{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T18:33:32Z","timestamp":1786732412663,"version":"build-2736575974"},"reference-count":29,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Bacterial vaginosis (BV) is a common vaginal infection associated with adverse pregnancy outcomes and increased susceptibility to sexually transmitted infections. This study evaluated the diagnostic performance of the BV Rapid Blue point-of-care test compared with Amsel\u2019s criteria and assessed maternal characteristics and pregnancy outcomes among BV-positive pregnant women at Dr. Slamet Regional Hospital, Garut.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>A cross-sectional diagnostic accuracy study was conducted among 131 pregnant women between April and May 2025. The diagnostic performance of BV Rapid Blue was evaluated using Amsel\u2019s criteria as the pragmatic clinical reference standard. Diagnostic accuracy parameters were calculated, and multivariate logistic regression was performed to identify factors associated with BV.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>BV prevalence was 61.1%. Most participants were aged 25\u201335, multigravida, and had high school education. All participants reported vaginal discharge, while only 3.1% reported a history of STIs. Common complications included preeclampsia (46.6%) and PROM (32.1%). BV Rapid Blue showed strong agreement with Amsel\u2019s criteria (p\u2009&lt;\u20090.0001), with sensitivity 95.4%, specificity 55.7%, PPV 51.3%, and NPV 96.1%. BV recurrence (OR = 4.29, p\u2009&lt;\u20090.0001) and secondary infection (OR = 2.56, p\u2009=\u20090.01) were significant predictors, while higher education was protective (OR = 0.52, p\u2009=\u20090.02).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>BV remains prevalent in pregnancy. BV Rapid Blue is a reliable, rapid diagnostic tool with high sensitivity and NPV. Early diagnosis may facilitate earlier clinical assessment and management, particularly in settings where laboratory-based diagnostics are limited.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0355948","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T17:55:26Z","timestamp":1786730126000},"page":"e0355948","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Association between bacterial vaginosis prevalence and maternal characteristics in pregnant women: Diagnostic comparison of BV Rapid Blue and Amsel\u2019s criteria"],"prefix":"10.1371","volume":"21","author":[{"given":"Harya","family":"Hermawan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhanny Primantara Johari","family":"Santoso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annisa Dewi","family":"Nugrahani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1785-6214","authenticated-orcid":true,"given":"Muhammad Alamsyah","family":"Aziz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"issue":"4","key":"pone.0355948.ref001","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.ajog.2018.11.1089","article-title":"Ecological dynamics of the vaginal microbiome in relation to health and disease","volume":"220","author":"S Greenbaum","year":"2019","journal-title":"Am J Obstet Gynecol"},{"issue":"1","key":"pone.0355948.ref002","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3920\/BM2018.0075","article-title":"Evidence-based mixture containing Lactobacillus strains and lactoferrin to prevent recurrent bacterial vaginosis: a double blind, placebo controlled, randomised clinical trial","volume":"10","author":"R Russo","year":"2019","journal-title":"Benef Microbes"},{"key":"pone.0355948.ref003","doi-asserted-by":"crossref","first-page":"91","DOI":"10.2147\/OAJC.S135439","article-title":"Contraceptive use and the risk of sexually transmitted infection: systematic review and current perspectives","volume":"9","author":"J Deese","year":"2018","journal-title":"Open Access J Contracept"},{"key":"pone.0355948.ref004","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.micpath.2018.11.046","article-title":"Bacterial vaginosis: An insight into the prevalence, alternative treatments regimen and it\u2019s associated resistance patterns","volume":"127","author":"A Javed","year":"2019","journal-title":"Microb Pathog"},{"issue":"5","key":"pone.0355948.ref005","first-page":"252","article-title":"Gardnerella vaginalis infection. 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Incorporating the prevalence of disease into the sample size calculation for sensitivity and specificity","volume":"3","author":"NM Buderer","year":"1996","journal-title":"Acad Emerg Med"},{"issue":"8","key":"pone.0355948.ref017","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1016\/j.jclinepi.2004.12.009","article-title":"Sample size calculation should be performed for design accuracy in diagnostic test studies","volume":"58","author":"A Flahault","year":"2005","journal-title":"J Clin Epidemiol"},{"issue":"11","key":"pone.0355948.ref018","doi-asserted-by":"crossref","DOI":"10.1136\/bmjopen-2016-012799","article-title":"STARD 2015 guidelines for reporting diagnostic accuracy studies: explanation and elaboration","volume":"6","author":"JF Cohen","year":"2016","journal-title":"BMJ Open"},{"key":"pone.0355948.ref019","unstructured":"Putri L, Triani V, Indra I. Prevalence of bacterial vaginosis in pregnant women at RSKIA Kota Bandung. In: World Congress of Dermatology 2019 Abstract Book, Milan, Italy, 2019. 4929."},{"issue":"3","key":"pone.0355948.ref020","first-page":"149","article-title":"Gambaran hasil pemeriksaan bacterial vaginosis pada ibu hamil di Puskesmas Jatinangor","volume":"5","author":"DP Sari","year":"2018","journal-title":"Althea Med J"},{"key":"pone.0355948.ref021","unstructured":"Handayani A, Santoso BI, Wahyuningsih NE. Prevalence of bacterial vaginosis in pregnant women in Makassar. In: 2020."},{"issue":"11","key":"pone.0355948.ref022","doi-asserted-by":"crossref","first-page":"2275","DOI":"10.3390\/jcm10112275","article-title":"Screening Pregnant Women for Bacterial Vaginosis Using a Point-of-Care Test: A Prospective Validation Study","volume":"10","author":"P Foessleitner","year":"2021","journal-title":"J Clin Med"},{"issue":"1","key":"pone.0355948.ref023","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1186\/s12905-025-03615-4","article-title":"BVBlue as a diagnostic instrument for the diagnosis of bacterial vaginosis: a systematic review","volume":"25","author":"II Wiguna","year":"2025","journal-title":"BMC Womens Health"},{"key":"pone.0355948.ref024","doi-asserted-by":"crossref","first-page":"1367233","DOI":"10.3389\/fcimb.2024.1367233","article-title":"The role of sialidases in the pathogenesis of bacterial vaginosis and their use as a promising pharmacological target in bacterial vaginosis","volume":"14","author":"L Chen","year":"2024","journal-title":"Front Cell Infect Microbiol"},{"issue":"4","key":"pone.0355948.ref025","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1093\/cid\/civ353","article-title":"Biofilms: An Underappreciated Mechanism of Treatment Failure and Recurrence in Vaginal Infections","volume":"61","author":"CA Muzny","year":"2015","journal-title":"Clin Infect Dis"},{"key":"pone.0355948.ref026","article-title":"Current Treatment of Bacterial Vaginosis-Limitations and Need for Innovation","author":"CS Bradshaw","year":"2016","journal-title":"J Infect Dis"},{"issue":"6","key":"pone.0355948.ref027","article-title":"Influence of persistent bacterial vaginosis on the vaginal microbiome and immune mediators","volume":"222","author":"D Nasioudis","year":"2020","journal-title":"Am J Obstet Gynecol"},{"issue":"5","key":"pone.0355948.ref028","article-title":"Sociodemographic and behavioral predictors of recurrent bacterial vaginosis among sexual health clinic patients in New York City, 2014\u20132018","volume":"18","year":"2023","journal-title":"PLoS One"},{"issue":"23","key":"pone.0355948.ref029","doi-asserted-by":"crossref","first-page":"4230","DOI":"10.3390\/nu16234230","article-title":"Probiotics in the Prophylaxis of Premature Rupture of Membranes and Cervical Incompetence","volume":"16","author":"C-C Vaduva","year":"2024","journal-title":"Nutrients"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0355948","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T17:55:32Z","timestamp":1786730132000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0355948"}},"subtitle":[],"editor":[{"given":"Benjamin Jerry","family":"Ridenhour","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,8,14]]},"references-count":29,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8,14]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0355948","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,14]]}}}