{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T18:21:11Z","timestamp":1787854871016,"version":"build-2784847793"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Herein, a copolymeric hydrogel comprising a naturally occurring swellable biopolymer,\n                    <jats:italic>i.e.<\/jats:italic>\n                    , glucoxylan (a major polysaccharide of chia seed mucilage (CM)) and methacrylic acid (MA), was synthesized through free radical polymerization for wound healing applications. The formation of CM-co-MA was authenticated through FTIR spectroscopy. The CM-co-MA appeared non-hemolytic and non-thrombogenic, and exhibited noteworthy antioxidant potential with DPPH scavenging activity ranging from 18.68 to 47.70 \u00b5g AAE \u00b5g\/mL, FRAP activity from 32.22 to 63.70 \u00b5g AAE \u00b5g\/mL, TFC from 27.91 to 73.59 \u00b5g QE\/mL, and TPC from 26.95 to 58.21 \u00b5g GAE\/mL. The CM-co-MA showed antibacterial activities against\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    (Gram-positive) and\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (Gram-negative), having a zone of inhibition (ZOI) of 27.87 and 23.00 mm, respectively. The antifungal activities were recorded against\n                    <jats:italic>Aspergillus flavus<\/jats:italic>\n                    and\n                    <jats:italic>Candida albicans,<\/jats:italic>\n                    and found the length of growth inhibition of 72 and 76 mm, respectively. The acute oral toxicity study, acute dermal toxicity, and eye irritation test demonstrated the non-toxic, innocuous, and non-irritant attributes of the CM-co-MA, respectively. All animals stayed alive without any significant abnormalities during the 14 day study, and all hematological and biochemical parameters of the control and experimental group animals were comparable. Histopathology of the vital organs of rabbits also showed normal cellular architecture without any abnormalities. A wound healing study on rabbits revealed the tissue regeneration, with 84.40% recovery in treated rabbits after 14 days, and comparable with the positive control. Hence, the CM-co-MA is considered a non-toxic material having haemocompatibility, antioxidant, antibacterial, and antifungal activities for potential application in wound healing.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0356647","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T17:40:35Z","timestamp":1787852435000},"page":"e0356647","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synthesis and evaluation of haemocompatible, antioxidant, and antibacterial properties, and acute oral toxicity of a methacrylic acid mediated glucoxylan for wound healing applications"],"prefix":"10.1371","volume":"21","author":[{"given":"Samia","family":"Yaqoob","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arshad","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima Akbar","family":"Sheikh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1883-7226","authenticated-orcid":true,"given":"Muhammad Tahir","family":"Haseeb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hassan","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasir","family":"Assad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Farid-ul-Haq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Imran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Fakhar-e-Alam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2603-9989","authenticated-orcid":true,"given":"Ahmed","family":"Vandy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"pone.0356647.ref001","doi-asserted-by":"crossref","first-page":"127757","DOI":"10.1016\/j.ijbiomac.2023.127757","article-title":"Methyl cellulose\/okra mucilage composite films, functionalized with Hypericum perforatum oil and gentamicin, as a potential wound dressing","volume":"254","author":"SN Coban","year":"2024","journal-title":"Int J Biol Macromol"},{"key":"pone.0356647.ref002","first-page":"355","article-title":"Developing natural polymers for skin wound healing","volume":"33","author":"H Zhang","year":"2023","journal-title":"Bioact Mater"},{"issue":"1","key":"pone.0356647.ref003","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1080\/13880209.2023.2229379","article-title":"Metabolomics reveals the effects of hydroxysafflor yellow A on neurogenesis and axon regeneration after experimental traumatic brain injury","volume":"61","author":"E Hu","year":"2023","journal-title":"Pharm Biol"},{"issue":"16","key":"pone.0356647.ref004","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202100477","article-title":"Wound Healing: From Passive to Smart Dressings","volume":"10","author":"M Farahani","year":"2021","journal-title":"Adv Healthc Mater"},{"key":"pone.0356647.ref005","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.jconrel.2021.07.017","article-title":"Nanomaterials applied in wound healing: Mechanisms, limitations and perspectives","volume":"337","author":"M Wang","year":"2021","journal-title":"J Control Release"},{"issue":"32","key":"pone.0356647.ref006","doi-asserted-by":"crossref","DOI":"10.1002\/slct.202402073","article-title":"MXene\u2010Based Responsive Hydrogels and Applications in Wound Healing","volume":"9","author":"W Wang","year":"2024","journal-title":"ChemistrySelect"},{"key":"pone.0356647.ref007","doi-asserted-by":"crossref","first-page":"123036","DOI":"10.1016\/j.biomaterials.2024.123036","article-title":"Hydrogel-based nonwoven with persistent porosity for whole-stage hypertonic wound healing by regulating of water vaporization enthalpy","volume":"316","author":"Y Gao","year":"2025","journal-title":"Biomaterials"},{"issue":"15","key":"pone.0356647.ref008","doi-asserted-by":"crossref","first-page":"2402","DOI":"10.1080\/09205063.2024.2380570","article-title":"Current progress of protein-based dressing for wound healing applications - A review","volume":"35","author":"MUA Khan","year":"2024","journal-title":"J Biomater Sci Polym Ed"},{"key":"pone.0356647.ref009","article-title":"Progress and Application of Multifunctional Hydrogel in Radioactive Skin Injury","author":"X Cui","year":"2025","journal-title":"Adv. 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