{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T18:07:59Z","timestamp":1789063679751,"version":"build-2803163510"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100022110","name":"bioM\u00e9rieux","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100022110","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Timely management of sepsis relies on rapid diagnosis of bloodstream infections (BSIs), including early pathogen identification and antimicrobial susceptibility testing (AST). The Vitek\n                    <jats:sup>\u00ae<\/jats:sup>\n                    REVEAL\n                    <jats:sup>TM<\/jats:sup>\n                    is an innovative rapid AST platform that detects volatile organic compounds emitted by Gram-negative bacilli (GNB) directly from positive blood cultures. This study assessed the analytical performance and clinical contribution of the BioFire\n                    <jats:sup>\u00ae<\/jats:sup>\n                    BCID2 molecular panel and the Vitek\n                    <jats:sup>\u00ae<\/jats:sup>\n                    REVEAL\n                    <jats:sup>TM<\/jats:sup>\n                    system in the diagnosis of GNB-BSI among intensive care unit (ICU) patients. We analyzed the prospective phase of the BacteREVEAL study, conducted between March and October 2023 at N\u00eemes University Hospital. Results from BioFire\n                    <jats:sup>\u00ae<\/jats:sup>\n                    BCID2 and Vitek\n                    <jats:sup>\u00ae<\/jats:sup>\n                    REVEAL\n                    <jats:sup>TM<\/jats:sup>\n                    were compared to standard-of-care methods, including MALDI-TOF identification and direct disk diffusion AST. Performance metrics for Vitek\n                    <jats:sup>\u00ae<\/jats:sup>\n                    REVEAL\n                    <jats:sup>TM<\/jats:sup>\n                    were evaluated according to ISO 20776\u20132:2007 standards. Among 94 ICU patients with a first episode of GNB-BSI, 50 monomicrobial cases were included.\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    was the most frequently identified species (n\u2009=\u200930, 60%), followed by\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    (n\u2009=\u20097, 14%). BioFire\n                    <jats:sup>\u00ae<\/jats:sup>\n                    BCID2 provided accurate identification for all isolates. The innovative tools reduced median turnaround times for identification by 19.0 h and for AST by 14.9 h. The mean time to result for Vitek\n                    <jats:sup>\u00ae<\/jats:sup>\n                    REVEAL\n                    <jats:sup>TM<\/jats:sup>\n                    was 5.7 h. Overall categorical agreement with standard AST was 97.7%, with 8 very major errors, 8 major errors, and 2 minor errors. These findings support the integration of BioFire\n                    <jats:sup>\u00ae<\/jats:sup>\n                    BCID2 and Vitek\n                    <jats:sup>\u00ae<\/jats:sup>\n                    REVEAL\n                    <jats:sup>TM<\/jats:sup>\n                    into routine workflows to accelerate GNB-BSI diagnosis and improve sepsis management.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0356199","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T17:29:54Z","timestamp":1789061394000},"page":"e0356199","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Real-life evaluation of BioFire\u00ae BCID2 and Vitek\u00ae REVEALTM for Gram-negative bloodstream infections in critical care"],"prefix":"10.1371","volume":"21","author":[{"given":"Massinissa","family":"Benyahia","sequence":"first","affiliation":[{"name":"N\u00eemes University Hospital","place":["N\u00eemes, France"],"department":["Department of Microbiology and Hospital Hygiene"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Roger","sequence":"additional","affiliation":[{"name":"UR-UM103 IMAGINE, University of Montpellier, Pain and Emergency Medicine, N\u00eemes University Hospital","place":["N\u00eemes, France"],"department":["Division of Anesthesia and Critical Care"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeline","family":"Boutet-Dubois","sequence":"additional","affiliation":[{"name":"N\u00eemes University Hospital","place":["N\u00eemes, France"],"department":["Department of Microbiology and Hospital Hygiene"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Salipante","sequence":"additional","affiliation":[{"name":"Epidemiology, Public Health, and Innovation in Methodology (BESPIM), N\u00eemes University Hospital","place":["N\u00eemes, France"],"department":["Department of Biostatistics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Oudiane","sequence":"additional","affiliation":[{"name":"UR-UM103 IMAGINE, University of Montpellier, Pain and Emergency Medicine, N\u00eemes University Hospital","place":["N\u00eemes, France"],"department":["Division of Anesthesia and Critical Care"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthieu","family":"Curtil Dit Galin","sequence":"additional","affiliation":[{"name":"N\u00eemes University Hospital","place":["N\u00eemes, France"],"department":["Department of Microbiology and Hospital Hygiene"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rim","family":"Nsiri","sequence":"additional","affiliation":[{"name":"N\u00eemes University Hospital","place":["N\u00eemes, France"],"department":["Department of Microbiology and Hospital Hygiene"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9o","family":"Brochon","sequence":"additional","affiliation":[{"name":"N\u00eemes University Hospital","place":["N\u00eemes, France"],"department":["Department of Microbiology and Hospital Hygiene"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Philippe","family":"Lavigne","sequence":"additional","affiliation":[{"name":"N\u00eemes University Hospital","place":["N\u00eemes, France"],"department":["Department of Microbiology and Hospital Hygiene"]},{"name":"VBIC, INSERM U1047, University of Montpellier","place":["N\u00eemes, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2317-4199","authenticated-orcid":true,"given":"Alix","family":"Pantel","sequence":"additional","affiliation":[{"name":"N\u00eemes University Hospital","place":["N\u00eemes, France"],"department":["Department of Microbiology and Hospital Hygiene"]},{"name":"VBIC, 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