{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T20:46:03Z","timestamp":1786653963632,"version":"build-2736575974"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Amsterdam University Medical Center PhD grant"},{"name":"ZonMW-Vici grant 2019","award":["09150181910001"],"award-info":[{"award-number":["09150181910001"]}]},{"name":"Amsterdam University Medical Center Postdoc Career Bridging Grant"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Bacterial glycans are key components in immune interactions. We lack insight into the diverse glycan landscape present in complex microbial communities since current -omics techniques do not capture this post-translational information. Here we employed C-type lectin receptors (CLRs), which are dedicated innate glycan-sensing receptors, as probes for bacterial cell sorting in combination with 16S rRNA gene sequencing to identify microbiota species with a specific CLR-reactive glycan profile. We established our experimental CLR-sequencing (CLR-seq) pipeline using soluble fluorescently-labeled human macrophage galactose C-type lectin (MGL, CD301) and langerin (CD207). Both receptors identified known langerin- or MGL-interacting\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    strains in a synthetic microbial community even when present at low abundance. Subsequent application of CLR-seq on fecal microbiota samples from healthy donors identified specific langerin- and MGL-interacting bacterial species that were subsequently validated as monocultures. In summary, CLR-seq is a modular platform that allows identification of human microbiota species based on CLR-interacting glycans with easy expansion to other CLRs or microbiota samples from patients. Given that CLRs are densely expressed on dendrites of antigen-presenting cells, this pathway may play a role in cross-barrier recognition and sampling of the environment during homeostasis.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0356059","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T20:04:16Z","timestamp":1786651456000},"page":"e0356059","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["CLR-Seq: A pipeline to identify bacterial microbiota species with immune-relevant glycan moieties through human C-type lectin receptor interaction"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3536-3488","authenticated-orcid":true,"given":"Jasper","family":"Mol","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0436-6048","authenticated-orcid":true,"given":"Rob","family":"van Dalen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1154-652X","authenticated-orcid":true,"given":"Yvonne","family":"Pannekoek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5064-2350","authenticated-orcid":true,"given":"Malgorzata E.","family":"Mnich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6561-5810","authenticated-orcid":true,"given":"Marcel R.","family":"de 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