{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T18:25:54Z","timestamp":1786991154365,"version":"build-2736575974"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Innovation Enhancement Project","award":["CXGC2026084"],"award-info":[{"award-number":["CXGC2026084"]}]},{"name":"the Shanxi Provincial Graduate Academic Innovation Project","award":["2025XS291"],"award-info":[{"award-number":["2025XS291"]}]},{"name":"Shanxi Province Key R&D Plan","award":["202302010101003"],"award-info":[{"award-number":["202302010101003"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    B-type Response Regulators (B-RRs) play a crucial regulatory role in cytokinin signal transduction and are significant for plant development and stress resistance. However, studies on\n                    <jats:italic>B-RRs<\/jats:italic>\n                    in tomato are limited. In this study, we conducted a comprehensive analysis of the\n                    <jats:italic>B-RRs<\/jats:italic>\n                    gene family in tomato. A total of 10 genes encoding B-RRs transcription factors (named as\n                    <jats:italic>SlRR1<\/jats:italic>\n                    to\n                    <jats:italic>SlRR10<\/jats:italic>\n                    ) were identified. We analyzed the structure, chromosomal localization, phylogeny, protein motifs, and expression levels of these\n                    <jats:italic>B-RRs<\/jats:italic>\n                    genes. Gene structure analysis revealed that the\n                    <jats:italic>B-RRs<\/jats:italic>\n                    genes in tomato possess between 4\u201311 exons and 4\u201312 introns. These genes are distributed across eight chromosomes. Phylogenetic tree analysis classified the\n                    <jats:italic>B-RRs<\/jats:italic>\n                    genes into three distinct subfamilies: subfamily I contains 6 genes that share five motifs, subfamily II includes 1 gene that shares three motifs, and subfamily III comprises 3 genes that share four motifs. Expression analysis demonstrated that\n                    <jats:italic>SlRR1<\/jats:italic>\n                    ,\n                    <jats:italic>SlRR2<\/jats:italic>\n                    , and\n                    <jats:italic>SlRR5<\/jats:italic>\n                    are highly expressed in the vegetative meristem, while\n                    <jats:italic>SlRR3<\/jats:italic>\n                    ,\n                    <jats:italic>SlRR4<\/jats:italic>\n                    , and\n                    <jats:italic>SlRR9<\/jats:italic>\n                    exhibit high expression levels in the roots.\n                    <jats:italic>SlRR7<\/jats:italic>\n                    was predominantly expressed in the fruits, whereas\n                    <jats:italic>SlRR6<\/jats:italic>\n                    ,\n                    <jats:italic>SlRR8<\/jats:italic>\n                    , and\n                    <jats:italic>SlRR10<\/jats:italic>\n                    show low expression or are undetectable in all tissues. Cis-element analysis revealed the presence of drought and abscisic acid (ABA) response elements in the promoters of the B-RRs genes. Correspondingly, their expression was significantly suppressed by both Polyethylene glycol (PEG) -induced drought stress and exogenous ABA treatment. Given the established antagonism between cytokinin and ABA, our findings suggest that tomato B-RRs may not only play a role in cytokinin signaling but also potentially serve as key nodes in the crosstalk between cytokinin and ABA pathways during drought stress responses. These findings indicate that the\n                    <jats:italic>B-RRs<\/jats:italic>\n                    genes in tomato are vital for plant growth, development, and stress resistance.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0356487","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:28:41Z","timestamp":1786987721000},"page":"e0356487","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-wide identification of tomato B-RRs gene family and expression analysis in response to ABA and drought"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7790-2227","authenticated-orcid":true,"given":"Meihua","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaofeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linlin","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxiu","family":"Miao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longqiang","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leiping","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"issue":"3","key":"pone.0356487.ref001","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.3390\/ijms26031037","article-title":"Multifunctional 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