4263","DOI":"10.1523\/JNEUROSCI.19-11-04263.1999","article-title":"High tolerance and delayed elastic response of cultured axons to dynamic stretch injury","volume":"19","author":"DH Smith","year":"1999","journal-title":"J Neurosci"},{"issue":"6","key":"pone.0355739.ref062","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1016\/j.neuron.2015.02.041","article-title":"Traumatic brain injury and the neuronal microenvironment: a potential role for neuropathological mechanotransduction","volume":"85","author":"MA Hemphill","year":"2015","journal-title":"Neuron"},{"issue":"5","key":"pone.0355739.ref063","article-title":"Mild and repetitive very mild axonal stretch injury triggers cystoskeletal mislocalization and growth cone collapse","volume":"12","author":"YC Yap","year":"2017","journal-title":"PLoS One"},{"issue":"5","key":"pone.0355739.ref064","doi-asserted-by":"crossref","first-page":"859","DOI":"10.3171\/jns.2001.95.5.0859","article-title":"Mild head injury increasing the brain\u2019s vulnerability to a second concussive impact","volume":"95","author":"HL Laurer","year":"2001","journal-title":"J Neurosurg"},{"issue":"4","key":"pone.0355739.ref065","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1089\/08977150252932389","article-title":"Repeated mild brain injuries result in cognitive impairment in B6C3F1 mice","volume":"19","author":"SM DeFord","year":"2002","journal-title":"J Neurotrauma"},{"issue":"1","key":"pone.0355739.ref066","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1038\/s41392-023-01501-9","article-title":"Cellular mechanotransduction in health and diseases: from molecular mechanism to therapeutic targets","volume":"8","author":"X Di","year":"2023","journal-title":"Signal Transduct Target Ther"},{"key":"pone.0355739.ref067","doi-asserted-by":"crossref","first-page":"113378","DOI":"10.1016\/j.expneurol.2020.113378","article-title":"Blast exposure predisposes the brain to increased neurological deficits in a model of blast plus blunt traumatic brain injury","volume":"332","author":"A Aravind","year":"2020","journal-title":"Exp Neurol"},{"key":"pone.0355739.ref068","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1089\/neu.2022.0044","article-title":"Understanding Primary Blast Injury: High Frequency Pressure Acutely Disrupts Neuronal Network Dynamics in Cerebral Organoids","volume":"39","author":"MJ Silvosa","year":"2022","journal-title":"J Neurotrauma"},{"issue":"8","key":"pone.0355739.ref069","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1089\/neu.2015.4097","article-title":"Traumatic Brain Injury Severity Affects Neurogenesis in Adult Mouse Hippocampus","volume":"33","author":"X Wang","year":"2016","journal-title":"J 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Bielefeld","year":"2024","journal-title":"Nat Commun"},{"issue":"1","key":"pone.0355739.ref073","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s10439-011-0420-4","article-title":"Mild neurotrauma indicates a range-specific pressure response to low level shock wave exposure","volume":"40","author":"PJ Vandevord","year":"2012","journal-title":"Ann Biomed Eng"},{"issue":"01","key":"pone.0355739.ref074","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4236\/ojfd.2025.151001","article-title":"Study of Cylindrical Explosive Shock Waves at Air-Water Interface","volume":"15","author":"J Rajasekar","year":"2025","journal-title":"OJFD"},{"key":"pone.0355739.ref075","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s40349-016-0053-z","article-title":"In-vitro cell treatment with focused shockwaves-influence of the experimental setup on the sound field and biological reaction","volume":"4","author":"K Dietz-Laursonn","year":"2016","journal-title":"J Ther 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