{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:59:59Z","timestamp":1790704799276,"version":"4.1.0"},"reference-count":103,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The prevalence of vaping, defined as the use of e-cigarettes, during pregnancy is increasing, but its effects on maternal, fetal, and infant health remain incompletely understood. Human studies are heterogeneous and largely observational with a primary focus on neonatal and infant outcomes and limited assessment of maternal physiology. In contrast, pre-clinical animal studies suggest potential biological effects across maternal vascular, respiratory, metabolic, and neurodevelopmental systems. A comprehensive mapping of the existing evidence is therefore needed to characterise the scope of research to date and identify key gaps in the literature.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To map the extent, range, and nature of human and animal studies examining the effects of vaping during pregnancy on maternal, fetal, and infant health outcomes, and to describe key methodological features and gaps to inform future research.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>A scoping review was conducted following Joanna Briggs Institute methodology and PRISMA-ScR guidelines. Electronic database searches for human and animal studies examining the effects of vaping during pregnancy were conducted in MEDLINE, EMBASE, CINAHL, CENTRAL, PsycInfo, and grey literature, which were completed September 10th, 2025. Data were extracted on study design, population, exposure definitions, outcome domains, and use of biochemical exposure validation. No formal risk-of-bias assessment was undertaken, consistent with scoping review methodology, instead study characteristics were mapped descriptively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>64 studies were included (34 animal, 30 human). Human studies were predominantly observational and varied in design. Most relied on retrospective self-report without biochemical validation, potentially contributing to contradictory findings. Outcomes were focused mainly on perinatal measures, with maternal outcomes limited to breastfeeding, postpartum depression, and gestational weight gain, and little or no assessment of maternal physiological outcomes such as vascular or autonomic function. In contrast, animal studies frequently investigated mechanistic outcomes, suggesting potential biological effects across multiple systems including metabolic, respiratory, neurobehavioural, and cardiovascular impairment, with greater severity in nicotine-exposed groups. However, exposure paradigms and species differences limit direct translation to human pregnancy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec005\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The existing evidence base on vaping in pregnancy is characterised by substantial heterogeneity and notable gaps. In particular, there is a lack of prospective human studies incorporating objective exposure assessment and mechanistic maternal outcomes. Comparative analyses frequently evaluated vaping relative to combustible cigarette smoking. However, substantial variability in exposure classification, comparator groups, and adjustment for confounding limits interpretability. Animal studies more thoroughly examine mechanistic pathways and multiple organ systems. However physiological differences and unrealistic exposure models limit generalisability to human pregnancy. Overall, the current literature is insufficient to draw firm conclusions regarding risk. These findings highlight priorities for future research to strengthen the evidence base and improve understanding of the biological effects of vaping during pregnancy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0356305","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:24:04Z","timestamp":1790702644000},"page":"e0356305","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Vaping during pregnancy and maternal, fetal, and infant health outcomes: A scoping review of human and animal studies"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3154-6535","authenticated-orcid":true,"given":"Stella","family":"Fielder","sequence":"first","affiliation":[{"name":"Saint Mary\u2019s Hospital","place":["Manchester, United Kingdom"],"department":["Maternal and Fetal Health Research Centre 5th floor (Research)"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chloe","family":"Barnes","sequence":"additional","affiliation":[{"name":"Manchester University NHS Foundation Trust, Obstetrics","place":["Manchester, England, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Ormesher","sequence":"additional","affiliation":[{"name":"Saint Mary\u2019s Hospital","place":["Manchester, United Kingdom"],"department":["Maternal and Fetal Health Research Centre 5th floor (Research)"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Arrowsmith","sequence":"additional","affiliation":[{"name":"Manchester Metropolitan University","place":["Manchester, England, United Kingdom"],"department":["Department of Life Sciences, Faculty of Science and Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rehan","family":"Junejo","sequence":"additional","affiliation":[{"name":"Manchester Metropolitan University","place":["Manchester, England, United Kingdom"],"department":["Department of Life Sciences, Faculty of Science and Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Ussher","sequence":"additional","affiliation":[{"name":"University of Stirling","place":["Stirling, United Kingdom"],"department":["Institute of Social Marketing and Health"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4303-7845","authenticated-orcid":true,"given":"Alexander","family":"Heazell","sequence":"additional","affiliation":[{"name":"Saint Mary\u2019s Hospital","place":["Manchester, United Kingdom"],"department":["Maternal and Fetal Health Research Centre 5th floor (Research)"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"issue":"1","key":"pone.0356305.ref001","doi-asserted-by":"crossref","DOI":"10.1136\/fn.80.1.F8","article-title":"Effects of smoking in pregnancy on neonatal lung function","volume":"80","author":"AD Milner","year":"1999","journal-title":"Arch Dis Child Fetal Neonatal Ed"},{"issue":"3","key":"pone.0356305.ref002","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/S0749-3797(98)00089-0","article-title":"Effects of smoking during pregnancy. Five meta-analyses","volume":"16","author":"A Castles","year":"1999","journal-title":"Am J Prev Med"},{"issue":"5","key":"pone.0356305.ref003","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1093\/humupd\/dmr022","article-title":"Maternal smoking in pregnancy and birth defects: A systematic review based on 173 687 malformed cases and 11.7 million controls","volume":"17","author":"A Hackshaw","year":"2011","journal-title":"Hum Reprod Update"},{"issue":"2","key":"pone.0356305.ref004","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/sj.ijo.0803760","article-title":"Maternal smoking during pregnancy and child overweight: Systematic review and meta-analysis","volume":"32","author":"E Oken","year":"2008","journal-title":"Int J Obes (Lond)"},{"issue":"2","key":"pone.0356305.ref005","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.reprotox.2009.03.012","article-title":"Tobacco and pregnancy","volume":"28","author":"JM Rogers","year":"2009","journal-title":"Reprod Toxicol"},{"key":"pone.0356305.ref006","unstructured":"ASH. Fact sheet: Use of e-cigarettes (vapes) among adults in Great Britain. 2023. Available from: https:\/\/ash.org.uk\/uploads\/Use-of-e-cigarettes-among-adults-in-Great-Britain-2023.pdf"},{"key":"pone.0356305.ref007","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.neubiorev.2016.11.013","article-title":"Developmental toxicity of nicotine: A transdisciplinary synthesis and implications for emerging tobacco products","volume":"72","author":"LJ England","year":"2017","journal-title":"Neurosci Biobehav Rev"},{"issue":"2","key":"pone.0356305.ref008","doi-asserted-by":"crossref","DOI":"10.1152\/ajpheart.00738.2018","article-title":"Chronic intermittent electronic cigarette exposure induces cardiac dysfunction and atherosclerosis in apolipoprotein-E knockout mice","volume":"317","author":"J Espinoza-Derout","year":"2019","journal-title":"Am J Physiol Heart Circ Physiol"},{"issue":"5","key":"pone.0356305.ref009","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1172\/JCI5232","article-title":"Prenatal nicotine increases pulmonary alpha7 nicotinic receptor expression and alters fetal lung development in monkeys","volume":"103","author":"HS Sekhon","year":"1999","journal-title":"J Clin Invest"},{"issue":"2","key":"pone.0356305.ref010","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1111\/add.15185","article-title":"Fetal safety of nicotine replacement therapy in pregnancy: Systematic review and meta-analysis","volume":"116","author":"L Taylor","year":"2021","journal-title":"Addiction"},{"key":"pone.0356305.ref011","unstructured":"RCOG. Information for you smoking and pregnancy. 2015. Available from: https:\/\/www.rcog.org.uk\/media\/rvljrnjz\/pi-smoking-in-pregnancy.pdf"},{"key":"pone.0356305.ref012","unstructured":"ACOG. Tobacco and pregnancy. 2023. Available from: https:\/\/www.acog.org\/womens-health\/infographics\/tobacco-and-pregnancy"},{"issue":"4","key":"pone.0356305.ref013","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1097\/ADM.0000000000000128","article-title":"Knowledge, attitudes, and practice of electronic cigarette use among pregnant women","volume":"9","author":"KS Mark","year":"2015","journal-title":"J Addict Med"},{"issue":"6","key":"pone.0356305.ref014","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1111\/1471-0528.16553","article-title":"Pregnant women\u2019s use of e-cigarettes in the UK: A cross-sectional survey","volume":"128","author":"K Bowker","year":"2021","journal-title":"BJOG"},{"issue":"2","key":"pone.0356305.ref015","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1007\/s10900-021-01055-3","article-title":"Patterns and trends in use of electronic nicotine delivery systems before and during pregnancy: Pregnancy risk assessment monitoring system, United States, 2016-2019","volume":"47","author":"SK Head","year":"2022","journal-title":"J Community Health"},{"issue":"10","key":"pone.0356305.ref016","article-title":"Electronic cigarettes for smoking cessation","volume":"10","author":"J Hartmann-Boyce","year":"2020","journal-title":"Cochrane Database Syst Rev"},{"issue":"5","key":"pone.0356305.ref017","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1038\/s41591-022-01808-0","article-title":"Electronic cigarettes versus nicotine patches for smoking cessation in pregnancy: A randomized controlled trial","volume":"28","author":"P Hajek","year":"2022","journal-title":"Nat Med"},{"issue":"2","key":"pone.0356305.ref018","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1093\/ntr\/ntw160","article-title":"Exposure to nicotine and selected toxicants in cigarette smokers who switched to electronic cigarettes: A longitudinal within-subjects observational study","volume":"19","author":"ML Goniewicz","year":"2017","journal-title":"Nicotine Tob Res"},{"issue":"22","key":"pone.0356305.ref019","doi-asserted-by":"crossref","first-page":"4450","DOI":"10.3390\/ijerph16224450","article-title":"Analysis of toxic metals in liquid from electronic cigarettes","volume":"16","author":"N Gray","year":"2019","journal-title":"Int J Environ Res Public Health"},{"key":"pone.0356305.ref020","article-title":"Toxicant and nicotine exposure in pregnant smokers, vapers, and nicotine-replacement users: cross-sectional study","author":"M Ussher","year":"2025","journal-title":"Nicotine and Tobacco Research"},{"issue":"1","key":"pone.0356305.ref021","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1186\/s12884-024-06633-6","article-title":"Vaping during pregnancy: A systematic review of health outcomes","volume":"24","author":"M Ussher","year":"2024","journal-title":"BMC Pregnancy Childbirth"},{"key":"pone.0356305.ref022","doi-asserted-by":"crossref","first-page":"137028","DOI":"10.1016\/j.jhazmat.2024.137028","article-title":"Associations between vaping during pregnancy and perinatal outcomes: A systematic review and meta-analysis","volume":"486","author":"A Deprato","year":"2025","journal-title":"J Hazard Mater"},{"key":"pone.0356305.ref023","doi-asserted-by":"crossref","first-page":"138242","DOI":"10.1016\/j.jhazmat.2025.138242","article-title":"Letter to the Editor: Comments on \u201cAssociations between vaping during pregnancy and perinatal outcomes: A systematic review and meta-analysis\u201d by Deprato et al., (2025)","volume":"492","author":"M Ussher","year":"2025","journal-title":"J Hazard Mater"},{"key":"pone.0356305.ref024","doi-asserted-by":"crossref","first-page":"138591","DOI":"10.1016\/j.jhazmat.2025.138591","article-title":"Response to Comments on \u201cAssociations between vaping during pregnancy and perinatal outcomes: A systematic review and meta-analysis\u201d by Deprato et al. (2025)","volume":"494","author":"A Garud","year":"2025","journal-title":"J Hazard Mater"},{"issue":"1","key":"pone.0356305.ref025","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1093\/toxsci\/kfz194","article-title":"Neurological effects in the offspring after switching from tobacco cigarettes to e-cigarettes during pregnancy in a mouse model","volume":"172","author":"T Nguyen","year":"2019","journal-title":"Toxicol Sci"},{"issue":"16","key":"pone.0356305.ref026","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1002\/bdr2.1951","article-title":"Cardiovascular function during early development is suppressed by cinnamon flavored, nicotine-free, electronic cigarette vapor","volume":"113","author":"JM Piechowski","year":"2021","journal-title":"Birth Defects Res"},{"key":"pone.0356305.ref027","unstructured":"PRISMA. PRISma for scoping reviews (PRISMA-ScR): Preferred reporting items for systematic reviews and meta-analyses (PRISMA) 2018. 2018. Available from: https:\/\/www.prisma-statement.org\/scoping"},{"key":"pone.0356305.ref028","unstructured":"Peters MDJ, Godfrey C, McInerney P, Munn Z, Tricco AC, Khalil H. Scoping reviews (2020 version). In: Aromataris E, Munn Z, editors. JBI manual for evidence synthesis: JBI 2020. 2020."},{"key":"pone.0356305.ref029","doi-asserted-by":"crossref","DOI":"10.37766\/inplasy2024.9.0123","volume-title":"The effect of the use of e-cigarettes during pregnancy on maternal, fetal and infant health outcomes: A scoping review protocol","author":"S Fielder","year":"2024"},{"key":"pone.0356305.ref030","article-title":"5.2.4.3 Searching for grey literature","volume-title":"JBI Manual for Evidence Synthesis","author":"C Phillips","year":"2024"},{"issue":"1","key":"pone.0356305.ref031","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s12987-023-00416-5","article-title":"Maternal e-cigarette use can disrupt postnatal blood-brain barrier (BBB) integrity and deteriorates motor, learning and memory function: Influence of sex and age","volume":"20","author":"SR Archie","year":"2023","journal-title":"Fluids Barriers 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deficits","volume":"207","author":"MR Orzabal","year":"2019","journal-title":"Transl Res"},{"key":"pone.0356305.ref038","doi-asserted-by":"crossref","first-page":"100107","DOI":"10.1016\/j.metop.2021.100107","article-title":"Impact of gestational electronic cigarette vaping on amino acid signature profile in the pregnant mother and the fetus","volume":"11","author":"MR Orzabal","year":"2021","journal-title":"Metabol Open"},{"key":"pone.0356305.ref039","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.trsl.2022.03.009","article-title":"Impact of E-cig aerosol vaping on fetal and neonatal respiratory development and function","volume":"246","author":"MR Orzabal","year":"2022","journal-title":"Transl Res"},{"key":"pone.0356305.ref040","article-title":"Don\u2019t go vaping my heart: E-cigarette exposure during pregnancy promotes peripartum ventricular arrhythmias and sympathetic dominance","author":"BR Reynolds","year":"2025","journal-title":"American Journal of 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Lett"},{"key":"pone.0356305.ref056","doi-asserted-by":"crossref","first-page":"108673","DOI":"10.1016\/j.reprotox.2024.108673","article-title":"Perinatal nicotine vaping exposure induces pro-myofibroblastic phenotype in rat bone marrow-derived mesenchymal stem cells","volume":"129","author":"C Yu","year":"2024","journal-title":"Reprod Toxicol"},{"key":"pone.0356305.ref057","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1124\/jpet.122.289220","article-title":"Platelet function and gene expression in e-cigarette in utero exposed mice","volume":"385","author":"A Alarabi","year":"2023","journal-title":"The Journal of Pharmacology and Experimental Therapeutics"},{"issue":"7","key":"pone.0356305.ref058","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1021\/acs.chemrestox.8b00084","article-title":"Maternal e-cigarette exposure results in cognitive and epigenetic alterations in offspring in a mouse model","volume":"31","author":"T Nguyen","year":"2018","journal-title":"Chem Res Toxicol"},{"key":"pone.0356305.ref059","doi-asserted-by":"crossref","first-page":"112911","DOI":"10.1016\/j.physbeh.2020.112911","article-title":"Effect of electronic cigarette aerosol exposure during gestation and lactation on learning and memory of adult male offspring rats","volume":"221","author":"N Al-Sawalha","year":"2020","journal-title":"Physiol Behav"},{"issue":"3","key":"pone.0356305.ref060","doi-asserted-by":"crossref","first-page":"322","DOI":"10.3390\/genes15030322","article-title":"E-cigarette exposure alters neuroinflammation gene and protein expression in a murine model: Insights from perinatally exposed offspring and post-birth mothers","volume":"15","author":"C Awada","year":"2024","journal-title":"Genes (Basel)"},{"key":"pone.0356305.ref061","article-title":"Maternal e-cigarette vaping enhances development of allergic asthma in the offspring","volume-title":"Insights into Environmental Exposures in Asthma, Copd, and 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